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Integrin alpha 5/CD49e Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining in human placenta and skeletal muscle tissues using anti-ITGA5 antibody. Corresponding ITGA5 RNA-seq ...read more
Orthogonal Strategies: Western Blot: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Analysis in human cell lines U2OS and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading ...read more
Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining of human endometrium shows weak to moderate membranous positivity in smooth muscle cells.
Western Blot: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining of human placenta shows high expression.
Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining of human prostate shows moderate membranous positivity in smooth muscle cells.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Integrin alpha 5/CD49e Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DQPQKEEDLGPAVHHVYELINQGPSSISQGVLELSCPQALEGQQLLYVTRVTGLNCTTNHPINPKGLELDPEGSLHHQQKREAPSRSSASSGPQILKCPEAECFRLRCELGPLHQQESQSLQLHFRVWA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ITGA5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Integrin alpha 5/CD49e Protein (NBP1-84576PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-84576 in the following applications:

Publications
Read Publications using
NBP1-84576 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (82%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Integrin alpha 5/CD49e Antibody

  • CD49 antigen-like family member E
  • CD49e antigen
  • CD49e
  • Fibronectin receptor subunit alpha
  • fibronectin receptor, alpha subunit
  • FNRAVLA5A
  • Integrin alpha 5
  • integrin alpha-5
  • Integrin alpha-F
  • integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
  • ITGA5
  • very late activation protein 5, alpha subunit
  • VLA-5 alpha
  • VLA-5

Background

CD49e is a type I integral membrane glycoprotein, known as alpha5 integrin, VLA-5 alpha chain. It forms noncovalent heterodimer with integrin beta1 (CD29). CD49e contains two disulfide-linked subunits of 135 kDa and 24 kDa, and is mainly expressed on thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, and some myeloid leukemia cells. CD49e/CD29 complex binds to fibronection and neural adhesion molecule L1. It mediates monocytes migration, involves in the regulation of cells survival and apoptosis, and co-stimulates T cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Integrin alpha 5/CD49e Antibody (NBP1-84576)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IF/IHC, IHC-P.


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IF/IHC
(1)
IHC-P
(1)
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Mouse
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Review for Integrin alpha 5/CD49e Antibody (NBP1-84576) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-84576:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Integrin alpha 5/CD49e NBP1-84576
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4
reviewed by:
Verified Customer
WB Human 10/10/2012
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Summary

ApplicationWestern Blot
Sample TestedMDA-MB-231 Cell Lysate
SpeciesHuman

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FAQs for Integrin alpha 5/CD49e Antibody (NBP1-84576) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4
5
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4
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2
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1
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Verified Customer
10/10/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol ITGA5
Entrez
Uniprot