IKK alpha Antibody (14A231) - Azide Free

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkB is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, Ikka, Ikk2/, and Ikk3/. In vitro, Ikka and Ikk2/ can form homo- and heterodimers that can phosphorylate IkBs at the regulatory serine residues directly. Ikka and Ikk2/ are phosphorylated by NF-kB-inducing kinase (NIk) and MAP kinase kinase kinase-1 (MEkk1) (1), respectively. Targeted disruption of Ikka gene in mice results in skin and limb abnormalities and death of newborns (2,3).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.

Showing Publications 1 - 10 of 43. Show All 43 Publications. Collapse Publications.

Publications using NBP2-27409	Applications	Species
Fang X, Jeong JH, Long X et al. IKKalpha-mediated biogenesis of miR-196a through interaction with Drosha regulates the sensitivity of cancer cells to radiotherapy. Cell Death Differ. [PMID: 27058318] Details: Citation using the Azide Free form of this antibody.
Cui J, Li Y, Zhu L et al. NLRP4 negatively regulates type I interferon signaling by targeting the kinase TBK1 for degradation via the ubiquitin ligase DTX4. Nat Immunol. 2012-03-04 [PMID: 22388039]
Bakkar N, Ladner K, Canan BD et al. IKKa and alternative NF-kB regulate PGC-1B to promote oxidative muscle metabolism. J Cell Biol. 2012-02-20 [PMID: 22351927]
Delhase M, Kim SY, Lee H et al. TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2. Proc Natl Acad Sci U S A. 2012-01-24 [PMID: 22203995]
Phromnoi K, Reuter S, Sung B et al. A novel pentamethoxyflavone down-regulates tumor cell survival and proliferative and angiogenic gene products through inhibition of IkB kinase activation and sensitizes tumor cells to apoptosis by cytokines and chemotherapeutic agents. Mol Pharmacol. 2011-02-01 [PMID: 20930110] Details: KBM-5 cells: 1. FLIP (IMG-116-1, -2): WB (Fig 4A) 2. IKKb/IKK2 (IMG-129A): IP Kinase Assay (Fig 2D), IP/WB (Fig 2D). Note, the antibody IP'd an active kinase. The antibody co'IP'd IKKa. 3. IKKa (IMG-136a): WB (Figs 2C, 2D). Note Fig 2D was generated with an IP (IKKb, IMG-129A)/WB (IKKa, IMG-136a) assay. Note: The KBM-5 (human chronic myeloid leukemia) cells were incubated with PMF then treated with TNF-alpha, Figs 2C, 2D, 4A.
Harikumar KB, Sung B, Pandey MK et al. Escin, a pentacyclic triterpene, chemosensitizes human tumor cells through inhibition of nuclear factor-kappaB signaling pathway Mol Pharmacol 2010-05-01 [PMID: 20103608] Details: This citation used the Alexa Fluor 488 version of this antibody.
Huang J, Ren T, Guan H et al. HTLV-1 Tax is a critical lipid raft modulator that hijacks IkappaB kinases to the microdomains for persistent activation of NF-kappaB. J Biol Chem. 2009-03-06 [PMID: 19129196] Details: Antibodies cited: 1. IMX-5142 (IkBa- Sera): WB (human T cell lines), Fig. 1B. 2. IMG-136A (Ikka/Ikk1): WB (human T cell lines), Figs. 1E,D,G; 2A; 3D,E,F,G; 4B,F; 6C,D; 7C,D,G. 3. IMG-129A (Ikkb/Ikk2): WB (human T cell lines), Figs. 1E,D,G; 2A; 3D,E,F,G; 4
Kunnumakkara AB, Ichikawa H, Anand P et al. Coronarin D, a labdane diterpene, inhibits both constitutive and inducible nuclear factor-kappa B pathway activation, leading to potentiation of apoptosis, inhibition of invasion, and suppression of osteoclastogenesis. Mol Cancer Ther. 2008-10-01 [PMID: 18852134] (WB, Human) Details: IKK-alpha/IKK1 (IMG-136A): WB (human KMB-5 cells), Fig. 2C. 2. IKKb/IKK2 (IMG-129A): WB (human KMB-5 cells), Fig. 2C.	WB	Human
Eckmann L, Nebelsiek T, Fingerle AA et al. Opposing functions of IKKbeta during acute and chronic intestinal inflammation. Proc Natl Acad Sci U S A. 2008-09-30 [PMID: 18815378] (WB, Mouse) Details: WB (mouse colon cells), Fig. 4A.	WB	Mouse
Otipoby KL, Sasaki Y, Schmidt-Supprian M et al. BAFF activates Akt and Erk through BAFF-R in an IKK1-dependent manner in primary mouse B cells. Proc Natl Acad Sci U S A. 2008-08-26 [PMID: 18713867] (WB, Mouse) Details: WB (mouse splenic B cells), Fig. 1B.	WB	Mouse
Pandey MK, Sung B, Kunnumakkara AB et al. Berberine modifies cysteine 179 of IkappaBalpha kinase, suppresses nuclear factor-kappaB-regulated antiapoptotic gene products, and potentiates apoptosis. Cancer Res. 2008-07-01 [PMID: 18593939] Details: IMG-136A (IKKa/IKK1): WB (human multiple myeloma U266 cells), Fig. 3A, C. 2. IMG-129A (IKKb/IKK1): WB (human multiple myeloma U266 cells), Fig. 3A, C.
Kasinski AL, Du Y, Thomas SL et al. Inhibition of IkappaB kinase-nuclear factor-kappaB signaling pathway by 3,5-bis(2-flurobenzylidene)piperidin-4-one (EF24), a novel monoketone analog of curcumin. Mol Pharmacol. 2008-09-01 [PMID: 18577686] Details: Antibodies cited: 1. Ikka/Ikk1 (IMG-136A): IP (human A549 cells), immunoprecipitated cytoplasmic extracts were used for Ikk kinase assays. 2. Ikkb/Ikk2 (IMG-129A):WB (human A549 cells), Fig. 5a; 6a,b.
Beraza N, Ludde T, Assmus U et al. Hepatocyte-specific IKK gamma/NEMO expression determines the degree of liver injury. Gastroenterology. 2007-06-01 [PMID: 17570222] (WB, Mouse) Details: Products cited: 1. IMG-129A (Ikkb/Ikk2): IP (mouse liver proteins), Fig. 1E; Tab. 1. 2. IMG-136A (Ikka/Ikk1): WB (mouse live proteins), Fig. 1E.	WB	Mouse
Jackson-Bernitsas DG, Ichikawa H, Takada Y et al. Evidence that TNF-TNFR1-TRADD-TRAF2-RIP-TAK1-IKK pathway mediates constitutive NF-kappaB activation and proliferation in human head and neck squamous cell carcinoma. Oncogene. 2007-03-01 [PMID: 16953224]
Sethi G, Ahn KS, Sandur SK et al. Indirubin enhances tumor necrosis factor-induced apoptosis through modulation of nuclear factor-kappa B signaling pathway. J Biol Chem. 2006-08-18 [PMID: 16785236]
Ichikawa H, Aggarwal BB. Guggulsterone inhibits osteoclastogenesis induced by receptor activator of nuclear factor-kappaB ligand and by tumor cells by suppressing nuclear factor-kappaB activation. Clin Cancer Res. 2006-01-15 [PMID: 16428513]
Vinolo E, Sebban H, Chaffotte A et al. A point mutation in NEMO associated with anhidrotic ectodermal dysplasia with immunodeficiency pathology results in destabilization of the oligomer and reduces lipopolysaccharide- and tumor necrosis factor-mediated NF-kappa B activation. J Biol Chem. 2006-03-10 [PMID: 16379012]
Ichikawa H, Takada Y, Murakami A, Aggarwal BB. Identification of a novel blocker of I kappa B alpha kinase that enhances cellular apoptosis and inhibits cellular invasion through suppression of NF-kappa B-regulated gene products. J Immunol. 2005-06-01 [PMID: 15905586]
Ruocco MG, Maeda S, Park JM et al. I{kappa}B kinase (IKK){beta}, but not IKK{alpha}, is a critical mediator of osteoclast survival and is required for inflammation-induced bone loss. J Exp Med. 2005-05-16 [PMID: 15897281]
Nonaka M, Horie R, Itoh K et al. Aberrant NF-kappaB2/p52 expression in Hodgkin/Reed-Sternberg cells and CD30-transformed rat fibroblasts. Oncogene. 2005-06-02 [PMID: 15782119]
Panopoulos A, Harraz M, Engelhardt JF, Zandi E. Iron-mediated H2O2 production as a mechanism for cell type-specific inhibition of tumor necrosis factor alpha-induced but not interleukin-1beta-induced IkappaB kinase complex/nuclear factor-kappaB activation. J Biol Chem. 2005-01-28 [PMID: 15550384]
Shishodia S, Aggarwal BB. Guggulsterone inhibits NF-kappaB and IkappaBalpha kinase activation, suppresses expression of anti-apoptotic gene products, and enhances apoptosis. J Biol Chem. 2004-11-05 [PMID: 15322087]
Shishodia S, Koul D, Aggarwal BB. Cyclooxygenase (COX)-2 inhibitor celecoxib abrogates TNF-induced NF-kappa B activation through inhibition of activation of I kappa B alpha kinase and Akt in human non-small cell lung carcinoma: correlation with suppression of COX-2 synthesis. J Immunol. 2004-08-01 [PMID: 15265936]
Takada Y, Fang X, Jamaluddin MS et al. Genetic deletion of glycogen synthase kinase-3beta abrogates activation of IkappaBalpha kinase, JNK, Akt, and p44/p42 MAPK but potentiates apoptosis induced by tumor necrosis factor. J Biol Chem. 2004-09-17 [PMID: 15252041]
Chandrasekar B, Vemula K, Surabhi RM et al. Activation of intrinsic and extrinsic proapoptotic signaling pathways in interleukin-18-mediated human cardiac endothelial cell death. J Biol Chem. 2004-05-07 [PMID: 14960579] (WB, Human) Details: WB (human cardiac microvascular endothelial cells), Fig. 2G.	WB	Human
Takada Y, Singh S, Aggarwal BB. Identification of a p65 peptide that selectively inhibits NF-kappa B activation induced by various inflammatory stimuli and its role in down-regulation of NF-kappaB-mediated gene expression and up-regulation of apoptosis. J Biol Chem. 2004-04-09 [PMID: 14711835] (WB, Human)	WB	Human
Takada Y, Aggarwal BB. Flavopiridol inhibits NF-kappaB activation induced by various carcinogens and inflammatory agents through inhibition of IkappaBalpha kinase and p65 phosphorylation: abrogation of cyclin D1, cyclooxygenase-2, and matrix metalloprotease-9. J Biol Chem. 2004-02-06 [PMID: 14630924]
Saito N, Courtois G, Chiba A et al. Two carboxyl-terminal activation regions of Epstein-Barr virus latent membrane protein 1 activate NF-kappaB through distinct signaling pathways in fibroblast cell lines. J Biol Chem. 2003-11-21 [PMID: 12968033]
Chen LW, Egan L, Li ZW et al. The two faces of IKK and NF-kappaB inhibition: prevention of systemic inflammation but increased local injury following intestinal ischemia-reperfusion. Nat Med. 2003-05-01 [PMID: 12692538]
Majumdar S, Aggarwal BB. Adenosine suppresses activation of nuclear factor-kappaB selectively induced by tumor necrosis factor in different cell types. Oncogene. 2003-02-27 [PMID: 12606947]
Ashikawa K, Majumdar S, Banerjee S et al. Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation. J Immunol. 2002-12-01 [PMID: 12444159]
Chinanonwait N, Miura H, Yamamoto N, Yamaoka S. A recessive mutant cell line with a constitutive IkappaB kinase activity. FEBS Lett. 2002-11-20 [PMID: 12435610] (WB) Details: WB (Tk26 cells), Fig. 2c WB (Tk26 and M319-5 cells treated with cycloheximide), Fig. 3c WB (B5, Tk26, and hybrid Tk26--Rat-1 cells), Fig. 6b	WB
Dejardin E, Droin NM, Delhase M et al. The lymphotoxin-beta receptor induces different patterns of gene expression via two NF-kappaB pathways. Immunity. 2002-10-01 [PMID: 12387745]
Makris C, Roberts JL, Karin M. The carboxyl-terminal region of IkappaB kinase gamma (IKKgamma) is required for full IKK activation. Mol Cell Biol. 2002-09-01 [PMID: 12192055]
Anto RJ, Mukhopadhyay A, Shishodia S et al. Cigarette smoke condensate activates nuclear transcription factor-kappaB through phosphorylation and degradation of IkappaB(alpha): correlation with induction of cyclooxygenase-2. Carcinogenesis. 2002-09-01 [PMID: 12189195]
Majumdar S, Lamothe B, Aggarwal BB. Thalidomide suppresses NF-kappa B activation induced by TNF and H2O2, but not that activated by ceramide, lipopolysaccharides, or phorbol ester. J Immunol. 2002-03-15 [PMID: 11884428]
Agou F, Ye F, Goffinont S et al. NEMO trimerizes through its coiled-coil C-terminal domain. J Biol Chem. 2002-05-17 [PMID: 11877453]
Cao Y, Bonizzi G, Seagroves TN et al. IKKalpha provides an essential link between RANK signaling and cyclin D1 expression during mammary gland development. Cell. 2001-12-14 [PMID: 11747812]
Majumdar S, Aggarwal BB. Methotrexate suppresses NF-kappaB activation through inhibition of IkappaBalpha phosphorylation and degradation. J Immunol. 2001-09-01 [PMID: 11509639]
Miller BS, Zandi E. Complete reconstitution of human IkappaB kinase (IKK) complex in yeast. Assessment of its stoichiometry and the role of IKKgamma on the complex activity in the absence of stimulation. J Biol Chem. 2001-09-28 [PMID: 11470787]
Koul D, Yao Y, Abbruzzese JL et al. Tumor suppressor MMAC/PTEN inhibits cytokine-induced NFkappaB activation without interfering with the IkappaB degradation pathway. J Biol Chem. 2001-04-06 [PMID: 11278366]
Manna SK, Mukhopadhyay A, Aggarwal BB. Leflunomide suppresses TNF-induced cellular responses: effects on NF-kappa B, activator protein-1, c-Jun N-terminal protein kinase, and apoptosis. J Immunol. 2000-11-15 [PMID: 11067959]
Manna SK, Mukhopadhyay A, Aggarwal BB. IFN-alpha suppresses activation of nuclear transcription factors NF-kappa B and activator protein 1 and potentiates TNF-induced apoptosis. J Immunol. 2000-11-01 [PMID: 11046018]
Show All 43 Publications. Collapse Publications.

Array NBP2-27409

• IKK alpha Antibodies
• IKK alpha Lysate
• IKK alpha Proteins