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Stella/Dppa3 Antibody [Unconjugated]

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Stella/Dppa3 was detected in perfusion fixed frozen sections of mouse ovary tissue using Goat Anti-Mouse Stella/Dppa3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2566) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to oocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Stella/Dppa3 was detected in perfusion fixed frozen sections of mouse ovary tissue using Goat Anti-Mouse Stella/Dppa3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2566) at 1 µg/mL overnight at 4 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF2566
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2566-SP
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Stella/Dppa3 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse Stella/Dppa3
Met1-Asn150
Accession # Q8QZY3
Specificity
Detects mouse Stella/Dppa3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant human Stella is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DPPA3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2566 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Stella/Dppa3 Antibody [Unconjugated]

  • developmental pluripotency associated 3
  • DPPA3 developmental pluripotency associated 3
  • Dppa3
  • Stella

Background

Stella, also known as Dppa3 (Developmental pluripotency-associated 3) and PGC7 (primordial germ cell 7) is expressed in primordial germ cells, oocytes, preimplantation embryos, and pluripotent cells. Although the fuction of Stella is not known, the expression pattern of the protein suggests a role in the maintenance of cell pluripotency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Stella/Dppa3 Antibody (AF2566)(5)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


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Bioinformatics

Gene Symbol DPPA3
Uniprot