![Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - Azide Free [NBP2-33247] - Analysis using the Biotin conjugate of NB100-56724. Detection of Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with this antibody. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/IkB-alpha-p-Ser32-p-Ser36-Antibody-39A1413-Azide-Free-Western-Blot-NBP2-33247-img0001.jpg "Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - Azide Free [NBP2-33247] - Analysis using the Biotin conjugate of NB100-56724. Detection of Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with this antibody. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, Rt, Po, Bv, CaSpecies Glossary |
| Applications | WB, IP |
| Clone | 39A1413 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide Free |
| Concentration | 1.0 mg/ml |
| Immunogen | This monoclonal antibody was raised against a synthetic peptide containing phosphorylated serines at amino acid residues 32 and 36 of human IkBa. |
| Modification | p Ser36, p Ser32 |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NFKBIA |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS - BSA free |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | NFKBIA |
![Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - Azide Free [NBP2-33247] - Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal) - a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lane 2) or without 1 nM TNF-a (lane 1). The membrane was blotted with NB100-56724 and immunoreactivity was detected by ECL. The data shows that NB100-56724 detects specifically the phosphorylated form of IkBa. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/IkB-alpha-p-Ser32-p-Ser36-Antibody-39A1413-Azide-Free-Western-Blot-NBP2-33247-img0002.jpg "Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - Azide Free [NBP2-33247] - Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal) - a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lane 2) or without 1 nM TNF-a (lane 1). The membrane was blotted with NB100-56724 and immunoreactivity was detected by ECL. The data shows that NB100-56724 detects specifically the phosphorylated form of IkBa. Image from the standard format of this antibody.")
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