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IL-1 RI Antibody (129304) [Fluorescein]

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B220+mouse splenocytes were stained with Rat Anti-Mouse IL-1 RI Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB7712F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). View our protocol for Staining Membrane-associated Proteins.
B220+mouse splenocytes were stained with Rat Anti-Mouse IL-1 RI Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB7712F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). View our ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
129304
Clonality
Monoclonal
Host
Rat
Conjugate
Fluorescein

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB7712U-100UG
Alexa Fluor 405
FAB7712V-100UG
Alexa Fluor 488
FAB7712G-100UG
Alexa Fluor 532
FAB7712X
Alexa Fluor 594
FAB7712T-100UG
Alexa Fluor 647
FAB7712R-100UG
Alexa Fluor 700
FAB7712N-100UG
Alexa Fluor 750
FAB7712S-100UG
Allophycocyanin
FAB7712A
Allophycocyanin/Cy7
FAB7712APCCY7
Biotin
FAB7712B
DyLight 350
FAB7712D
DyLight 405
FAB7712E
DyLight 488
FAB7712K
DyLight 550
FAB7712L
DyLight 594
FAB7712M
DyLight 650
FAB7712W
DyLight 680
FAB7712Y
DyLight 755
FAB7712Z
Fluorescein
FAB7712F-025
HRP
FAB7712H
Janelia Fluor 525
FAB7712JF525
Janelia Fluor 549
FAB7712I
Janelia Fluor 646
FAB7712J
Janelia Fluor 669
FAB7712JF669
PE/Atto594
FAB7712PEATT594
PE/Cy5.5
FAB7712PECY55
PE/Cy7
FAB7712PECY7
PerCP
FAB7712C
Phycoerythrin
FAB7712P-025
Phycoerythrin
FAB7712P-100
Unconjugated
MAB7712
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB7712
Carrier Free
FAB7712P-100
Carrier Free
FAB7712F-025
Carrier Free
FAB7712P-025
Carrier Free
MAB7712
Carrier Free
MAB7712-SP
Carrier Free
FAB7712U-100UG
Carrier Free
FAB7712T-100UG
Carrier Free
FAB7712S-100UG
Carrier Free
FAB7712R-100UG
Carrier Free
FAB7712N-100UG
Carrier Free
FAB7712G-100UG
Carrier Free
FAB7712V-100UG
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IL-1 RI Antibody (129304) [Fluorescein] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-1 RI
Leu20-Lys338
Accession # P13504
Specificity
Detects mouse IL-1 RI in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
IL1R1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Publications
Read Publication using
FAB7712F in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 RI Antibody (129304) [Fluorescein]

  • CD121 antigen-like family member A
  • CD121a antigen
  • CD121a
  • IL-1 RI
  • IL1R
  • IL1R1
  • IL1RI
  • IL-1RI
  • IL1RT1
  • IL-1RT1
  • IL-1RT-1
  • Interleukin 1 Receptor 1
  • interleukin 1 receptor, type I
  • interleukin receptor 1
  • interleukin-1 receptor type 1
  • p80

Background

The type I IL-1 receptor (IL-1 RI, designated IL-1 R1 and CD121a) is one of at least nine members of the IL-1 R family within the Toll/IL-1 R (TIR) superfamily. IL‑1 RI is an ~80 kDa type I transmembrane (TM) protein that binds the pleiotropic cytokines IL-1 alpha and IL-1 beta , plus the IL-1 Receptor antagonist (IL-1 Ra). Signal transduction requires complex formation with the IL-1 R accessory protein (IL-1 R AcP/IL-1 R3), another type I TM protein. This complex recruits the adaptor protein MyD88 to initiate signaling in the NF kappa B pathway. Mouse IL-1 RI cDNA encodes a 576 amino acid (aa) protein that contains a 19 aa signal sequence, a 319 aa extracellular domain (ECD) with three C2-type Ig-like domains, a 21 aa TM domain and a 217 aa cytoplasmic region with a TIR domain. Mouse IL-1 RI shares 64%, 83%, 60%, 61% and 55% aa identity with human, rat, canine, equine and bovine IL-1 RI, respectively. The role of IL-1 in inflammation is under several levels of control, including expression and activation of IL-1 alpha and IL-1 beta , expression of IL-1 RI and its accessory and adaptor proteins, and negative regulators such as the IL‑receptor family member, IL-1 RII/IL-1 R2. IL-1 RI is expressed predominantly by T cells, fibroblasts, and endothelial cells and mediates acute phase inflammatory responses including fever.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-1 RI Antibody (FAB7712F)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow Cytometry.


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Bioinformatics

Gene Symbol IL1R1
Uniprot