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Human/Mouse/Rat Total AMPK alpha 1 DuoSet IC ELISA, 2 Plate

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Lysates prepared from MCF-7 human breast cancer cells, MDA-MB-468 human breast cancer cells, and HeLa human cervical epithelial carcinoma cells were quantified with this DuoSet IC ELISA. The same lysates were also ...read more
Lysates prepared from C2C12 mouse myoblast cells, DA3 mouse myeloma cells, and PC-12 rat adrenal pheochromocytoma cells were quantified with this DuoSet IC ELISA. The same lysates were also immunoblotted (inset) with ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ELISA
Conjugate
Biotin

Order Details

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Catalog# & Conjugate Size Price
Biotin
DYC3197-5
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
DYC3197-5
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Human/Mouse/Rat Total AMPK alpha 1 DuoSet IC ELISA, 2 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
PRKAA1

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DYC3197-2.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human/Mouse/Rat Total AMPK alpha 1 DuoSet IC ELISA, 2 Plate

  • AMP -activate kinase alpha 1 subunit
  • AMP-activated protein kinase, catalytic, alpha-1,5'-AMP-activated protein kinase catalytic subunit alpha-1
  • AMPK alpha 1
  • AMPK alpha 1,5'-AMP-activated protein kinase, catalytic alpha-1 chain
  • AMPK subunit alpha-1
  • AMPK
  • AMPK1
  • AMPKa1
  • EC 2.7.11
  • EC 2.7.11.1
  • MGC33776
  • MGC57364
  • PRKAA1
  • protein kinase, AMP-activated, alpha 1 catalytic subunit

Background

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic a subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms (alpha 1, alpha 2, beta 1, beta 2, gamma 1, gamma 2, gamma 3), with all 12 combinations capable of forming complexes. The catalytic a subunit of AMPK is activated allosterically by AMP and by phosphorylation via the AMPK kinase LKB1. Active AMPK downregulates anabolic pathways such as fatty acid, triglyceride, and cholesterol synthesis, and up-regulates catabolic pathways such as glycolysis and fatty acid oxidation. AMPK's role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.

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Human/Mouse/Rat Total AMPK alpha 1 DuoSet IC ELISA, 2 Plate
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Publications for AMPK alpha 1 (DYC3197-2)(2)

We have publications tested in 2 confirmed species: Human, Rat.


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Bioinformatics

Gene Symbol PRKAA1