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Glucose 1-dehydrogenase Antibody (OTI2A7)

Images

 
Western Blot: Glucose 1-dehydrogenase Antibody (2A7) [NBP2-02615] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glucose 1-dehydrogenase (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunocytochemistry/ Immunofluorescence: Glucose 1-dehydrogenase Antibody (2A7) [NBP2-02615] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Glucose 1-dehydrogenase.
Immunohistochemistry-Paraffin: Glucose 1-dehydrogenase Antibody (2A7) [NBP2-02615] - Staining of paraffin-embedded Human prostate tissue using anti-Glucose 1-dehydrogenase mouse monoclonal antibody.
Flow Cytometry: Glucose 1-dehydrogenase Antibody (2A7) [NBP2-02615] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Glucose ...read more
Western Blot: Glucose 1-dehydrogenase Antibody (2A7) [NBP2-02615] Analysis of extracts (35ug) from 9 different cell lines by using anti-Glucose 1-dehydrogenase monoclonal antibody.
Immunohistochemistry-Paraffin: Glucose 1-dehydrogenase Antibody (2A7) [NBP2-02615] - Staining of paraffin-embedded Human colon tissue using anti-Glucose 1-dehydrogenase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Glucose 1-dehydrogenase Antibody (2A7) [NBP2-02615] - Staining of paraffin-embedded Human liver tissue using anti-Glucose 1-dehydrogenase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Glucose 1-dehydrogenase Antibody (2A7) [NBP2-02615] - Staining of paraffin-embedded Human pancreas tissue using anti-Glucose 1-dehydrogenase mouse monoclonal antibody.
Flow Cytometry: Glucose 1-dehydrogenase Antibody (2A7) [NBP2-02615] - Analysis of Jurkat cells, using anti-Glucose 1-dehydrogenase antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI2A7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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Glucose 1-dehydrogenase Antibody (OTI2A7) Summary

Immunogen
Full length human recombinant protein of human H6PD (NP_004276) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
H6PD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:250-500
Theoretical MW
88.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Glucose 1-dehydrogenase Antibody (OTI2A7)

  • 6-phosphogluconolactonase
  • DKFZp686A01246
  • EC 1.1.1.49
  • EC 2.7.4.3
  • EC 3.1.1.31
  • G6PD, H form
  • G6PDH
  • GDH/6PGL endoplasmic bifunctional protein
  • GDHglucose 1- dehydrogenase
  • glucose dehydrogenase
  • glucose dehyrogenase
  • glucose-6-phosphate dehydrogenase, salivary
  • hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)
  • MGC87643

Background

There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form, encoded by this gene, is autosomally linked. This H form shows activity with other hexose-6-phosphates, especially galactose-6-phosphate, whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues, but H form is not found in red cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol H6PD
Entrez