IL-1 beta/IL-1F2 Antibody - BSA Free

Interleukin-1 beta (IL-1 beta), also called IL-1F2, is a proinflammatory cytokine that functions in host defense following injury or inflammation (1-5). IL-1 beta is expressed by cells of the immune system such as monocytes, macrophage, neutrophils, and hepatocytes (1-3). Human IL-1 beta protein is expressed in the cytoplasm in an inactive precursor form (pro-IL-1 beta) which is 269 amino acids in length with a theoretical molecular weight of 31 kDa (1,4,6). Pro-IL-1 beta is cleaved by IL-1 converting enzyme (ICE), also called caspase-1, generating the 18 kDa mature, active protein that is secreted from cells (1,4,6). Pro-IL-1 beta is produced as a cellular response to molecular signals from pathogens and other processes termed pathogen associated molecular patterns (PAMPs) and damage associated molecular patterns (DAMPs) (1,2). PAMPs and DAMPs signal through pattern recognition receptors (PRRs) to induce formation of the inflammasome (1,2). The inflammasome is a multi-protein complex made of a PRR, such as Nod-like receptor family, pyrin domain containing 3(NLRP3), an adaptor, such as apoptosis-associated spec-like protein containing a CARD (ASC), and pro-caspase-1 (1-3,5). Assembly of the inflammasome enables cleavage of pro-caspase-1 into active caspase 1, followed by secretion of activate IL-1 beta (1-3,5).

IL-1 beta binding to its receptor IL-1RI and the downstream signaling contributes to a dual pathophysiological role (3). On one hand, IL-1 beta signaling activates immune cells and drives CD4+ T cell polarization to T helper type 1 (Th1) and Th17 cells, resulting in anti-tumor responses and mediation of acute inflammation (2,3). However, IL-1 beta also supports tumor growth and metastasis driven by multiple mechanisms including chronic inflammation, an immunosuppressive tumor microenvironment (TME), and angiogenesis (3). Additionally, IL-1 beta signaling been implicated in the pathogenesis of neuroinflammatory diseases of the central nervous system (CNS) such as multiple sclerosis (MS), Alzheimer's disease, and diabetic retinopathy (DR) (2). Mouse studies have shown regression of tumors treated with IL-1 as well as protective effects of IL-1 beta in instances of induced colitis and colon carcinoma (3). Conversely, blocking IL-1 beta has also shown promising effect in cancer treatment, especially when combined with chemotherapeutics (2,3). Approved IL-1 beta monoclonal antibody canakinumab has shown significant therapeutic promise in the treatment of DR (2). Given its multifaceted role in disease, IL-1 beta is a promising therapeutic target.

References

1. Lopez-Castejon G, Brough D. Understanding the mechanism of IL-1beta secretion. Cytokine Growth Factor Rev. 2011;22(4):189-195. https://doi.org/10.1016/j.cytogfr.2011.10.001

2. Mendiola AS, Cardona AE. The IL-1beta phenomena in neuroinflammatory diseases. J Neural Transm (Vienna). 2018;125(5):781-795. https://doi.org/10.1007/s00702-017-1732-9

3. Bent R, Moll L, Grabbe S, Bros M. Interleukin-1 Beta-A Friend or Foe in Malignancies?. Int J Mol Sci. 2018;19(8):2155. https://doi.org/doi:10.3390/ijms19082155

4. Krumm B, Xiang Y, Deng J. Structural biology of the IL-1 superfamily: key cytokines in the regulation of immune and inflammatory responses. Protein Sci. 2014;23(5):526-538. https://doi.org/10.1002/pro.2441

5. He Y, Hara H, Nunez G. Mechanism and Regulation of NLRP3 Inflammasome Activation. Trends Biochem Sci. 2016;41(12):1012-1021. https://doi.org/10.1016/j.tibs.2016.09.002

6. Uniprot (P01584)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ICC/IF, IF/IHC, IHC-P, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, WB.

Showing Publications 1 - 10 of 21. Show All 21 Publications. Collapse Publications.

Publications using NBP1-19775	Applications	Species
Jiang M, Shang Z, Zhang T et al. Study on the role of pyroptosis in bone resorption induced by occlusal trauma with or without periodontitis Journal of periodontal research 2022-02-09 [PMID: 35141913]
Tauber Z, Cizkova K. The anti-inflammatory role of placental Hofbauer cells is altered in patients with chorioamnionitis: Are CYP2C8 and soluble epoxide hydrolase involved in immunomodulation? Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 2022-09-14 [PMID: 33976432] (Immunohistochemistry)	Immunohistochemistry
Cizkova, K, Foltynkova, T Et al. Comparative Analysis of Immunohistochemical Staining Intensity Determined by Light Microscopy, ImageJ and QuPath in Placental Hofbauer Cells. Acta Histochem Cytochem 2021-02-25 [PMID: 33731967] (ICC/IF, WB, Human)	ICC/IF, WB	Human
Tauber Z, Chroma K, Baranova R, Cizkova K The expression patterns of IL-1beta and IL-10 and their relation to CYP epoxygenases in normal human placenta Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 2021-01-10 [PMID: 33440233] (IHC-P, Human)	IHC-P	Human
Ahmad A, Fauzia E, Kumar M et al. Gelatin-Coated Polycaprolactone Nanoparticle-Mediated Naringenin Delivery Rescue Human Mesenchymal Stem Cells from Oxygen Glucose Deprivation-Induced Inflammatory Stress ACS Biomater Sci Eng 2021-01-06 [PMID: 33405831]
Chen H, Wang B, Li G et al. Brain health is independently impaired by E-vaping and high-fat diet Brain Behav Immun 2020-11-20 [PMID: 33221488] (WB, Mouse) Details: Western blot analysis performed on brain lysates of mice who were exposed to e-vapor and a high-fat diet	WB	Mouse
AraUjo, L S, Torquato, B G S Et al. Renal expression of cytokines and chemokines in diabetic nephropathy. BMC Nephrol 2020-07-28 [PMID: 32723296] (ICC/IF, Human)	ICC/IF	Human
Cengiz O, Baran M, Balcioglu E et al. Use of selenium to ameliorate doxorubicin induced hepatotoxicity by targeting pro-inflammatory cytokines Biotech Histochem 2020-05-13 [PMID: 32400214]
Nam JE, Jo SY, Ahn CW, Kim YS Baicalin attenuates Fibrogenic process in human renal proximal tubular cells (HK-2) exposed to diabetic Milieu Life Sci. 2020-04-30 [PMID: 32360619] (WB, Human)	WB	Human
Gomez-Lopez N, Romero R, Tarca AL et al. Gasdermin D: Evidence of pyroptosis in spontaneous preterm labor with sterile intra-amniotic inflammation or intra-amniotic infection American Journal of Reproductive Immunology 2019-12-01 [PMID: 31461796] (Immunocytochemistry/ Immunofluorescence)	Immunocytochemistry/ Immunofluorescence
Ren G, Zheng X, Bommarito M et al. Gasdermin D: in vivo evidence of pyroptosis in spontaneous labor at term J. Matern. Fetal. Neonatal. Med. 2019-05-06 [PMID: 31006293] (IF/IHC, Human)	IF/IHC	Human
Gergin O, Bayram A, Gergin IS et al. Comparison of myotoxic effects of levobupivacaine, bupivacaine and ropivacaine: apoptotic activity and acute effect on pro-inflammatory cytokines Biotech Histochem. 2019-02-04 [PMID: 30712387] (IF/IHC, Rat)	IF/IHC	Rat
Gomez-Lopez N, Romero R, Panaitescu B et al. Inflammasome activation during spontaneous preterm labor with intra-amniotic infection or sterile intra-amniotic inflammation. Am. J. Reprod. Immunol. 2018-09-18 [PMID: 30225853] (ICC/IF, Human)	ICC/IF	Human
Coyac BR, Detzen L, Doucet P et al. Periodontal reconstruction by heparan sulfate mimetic-based matrix therapy in Porphyromonas gingivalis-infected mice Heliyon 2018-08-01 [PMID: 30101201] (IF/IHC, Mouse)	IF/IHC	Mouse
Gruber S, Arnold M, Cini N et al. Radioprotective Effects of Dermatan Sulfate in a Preclinical Model of Oral Mucositis-Targeting Inflammation, Hypoxia and Junction Proteins without Stimulating Proliferation. Int J Mol Sci. 2018-06-06 [PMID: 29882770] (IF/IHC, Mouse)	IF/IHC	Mouse
Kim JY, Lee H, Woo J et al. Reconstruction of pathway modification induced by nicotinamide using multi-omic network analyses in triple negative breast cancer. Sci Rep. 2017-06-14 [PMID: 28615672] (Human)		Human
Gruber S, Bozsaky E, Roitinger E et al. Early inflammatory changes in radiation-induced oral mucositis : Effect of pentoxifylline in a mouse model. Strahlenther Onkol. 2017-06-01 [PMID: 28258409] (IHC-P, Mouse)	IHC-P	Mouse
Lin C, Chao H, Li Z et al. Omega-3 fatty acids regulate NLRP3 inflammasome activation and prevent behavior deficits after traumatic brain injury Exp Neurol. 2017-04-01 [PMID: 28077339] (IF/IHC, Human)	IF/IHC	Human
Costa GM, de Oliveira AP, Martinelli PM et al. Demyelination/remyelination and expression of interleukin-1b, substance P, nerve growth factor, and glial-derived neurotrophic factor during trigeminal neuropathic pain in rats. Neurosci. Lett. 2015-12-11 [PMID: 26687274] (IHC-P, Rat)	IHC-P	Rat
Cicchini M, Chakrabarti R, Kongara S et al. Autophagy regulator BECN1 suppresses mammary tumorigenesis driven by WNT1 activation and following parity. Autophagy 2014-10-30 [PMID: 25483966] (IF/IHC)	IF/IHC
Omran A, Peng J, Zhang C et al. Interleukin-1 beta and microRNA-146a in an immature rat model and children with mesial temporal lobe epilepsy Epilepsia 2012-07-01 [PMID: 22708826] (WB, Rat, Human)	WB	Rat, Human
Show All 21 Publications. Collapse Publications.

Array NBP1-19775

• IL-1 beta/IL-1F2 Antibodies
• IL-1 beta/IL-1F2 ELISA Kit
• IL-1 beta/IL-1F2 Lysate
• IL-1 beta/IL-1F2 Proteins
• IL-1 beta/IL-1F2 ELISAs
• IL-1 beta/IL-1F2 Proteins and Enzymes