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Glucose 6 Phosphate Dehydrogenase Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Analysis in human testis and skeletal muscle tissues. Corresponding G6PD RNA-seq data are presented ...read more
Orthogonal Strategies: Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Analysis in human cell lines A-549 and HEK293 using anti-G6PD antibody. Corresponding G6PD RNA-seq data are presented ...read more
Independent Antibodies: Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Analysis using Anti-G6PD antibody NBP1-89804 (A) shows similar pattern to independent antibody NBP1-89805 (B).
Immunocytochemistry/ Immunofluorescence: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Staining of human cell line U-2 OS shows positivity in cytoplasm. Antibody staining is shown in green. Antibody staining ...read more
Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Staining of human spleen shows moderate cytoplasmic positivity in lymphoid cells.
Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Staining of human colon shows moderate cytoplasmic positivity in a subset of lymphoid cells.
Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Staining of human liver shows moderate to strong cytoplasmic positivity in Kupffer cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Glucose 6 Phosphate Dehydrogenase Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FQGDAFHQSDTHIFIIMGASGDLAKKKIYPTIWWLFRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKATPEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHMNALH
Marker
Cytosol Marker
Predicted Species
Mouse (92%), Rat (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
G6PD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Glucose 6 Phosphate Dehydrogenase Recombinant Protein Antigen (NBP1-89804PEP)
Publications
Read Publications using NBP1-89804.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Glucose 6 Phosphate Dehydrogenase Antibody

  • G6PD
  • G6PD1EC 1.1.1.49
  • G6PDH
  • glucose-6-phosphate dehydrogenase

Background

Glucose-6-phosphate dehydrogenase (G6PD) is a cytosolic enzyme that catalyzes the conversion of glucose-6-phosphate into 6-phosphgluconolactone and maintains the levels of the electron donor, NADPH. G6PD is an x-linked housekeeping gene whose deficiency can result in neonatal jaundice, acute hemolysis, or chronic non-spherocytic hemolytic anemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glucose 6 Phosphate Dehydrogenase Antibody (NBP1-89804)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol G6PD
Entrez
Uniprot