Novus Biologicals products are now on bio-techne.com

Ghrelin/Obestatin Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: Ghrelin/Obestatin Antibody [NBP1-89773] - Analysis in human stomach and liver tissues. Corresponding Ghrelin/Obestatin RNA-seq data are presented for the same ...read more
Western Blot: Ghrelin/Obestatin Antibody [NBP1-89773] - Analysis in control (vector only transfected HEK293T lysate) and GHRL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian ...read more
Immunohistochemistry-Paraffin: Ghrelin/Obestatin Antibody [NBP1-89773] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Ghrelin/Obestatin Antibody [NBP1-89773] - Staining of human stomach shows high expression.
Immunohistochemistry-Paraffin: Ghrelin/Obestatin Antibody [NBP1-89773] - Staining of human colon shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: Ghrelin/Obestatin Antibody [NBP1-89773] - Staining of human duodenum shows weak cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Ghrelin/Obestatin Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GHRL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Ghrelin/Obestatin Protein (NBP1-89773PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (86%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Ghrelin/Obestatin Antibody

  • appetite-regulating hormone
  • ghrelin
  • ghrelin/obestatin preprohormone
  • ghrelin/obestatin prepropeptide
  • Ghrelin/Obestatin
  • Growth hormone secretagogue
  • Growth hormone-releasing peptide
  • Motilin-related peptide
  • MTLRPgrowth hormone secretagogue receptor ligand
  • obestatin
  • Protein M46

Background

Ghrelin is a 28 residue pepetide expressed by stomach cells that is responsible for the hunger sensation. Expression is increased during times of fasting, and decreased after food consumption. Ghrelin is initially derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 and is involved in satiety and decreased food intake. Ghrelin antibodies are useful tools for lipid and metabolism research as well as neuroscience studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB8200
Species: Hu, Mu
Applications: IHC
AF1067
Species: Hu, Mu, Rt
Applications: ELISA, IHC, Neut, WB
NBP1-90927
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-16655
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP3-04850
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
291-G1
Species: Hu
Applications: BA
AF1067
Species: Hu, Mu, Rt
Applications: ELISA, IHC, Neut, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
NB100-1533
Species: Hu, Mu, Po, Rt, Sh
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
NBP2-37447
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
DLP00
Species: Hu
Applications: ELISA
NBP1-46535
Species: Hu, Mu, Rb, Rt, Xp
Applications: ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, WB
AF1445
Species: Mu, Rt
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, Neut, Simple Western, WB
DGB300
Species: Hu
Applications: ELISA
NBP2-93868
Species: Hu, Mu
Applications: IHC, IHC-P, WB
H00005087-M01
Species: Hu, Pm, Mu
Applications: ChIP, ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA, WB
NBP1-80865
Species: Hu
Applications: IHC, IHC-P
NBP2-50037
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB
NBP1-89773
Species: Hu
Applications: WB, IHC

Publications for Ghrelin/Obestatin Antibody (NBP1-89773) (0)

There are no publications for Ghrelin/Obestatin Antibody (NBP1-89773).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Ghrelin/Obestatin Antibody (NBP1-89773) (0)

There are no reviews for Ghrelin/Obestatin Antibody (NBP1-89773). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Ghrelin/Obestatin Antibody (NBP1-89773) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Ghrelin/Obestatin Products

Research Areas for Ghrelin/Obestatin Antibody (NBP1-89773)

Find related products by research area.

Blogs on Ghrelin/Obestatin

There are no specific blogs for Ghrelin/Obestatin, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Ghrelin/Obestatin Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol GHRL