Novus Biologicals products are now on bio-techne.com

ARHGEF5 Antibody

Images

 
Western Blot: ARHGEF5 Antibody [NB100-2559] - (0.1ug/ml) staining of HeLa Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ARHGEF5 Antibody [NB100-2559] - (3.75ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

ARHGEF5 Antibody Summary

Immunogen
Peptide with sequence C-DKEIDQNSQQEE corresponding to internal region according to NP_005426.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ARHGEF5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 170 kDa band observed in human HeLa lysates (calculated MW of 177 kDa band according to NP_005426.2).
IHC: Paraffin embedded Human Placenta.
Control
HeLa Whole Cell Lysate
Publications
Read Publication using NB100-2559.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ARHGEF5 Antibody

  • ephexin-3
  • GEF5
  • Guanine nucleotide regulatory protein TIM
  • Oncogene TIM
  • p60 TIM
  • P60
  • Rho guanine nucleotide exchange factor (GEF) 5
  • rho guanine nucleotide exchange factor 5
  • TIM1
  • TIMDKFZp686N1969
  • Transforming immortalized mammary oncogene

Background

Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-31470
Species: Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NB100-40853
Species: Hu
Applications: IHC, IHC-P, IP, WB
NBP2-24589
Species: Bv, Ca, Eq, Hu, Mu, Pm
Applications: IB, IHC, IHC-P, KO, WB
NBP1-82454
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
AF2365
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, Flow, IHC, KO, WB
AF2685
Species: Hu
Applications: IHC, Simple Western, WB
AF771
Species: Mu
Applications: IHC, Neut, WB
NBP1-81404
Species: Hu
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
NBP2-15939
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
AF1230
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
664-LI
Species: Hu
Applications: BA
NB200-106
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
AF2065
Species: Mu
Applications: ICC, IHC, Simple Western, WB
NBP1-87431
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-80965
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
H00010263-B01P
Species: Hu, Mu, Rt
Applications: WB
NBP1-32870
Species: Hu
Applications: IHC, IHC-P, IP, KD, WB
NBP1-71687
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P

Publications for ARHGEF5 Antibody (NB100-2559)(1)

Reviews for ARHGEF5 Antibody (NB100-2559) (0)

There are no reviews for ARHGEF5 Antibody (NB100-2559). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ARHGEF5 Antibody (NB100-2559) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional ARHGEF5 Products

Research Areas for ARHGEF5 Antibody (NB100-2559)

Find related products by research area.

Blogs on ARHGEF5

There are no specific blogs for ARHGEF5, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ARHGEF5 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol ARHGEF5
Entrez
Uniprot