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HSP70/HSPA1A Antibody [Unconjugated]

 (14 Publications)
 
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Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, L-929 mouse fibroblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat HSP70/HSPA1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1663) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HSP70/HSPA1A at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, L-929 mouse fibroblast cell line, and C6 rat glioma cell line. PVDF membrane was probed ...read more
Simple Western shows lysates of Exosome Standards (HT‑29) (NBP3-11685), Exosome Standards (Human Urine) (NBP2-49840) and human liver tissue, loaded at 0.5 mg/ml. A specific band was detected for HSP70/HSPA1A at ...read more
Biological Strategies: Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with heat shocked (HS), loaded at 0.2 mg/mL. A specific band was detected ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 66 kDa (as ...read more
Biological Strategies: RFA increases HSP70 levels and activates MyD88. a C57BL/6 mice were exposed to RF and skin HSP70, HSc70, and HSP90 levels were then analyzed by western blotting at 6 and 24 h (hr) using ...read more
Biological Strategies: RFA increases HSP70 levels and activates MyD88. a C57BL/6 mice were exposed to RF and skin HSP70, HSc70, and HSP90 levels were then analyzed by western blotting at 6 and 24 h (hr) using ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
   

Biological Strategies

      

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF1663-SP
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HSP70/HSPA1A Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human HSP70
Met1-Asp641
Accession # P08107
Specificity
Detects the induced form of recombinant endogenous human, mouse and rat HSP70/HSPA1A (HSP72) in Western blots. In Western blots, no cross‑reactivity with the constitutively expressed HSC70 (HSP73) is detected.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPA1A
Purity Statement
Antigen and protein A Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Simple Western 0.5 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF1663 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HSP70/HSPA1A Antibody [Unconjugated]

  • dnaK-type molecular chaperone HSP70-1
  • FLJ54303
  • FLJ54370
  • FLJ54392
  • FLJ54408
  • FLJ75127
  • Heat shock 70 kDa protein 1/2
  • heat shock 70 kDa protein 1A/1B
  • heat shock 70kD protein 1A
  • heat shock 70kDa protein 1A
  • heat shock-induced protein
  • HSP70
  • HSP70.1/HSP70.2
  • HSP70-1
  • HSP70-1/HSP70-2
  • HSP70-1A
  • HSP70I
  • HSP72
  • HSPA1
  • HSPA1A
  • HSPA1B

Background

Heat shock proteins (HSPs) are a family of highly conserved stress response proteins. Heat shock proteins function primarily as molecular chaperones by facilitating the folding of other cellular proteins, preventing protein aggregation or targeting improperly folded proteins to specific degradative pathways. HSPs are typically expressed at low levels under normal physiological conditions but are dramatically up‑regulated in response to cellular stress. HSP70 is a 72 kDa member of the heat shock protein 70 family of proteins.  HSP70, also known as HSPA1A, HSP70-1, and HSP72 is a 641 amino acid (aa) heat shock protein. Over aa 1-641, human HSP70 shares 95% and 97% aa identity to mouse and rat HSP70.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF1663
HSP70/HSPA1A Antibody [Unconjugated]
Species: Hu, Mu, Rt
Applications: WB, Simple Western

Publications for HSP70/HSPA1A Antibody (AF1663)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: IHC, IHC-P, Simple Western, Western Blot.


Filter By Application
IHC
(1)
IHC-P
(1)
Simple Western
(1)
Western Blot
(9)
All Applications
Filter By Species
Human
(5)
Mouse
(4)
Rat
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using AF1663 Applications Species
Pizzolato, G;Moparthi, L;Pagella, P;Cantù, C;D'Arcy, P;Koch, S; The tumour suppressor p53 is a negative regulator of the carcinoma-associated transcription factor FOXQ1 The Journal of biological chemistry 2024-03-01 [PMID: 38432629] (Western Blot, Human) Western Blot Human
Li J, Jaiswal MK, Chien JF et al. Divergent single cell transcriptome and epigenome alterations in ALS and FTD patients with C9orf72 mutation Nat Commun 2023-09-15 [PMID: 37714849] (Simple Western, Human) Simple Western Human
Lavanya Moparthi, Stefan Koch FOX transcription factors are common regulators of Wnt/ beta -catenin–dependent gene transcription Journal of Biological Chemistry 5/1/2023 [PMID: 37011861]
G Pizzolato, L Moparthi, S Söderholm, C Cantù, S Koch The oncogenic transcription factor FOXQ1 is a differential regulator of Wnt target genes Journal of Cell Science, 2022-10-10;0(0):. 2022-10-10 [PMID: 36124643] (Western Blot, Human) Western Blot Human
LE Martínez, S Lensing, D Chang, LI Magpantay, R Mitsuyasu, RF Ambinder, JA Sparano, O Martínez-M, M Epeldegui Plasma extracellular vesicles bearing PD-L1, CD40, CD40L or TNF-RII are significantly reduced after treatment of AIDS-NHL Oncogene, 2022-06-02;12(1):9185. 2022-06-02 [PMID: 35655072] (Western Blot, Human) Western Blot Human
E Clabough, J Ingersoll, T Reekes, A Gleichsner, A Ryan Acute Ethanol Exposure during Synaptogenesis Rapidly Alters Medium Spiny Neuron Morphology and Synaptic Protein Expression in the Dorsal Striatum International Journal of Molecular Sciences, 2021-12-28;23(1):. 2021-12-28 [PMID: 35008713] (Western Blot, Mouse) Western Blot Mouse
Y Lyu, S Kopcho, M Mohan, CM Okeoma Long-Term Low-Dose Delta-9-Tetrahydrocannbinol (THC) Administration to Simian Immunodeficiency Virus (SIV) Infected Rhesus Macaques Stimulates the Release of Bioactive Blood Extracellular Vesicles (EVs) that Induce Divergent Structural Adaptations and Signaling Cues Cells, 2020-10-06;9(10):. 2020-10-06 [PMID: 33036231] (Western Blot, Human) Western Blot Human
Solga R, Behrens J, Ziemann A et al. CRN2 binds to TIMP4 and MMP14 and promotes perivascular invasion of glioblastoma cells European Journal of Cell Biology 10/1/2019 [PMID: 31677819]
H Gerber, S Mosser, B Boury-Jamo, M Stumpe, A Piersigill, C Goepfert, J Dengjel, U Albrecht, F Magara, PC Fraering The APMAP interactome reveals new modulators of APP processing and beta-amyloid production that are altered in Alzheimer's disease Acta Neuropathol Commun, 2019-01-31;7(1):13. 2019-01-31 [PMID: 30704515] (Western Blot, Mouse) Western Blot Mouse
YO Son, HE Kim, WS Choi, CH Chun, JS Chun RNA-binding protein ZFP36L1 regulates osteoarthritis by modulating members of the heat shock protein 70 family Nat Commun, 2019-01-08;10(1):77. 1/8/2019 [PMID: 30622281]
Show All 14 Publications.

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Bioinformatics

Gene Symbol HSPA1A
Uniprot