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CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free

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Western Blot: CD58/LFA-3 Antibody (TS2/9) [NBP2-22542] - Analysis of 25 ug of Raji (Lane 1), Jurkat (Lane 2), and BAF-3 cell lysates (Lane 3) and a molecular weight protein ladder.
Immunocytochemistry/ Immunofluorescence: CD58/LFA-3 Antibody (TS2/9) [NBP2-22542] - Analysis of CD58 (green) showing staining in the cytoplasm of Raji cells (right) compared to a negative control without primary ...read more
Flow Cytometry: CD58/LFA-3 Antibody (TS2/9) [NBP2-22542] - Analysis of CD58 in BAF-3 cells compared to an isotype control (blue).
Flow Cytometry: CD58/LFA-3 Antibody (TS2/9) [NBP2-22542] - Analysis of CD58 in PBMC cells compared to an isotype control (blue).
Flow Cytometry: CD58/LFA-3 Antibody (TS2/9) [NBP2-22542] - Analysis of CD58 in PBMC cells compared to an isotype control (blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, B/N, CyTOF-ready
Clone
TS2/9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
0.98 mg/ml

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CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free Summary

Immunogen
Human Leukocyte Function Antigen-3
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD58
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Block/Neutralize
  • CyTOF-ready
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:100
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:500
Publications
Read Publication using NBP2-22542.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
0.98 mg/ml
Purity
Protein A purified

Alternate Names for CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free

  • Ag3
  • CD58 antigen
  • CD58 antigen, (lymphocyte function-associated antigen 3)
  • CD58 molecule
  • CD58
  • FLJ23181
  • FLJ43722
  • LFA3
  • LFA-3
  • LFA3ag3
  • lymphocyte function-associated antigen 3
  • Surface glycoprotein LFA-3

Background

LFA3 is a type 1 transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed on most hematopoietic cells and on nonhematopoietic cells such as fibroblasts, endothelial and epithelial cells. Its expression is particularly high on memory T-cells and dendritic cells. LFA3 expression on antigen-presenting cells and target cells enhances T-cells antigen recognition through binding to its ligand, LFA2/CD2, on T-cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD58/LFA-3 Antibody (NBP2-22542)(1)

We have publications tested in 1 application: Western Blot.


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Western Blot
(1)
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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD58
Entrez
Uniprot