![Immunohistochemistry-Paraffin: Insulin R/CD220 Antibody (INSR/2277R) [NBP3-07513] - Formalin-fixed, paraffin-embedded human Placenta stained with Insulin R/CD220 Rabbit Recombinant Monoclonal Antibody (INSR/2277R).](http://images.novusbio.com/fullsize/Insulin-R-CD220-Antibody-INSR-2277R-Immunohistochemistry-Paraffin-NBP3-07513-img0001.jpg "Immunohistochemistry-Paraffin: Insulin R/CD220 Antibody (INSR/2277R) [NBP3-07513] - Formalin-fixed, paraffin-embedded human Placenta stained with Insulin R/CD220 Rabbit Recombinant Monoclonal Antibody (INSR/2277R).")
| Reactivity | HuSpecies Glossary |
| Applications | IHC |
| Clone | INSR/2277R |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08838) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragment of extracellular domain of human Insulin Receptor alpha (exact sequence is proprietary) (Uniprot: P06213) |
| Localization | Cytoplasm |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | INSR |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 135 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
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