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ICAM-1/CD54 Antibody [Biotin]

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 (3 Reviews)
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ICAM-1/CD54 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Mouse ICAM-1/CD54 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF796) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
ICAM-1/CD54 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Mouse ICAM-1/CD54 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF796) at 5 µg/mL ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF796
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF796
Carrier Free
AF796
Carrier Free
AF796-SP
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ICAM-1/CD54 Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ICAM‑1/CD54
Gln28-Asn485
Accession # Q3U8M7
Specificity
Detects mouse ICAM‑1/CD54 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross‑reactivity with recombinant human ICAM-1, recombinant rat ICAM-1, and recombinant mouse ICAM-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ICAM1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 2-10 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Mouse ICAM-1/CD54 Antibody (Catalog # MAB796)
ELISA Detection: Mouse ICAM-1/CD54 Biotinylated Antibody (Catalog # BAF796)
Standard: Recombinant Mouse ICAM-1/CD54 Fc Chimera (Catalog # 796-IC)
Readout System
Reviewed Applications
Read 3 Reviews rated 4.3
using
BAF796 in the following applications:
Publications
Read Publications using
BAF796 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICAM-1/CD54 Antibody [Biotin]

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma , TNF-alpha , IL-1 beta and LPS. Mouse and human ICAM-1 share approximately 54% amino acid identity.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer and transplantation.

  1. Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book. Academic Press, p. 74.
  2. Siu, G. et al. (1989) J. Immunol. 143:3813.
  3. Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICAM-1/CD54
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BAF796
ICAM-1/CD54 Antibody [Biotin]
Species: Mu
Applications: WB, IHC, ELISA(Det)

Publications for ICAM-1/CD54 Antibody (BAF796)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Luminex Development.


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Luminex Development
(1)
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Filter By Species
Mouse
(1)
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Reviews for ICAM-1/CD54 Antibody (BAF796) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using BAF796:
Filter by Applications
IF
(1)
IHC-P
(1)
IHC-Fr
(1)
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Mouse
(3)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 IF Mouse 01/15/2015
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Summary

ApplicationImmunofluorescence
Sample TestedSee PMID: 22198574
SpeciesMouse
  4
reviewed by:
Verified Customer
 IHC-P Mouse 01/15/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID: 22687510
SpeciesMouse
  4
reviewed by:
Verified Customer
 IHC-Fr Mouse 01/15/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID: 22198574
SpeciesMouse

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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
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 Verified Customer
01/15/2015
Application: IF
Species: Mouse
read this review
 
 Verified Customer
01/15/2015
Application: IHC-P
Species: Mouse
read this review
 
 Verified Customer
01/15/2015
Application: IHC-Fr
Species: Mouse
read this review

Bioinformatics

Gene Symbol ICAM1
Uniprot