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DDB1 Antibody

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Western Blot: DDB1 Antibody [NBP2-27207] - Analysis of DDB1 using DDB1 antibody. Human liver tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) Hela and 4) Daudi lysate probed with 2 ug/ml of DDB1 ...read more
Flow Cytometry: DDB1 Antibody [NBP2-27207] - Intracellular analysis of DDB1 in human HeLa and mouse RAW cells using DDB1 antibody at 0.2 ug/10^6 cells. Red represents cells with DDB1 antibody, green represents isotype ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe, Pm, Pm, ChHa, Gp, Pm-Or, RMSpecies Glossary
Applications WB, Flow
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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DDB1 Antibody Summary

Immunogen
A portion of amino acids 385-435 from human DDB1 was used as the immunogen for this antibody.
Predicted Species
Chinese Hamster (94%), Rhesus Macaque (100%), Chimpanzee (100%), Orangutan (100%), Guinea Pig (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDB1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.2ug/10^6cells
  • Western Blot 2-4ug/ml~

Reactivity Notes

The amino acid sequence used as immunogen for this antibody is 100% homologous in Crab-eating Macaque, and 89% in Zebrafish, Cichlid fish, 83% in Chicken and Xenopus.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A purified

Alternate Names for DDB1 Antibody

  • damage-specific DNA binding protein 1 (127kD)
  • damage-specific DNA binding protein 1, 127kDa
  • Damage-specific DNA-binding protein 1
  • DDB p127 Subunit
  • DDB1
  • DDBA
  • DNA damage-binding protein 1
  • DNA damage-binding protein a
  • HBV X-associated protein 1
  • UV-damaged DNA-binding factor
  • UV-damaged DNA-binding protein 1
  • UV-DDB 1
  • UV-DDB1
  • XAP1
  • XAP-1
  • Xeroderma pigmentosum group E-complementing protein
  • XPCE
  • XPE
  • XPE-BF
  • XPE-binding factor

Background

DNA Damage Binding protein 1 (DDB1) binds with CUL4 to form an ubiquitin E3 ligase that targets protein substrates for ubiquitin-dependent proteolysis (1, 2). Loss of DDB1 impedes the proteolysis of a grouping of CUL4 substrates at least in response to UV (3, 4) and CUL4 ubiquitinylation (5). DDB1 is highly conserved from yeast to mammals (3, 6). DDB1 specific antibody probes might be useful in identifying CUL4-DDB1 substrates in epigenetics applications and ubiquitin modification dependent protein function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DDB1
Uniprot