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IFITM3/Fragilis Antibody

 
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Western Blot: IFITM3/Fragilis Antibody [NB600-1534] - Analysis of Fragilis in mouse ES lysate in the (1) absence and (2) presence of immunizing peptide and in mouse NIH3T3 lysate in the (3) absence and (4) presence of immunizing peptide using Fragilis antibody at 1 ug/ml.
Western Blot: IFITM3/Fragilis Antibody [NB600-1534] - Analysis of Fragilis in mouse ES lysate in the (1) absence and (2) presence of immunizing peptide and in mouse NIH3T3 lysate in the (3) absence and (4) presence of ...read more
Simple Western: IFITM3/Fragilis Antibody [NB600-1534] - Simple Western lane view shows a specific band for Fragilis in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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IFITM3/Fragilis Antibody Summary

Immunogen
A portion of amino acids 1-50 of mouse fragilis was used as the immunogen.
Localization
Cell Membrane
Marker
Primordial Germ Cell Marker
Specificity
Fragilis - Germ Line Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IFITM3
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Simple Western 1:10
  • Western Blot 0.5-2 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for IFITM3/Fragilis Antibody

  • 1-8U
  • Fragilis
  • IFITM3
  • interferon induced transmembrane protein 3 (1-8U)
  • interferon-induced transmembrane protein 3
  • Interferon-inducible protein 1-8U
  • IP15
  • mil-1

Background

Principal Names: Interferon-induced transmembrane protein 3(Ifitm3); Fragilis Official Gene Symbol: Ifitm3 Gen Bank Accession Number: NM_025378 Gene ID: 66141(Mouse) Gene Map Locus: 7F5 (Mouse) Fragilis is an IFN-induced transmembrane protein that belongs to the highly conserved fragilis protein family. It consists of an N-terminal signal sequence, 2 putative transmembrane domains, 3 potential phosphorylation sites and C-terminal Leucine-zipper. It is expressed in early embryos and nascent primordial germ cells and is associated with Germ cell specification and development. It is cell surface protein and might be important in mediating interactions amongst germ cells and their surrounding neighbours. It is also suggested that it might be involved in anti-proliferative signaling and homotypic cell adhesion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IFITM3
Uniprot