HSP70/HSPA1A Antibody (C92F3A-5)

Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsp70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Porcine.

We have publications tested in 1 application: WB.

Showing Publications 1 - 8 of 8.

Publications using NB110-61582	Applications	Species
Ainhoa Martínez-Pizarro, Sara Picó, Arístides López-Márquez, Claudia Rodriguez-López, Elena Montalvo, Mar Alvarez, Margarita Castro, Santiago Ramón-Maiques, Belén Pérez, José J Lucas, Eva Richard, Lourdes R Desviat PAH deficient pathology in humanized c.1066-11G>A phenylketonuria mice Human Molecular Genetics 2024-06-15 [PMID: 38520741]
Sheng-Mou Hou, Chih-Yang Lin, Yi-Chin Fong, Chih-Hsin Tang Hypoxia-regulated exosomes mediate M2 macrophage polarization and promote metastasis in chondrosarcoma Aging (Albany NY) 2023-11-30 [PMID: 37993261]
Midekessa G, Godakumara K, Ord J et al. Zeta Potential of Extracellular Vesicles: Toward Understanding the Attributes that Determine Colloidal Stability ACS Omega 2020-07-14 [PMID: 32685837] (WB, Human)	WB	Human
Vilas A, Yuste-Checa P, Gallego D et Al. Proteostasis regulators as potential rescuers of PMM2 activity Biochim Biophys Acta Mol Basis Dis 2020-03-25 [PMID: 32222543] (WB, Human)	WB	Human
Teixeira M, Gouveia M, Duarte A et al. Regular exercise participation contributes to better proteostasis, inflammatory and vasoactive profiles in patients with hypertension Am. J. Hypertens. [PMID: 31560738]
Samad HA, Konyak YY, Latheef SK et al. Alpha lipoic acid supplementation ameliorates the wrath of simulated tropical heat and humidity stress in male Murrah buffaloes Int J Biometeorol 2019-07-06 [PMID: 31280374]
Verma N, Pink M, Rettenmeier AW, Schmitz-Spanke S. Benzo[a]pyrene-mediated toxicity in primary pig bladder epithelial cells: A proteomic approach. J Proteomics 2013-04-25 [PMID: 23624345] (WB, Porcine)	WB	Porcine
Pearce SC, Gabler NK, Ross JW. The effects of heat stress and plane of nutrition on metabolism in growing pigs. J Anim Sci 2013-05-01 [PMID: 23463563]

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	WB	Human	05/14/2014	View Summary Application Western Blot Sample Tested Hela whole cell lysate Species Human

Array NB110-61582

• HSP70/HSPA1A Antibodies
• HSP70/HSPA1A Lysate
• HSP70/HSPA1A Proteins
• HSP70/HSPA1A ELISAs
• HSP70/HSPA1A Proteins and Enzyme