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Integrin alpha 6/CD49f Antibody [Unconjugated]

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Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 1 ug/mL of Goat Anti-Human Integrin a6/CD49f Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1350) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Integrin a6/CD49f at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 1 ug/mL of Goat Anti-Human Integrin a6/CD49f Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1350) followed by HRP-conjugated ...read more
Simple Western lane view shows lysates of human kidney tissue (cortex), loaded at 0.2 mg/mL. A specific band was detected for Integrin alpha 6/CD49f at approximately 167 kDa (as indicated) using 10 µg/mL of Goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
Carrier Free
AF1350-SP
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Integrin alpha 6/CD49f Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Integrin alpha 6/CD49f
Pro93-Arg148, Ile557-Arg613
Accession # P23229
Specificity
Detects human Integrin alpha 6/CD49f in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Integrin alpha 9 is observed and less than 1% cross-reactivity with rhIntegrin  alpha 2, recombinant mouse (rm) Integrin  alpha 3, rhIntegrin  alpha 7, and rmIntegrin  alpha 7 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ITGA6
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF1350 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 6/CD49f Antibody [Unconjugated]

  • CD49 antigen-like family member F
  • CD49f antigen
  • CD49f
  • DKFZp686J01244
  • FLJ18737
  • Integrin alpha 6
  • integrin alpha-6
  • integrin alpha6B
  • integrin, alpha 6
  • ITGA6
  • ITGA6B
  • Platelet gpl
  • VLA-6

Background

Integrin alpha 6 (also VLA-6, CD49F and ITA6) is a 130‑150 kDa member of the integrin alpha chain family of molecules. Mature Integrin  alpha 6 is a 1107 amino acid (aa) type I transmembrane glycoprotein that contains a 1027 aa extracellular region and a 93 aa cytoplasmic tail The ECD is cleaved to generate a disulfide-linked dimer that shows a 125 kDa heavy chain (aa 24‑938), and a 30‑31 kDa membrane-bound light chain (aa 942‑1130). Integrin alpha 6 forms a nondisulfide-linked heterodimeric complex with both integrin beta 1 and beta 4. Each complex serves as a receptor for laminin. Splice variants exist that show either a deletion of aa 215‑258 or a deletion of aa 260‑298, possibly accompanied by a 29 aa substitution for aa 1084‑1130. Over aa 93‑148 and 518‑574, human Integrin alpha 6 shares 89% aa identity with mouse Integrin alpha 6.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Integrin alpha 6/CD49f Antibody (AF1350)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol ITGA6
Uniprot