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Insulin Receptor beta Antibody

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Western Blot: Insulin Receptor beta Antibody [NB120-10991] - Representative lot data. Human brain lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Insulin Receptor, b subunit (1:1,000). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Insulin Receptor, b subunit (~97kDa)
Western Blot: Insulin Receptor beta Antibody [NB120-10991] - Representative lot data. Human brain lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Insulin Receptor, b subunit (1:1,000). ...read more
Immunoprecipitation: Insulin Receptor beta Antibody [NB120-10991] - Immunoprecipitation 10 ug of this antibody immunoprecipitated a-Insulin Receptor, Beta subunit from 500 micrograms of Hek293 cell lysate using protein ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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Insulin Receptor beta Antibody Summary

Immunogen
Protein corresponding to the last 100 C-terminal amino acid residues of the human insulin receptor beta chain. NCBI accession no. NP_000199.
Epitope
C-terminus
Localization
Type I membrane protein.
Specificity
Insulin Receptor beta
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
INSR
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunoprecipitation
  • Western Blot 1:100 - 1:500
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NIH 3T3 Whole Cell Lysate

Reactivity Notes

Chimpanzee.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1 M Tris-Glycine (pH 7.4), 0.15 M NaCl, 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Insulin Receptor beta Antibody

  • CD220 antigen
  • CD220
  • EC 2.7.10
  • EC 2.7.10.1
  • HHF5
  • insulin receptor
  • IR

Background

The Insulin Receptor (InsR) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. InsR is activated upon binding of the peptide hormone insulin, leading to autophosphorylation of tyrosine residues 1146, 1150, and 1151 in the activation loop of the beta-chain. Additional autophosphorylation sites such as tyrosine residues 960, 972, 1316, and 1322 regulate the assembly of signal transduction complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Control Lysate(s)

NIH 3T3 Whole Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol INSR
Entrez