IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - BSA Free

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. External stimuli such as tumor necrosis factor or other cytokines initiates a signal transduction cascade that leads to the activation of IkB-kinase complex that specifically phosphorylates IkBa on Serine-32 and Serine-36. Phosphorylation of these sites leads to ubiquitination of IkBa and subsequent degradation by the 26 S proteasome. Degradation of IkBa results in unmasking of the nuclear localization signal of NF-kB dimers, which subsequently translocates to the nucleus and activates target genes (7,8). Six members of IkB family members have been identified. One of the first gene induced following NF-kB activation is IkBa.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 2 applications: ICC/IF, WB.

Showing Publications 1 - 10 of 13. Show All 13 Publications. Collapse Publications.

Publications using NB100-56724	Applications	Species
Shahbazi S, Zakerali T The inhibitory role of benzo-dioxole-piperamide on the phosphorylation process as an NF-Kappa B silencer Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2021-11-28 [PMID: 34852990] (ICC/IF, WB, Human)	ICC/IF, WB	Human
Wen ZS, Tang Z, Gu LX et al. Immunomodulatory effect of low molecular-weight seleno-aminopolysaccharide on immunosuppressive mice. Int. J. Biol. Macromol. 2018-10-17 [PMID: 30342138] (WB, Mouse)	WB	Mouse
Kanno Y, Maruyama C, Matsuda A, Ishisaki A. uPA-derived peptide, A6 is involved in the suppression of lipopolysaccaride-promoted inflammatory osteoclastogenesis and the resultant bone loss. Immun Inflamm Dis 2017-05-11 [PMID: 28493442] (WB, Mouse)	WB	Mouse
Gu LX, Wen ZS, Xiang XW et al. Immunomodulatory effect of low molecular-weight seleno-aminopolysaccharides in intestinal epithelial cells. Int. J. Biol. Macromol. 2017-03-04 [PMID: 28267616] (WB, Porcine)	WB	Porcine
Samanta D, Gilkes D, Chaturvedi P, Xiang L, Semenza G. Hypoxia-inducible factors are required for chemotherapy resistance of breast cancer stem cells. Proc Natl Acad Sci U S A. 2014-12-01 [PMID: 25453096] (WB, Human)	WB	Human
Das U, Manna K, Sinha M et al. Role of ferulic Acid in the amelioration of ionizing radiation induced inflammation: a murine model. PLoS One 2014-05-24 [PMID: 24854039] (WB, Mouse)	WB	Mouse
Shakibaei Mehdi, Allaway David, Nebrich Simone, Mobasheri Ali. Botanical Extracts from Rosehip (Rosa canina), Willow Bark (Salix alba), and Nettle Leaf (Urtica dioica) Suppress IL-1beta-Induced NF-kappaB Activation in Canine Articular Chondrocytes. Evid Based Complement Alternat Med. 2012-01-01 [PMID: 22474508]
Li Q, Guo Y, Tan W et al. Cardioprotection afforded by inducible nitric oxide synthase gene therapy is mediated by cyclooxygenase-2 via a nuclear factor-kappaB dependent pathway. Circulation. 2007-10-02 [PMID: 17785622] (WB, Mouse) Details: WB (WT, COX-2-/-, IkBa (Ser 32A,Ser 36A) Tg mice injected with iNOS gene), Fig. 5.	WB	Mouse
Yang M, Hase H, Legarda-Addison D et al. B cell maturation antigen, the receptor for a proliferation-inducing ligand and B cell-activating factor of the TNF family, induces antigen presentation in B cells. J Immunol. 2005-09-01 [PMID: 16116167] (WB) Details: Antibodies cited [WB]: 1. IkBa Ser 32/Ser 36 [IMG-156A], (A20 B cells stimulated with APRIL), Fig. 2c 2. IkBa [IMG-127A], (A20 cells stimulated with anti-TNFR2), Fig. 6a	WB
Jazirehi AR, Huerta-Yepez S, Cheng G et al. Rituximab (chimeric anti-CD20 monoclonal antibody) inhibits the constitutive nuclear factor-{kappa}B signaling pathway in non-Hodgkin's lymphoma B-cell lines: role in sensitization to chemotherapeutic drug-induced apoptosis. Cancer Res. 2005-01-01 [PMID: 15665303] (WB) Details: WB (Ramos and Daudi cells growing in RPMI 1640 and 1% fetal bovine serum and rituximab), Fig. 4a	WB
Lotz Sonja, Aga Eresso, Wilde Inga et al. Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2. J Leukoc Biol. 2004-03-01 [PMID: 14673018]
Ikezoe T, Yang Y, Heber D et al. PC-SPES: a potent inhibitor of nuclear factor-kappa B rescues mice from lipopolysaccharide-induced septic shock. Mol Pharmacol. 2003-12-01 [PMID: 14645683]
Han HS, Karabiyikoglu M, Kelly S et al. Mild hypothermia inhibits nuclear factor-kappaB translocation in experimental stroke. J Cereb Blood Flow Metab. 2003-05-01 [PMID: 12771574] Details: Products cited for western blot in male Sprague-Dawley rats ischemic cortex, ischemic subcortex, contralateral cortex, and contralateral subcortex tissue. The rats were subject to mild and delayed hypothermia. 1. IKK-gamma (IMG-324A): Fig 4A & 4B. 2. IkB-
Show All 13 Publications. Collapse Publications.

Array NB100-56724

• IkB-alpha Antibodies
• IkB-alpha Antibody Pair
• IkB-alpha Lysate
• IkB-alpha Proteins
• IkB-alpha ELISA