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IRAK4 Antibody [Unconjugated]

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Western blot shows lysates of A431 human epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human IRAK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3919) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). For additional reference, recombinant human IRAK1, IRAK2, and IRAK4 (2 ng/lane) were included. A specific band for IRAK4 was detected at approxi­mately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of A431 human epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human IRAK4 Antigen Affinity-purified ...read more
IRAK4 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Goat Anti-Human IRAK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3919) at 15 µg/mL for 3 hours at room ...read more
Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and THP‑1 human acute monocytic leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for IRAK4 at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3919-SP
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IRAK4 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IRAK4
Met1-Ser460
Accession # Q9NWZ3
Specificity
Detects human IRAK4 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) IRAK1 and rhIRAK2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IRAK4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF3919 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IRAK4 Antibody [Unconjugated]

  • EC 2.7.11
  • EC 2.7.11.1
  • interleukin-1 receptor-associated kinase 4
  • IPD1
  • IRAK4
  • IRAK-4
  • NY-REN-64
  • REN64
  • Renal carcinoma antigen NY-REN-64

Background

IL-1 receptor-associated kinases (IRAKs) are serine/threonine kinases that help mediate signaling from Toll-like receptor (TLR) and IL-1 receptor family members. Four human IRAKs have been identified: IRAK1, IRAK2, IRAK-M, and IRAK4. Upon TLR ligand challenge, IRAK4 knockout mice exhibit severely impaired NF-kappa B activation. IRAK4 mutations have been described in patients with recurrent bacterial infections and poor inflammatory responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IRAK4 Antibody (AF3919)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.
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Bioinformatics

Gene Symbol IRAK4
Uniprot