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IFIT3 Antibody

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Western Blot: IFIT3 Antibody [NBP2-32500] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-IFIT3 antibody. Corresponding IFIT3 RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.
Orthogonal Strategies: Western Blot: IFIT3 Antibody [NBP2-32500] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-IFIT3 antibody. Corresponding IFIT3 RNA-seq data are presented for the same cell ...read more
Immunocytochemistry/ Immunofluorescence: IFIT3 Antibody [NBP2-32500] - Staining of human cell line A-431 shows localization to cytosol & mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: IFIT3 Antibody [NBP2-32500] - Staining of human skeletal muscle shows no positivity in myocytes.
Immunohistochemistry-Paraffin: IFIT3 Antibody [NBP2-32500] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: IFIT3 Antibody [NBP2-32500] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: IFIT3 Antibody [NBP2-32500] - Staining of human rectum shows moderate to strong cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

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IFIT3 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: HKQNGDLLQAAKCYEKELGRLLRDAPSGIGSIFLSASELEDGSEEMGQGAVSSSPRELLSNSEQL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IFIT3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
IFIT3 Protein (NBP2-32500PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for IFIT3 Antibody

  • CIG-49
  • GARG-49
  • IFI60Retinoic acid-induced gene G protein
  • IFIT-3
  • IFIT-4
  • IFIT4IFI-60K
  • interferon-induced protein with tetratricopeptide repeats 3
  • Interferon-induced protein with tetratricopeptide repeats 4Interferon-induced 60 kDa protein
  • IRG2
  • ISG60
  • ISG-60
  • RIG-GCIG49

Background

IFIT3, also known as IFIT4, IFI60, and RIGG, has been identified as a key element in the IFN-alpha anti-viral pathway. Additionally, IFIT3 binding negatively regulates the apoptotic effects of ISG54. IFIT3 is also a key target of STAT1, which is important in regulating IFN responses. One study has shown that expression of RIGG in a human monocytoma line led to growth arrest at the G1/S transition.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IFIT3