Hu,
Mu,
Rt,
Po,
Bv,
Ca,
Eq,
Fe,
Pm,
Pm,
ChHa,
Gp,
Pm-Or,
RMSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe, Pm, Pm, ChHa, Gp, Pm-Or, RM
Applications: WB, Flow
Host: Rabbit Polyclonal
Alt Catalog# IMG-6799A
Sample Size Available
Sizes: Sample Size Available
![Western Blot: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of DDB1 on PC-12 cell lysates with Rabbit anti-DDB1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 127 kDa Observed band size: 127 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Western-Blot-NBP2-75465-img0009.jpg "Western Blot: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of DDB1 on PC-12 cell lysates with Rabbit anti-DDB1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 127 kDa Observed band size: 127 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: DDB1 Antibody (JU32-35) [NBP2-75465] - Staining DDB1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunocytochemistry-Immunofluorescence-NBP2-75465-img0003.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of paraffin-embedded rat esophagus tissue using anti-DDB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunohistochemistry-Paraffin-NBP2-75465-img0007.jpg)
![Western Blot: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of DDB1 on different lysates using anti-DDB1 antibody at 1/500 dilution.Positive control:Lane 1: HepG2 Lane 2: NIH-3T3Lane 3: MCF-7 Lane 4: Rat kidney tissue](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Western-Blot-NBP2-75465-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: DDB1 Antibody (JU32-35) [NBP2-75465] - Staining DDB1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunocytochemistry-Immunofluorescence-NBP2-75465-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: DDB1 Antibody (JU32-35) [NBP2-75465] - Staining DDB1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunocytochemistry-Immunofluorescence-NBP2-75465-img0002.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of paraffin-embedded human liver cancer tissue using anti-DDB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunohistochemistry-Paraffin-NBP2-75465-img0004.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of paraffin-embedded human kidney tissue using anti-DDB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunohistochemistry-Paraffin-NBP2-75465-img0005.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of paraffin-embedded mouse brain tissue using anti-DDB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunohistochemistry-Paraffin-NBP2-75465-img0006.jpg)
![Western Blot: DDB1 Antibody [NBP1-33061] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DDB1-Antibody-Western-Blot-NBP1-33061-img0015.jpg "Western Blot: DDB1 Antibody [NBP1-33061] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: DDB1 Antibody [NBP1-33061] - Analysis of paraformaldehyde-fixed HeLa.](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33061-img0003.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody [NBP1-33061] - Mouse brain. DDB1 stained by DDB1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunohistochemistry-Paraffin-NBP1-33061-img0018.jpg)
![Western Blot: DDB1 Antibody [NBP1-33061] - Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of DDB1 protein by 2. 5 ug of DDB1 antibody 7. 5% SDS-PAGE gel.](http://images.novusbio.com/fullsize/DDB1-Antibody-Western-Blot-NBP1-33061-img0006.jpg)
![Western Blot: DDB1 Antibody [NBP1-33061] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/DDB1-Antibody-Western-Blot-NBP1-33061-img0007.jpg)
![Western Blot: DDB1 Antibody [NBP1-33061] - Sample (30 ug of whole cell lysate) NIH-3T37C 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/DDB1-Antibody-Western-Blot-NBP1-33061-img0008.jpg)
![Western Blot: DDB1 Antibody [NBP1-33061] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/DDB1-Antibody-Western-Blot-NBP1-33061-img0013.jpg)
![Western Blot: DDB1 Antibody [NBP1-33061] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/DDB1-Antibody-Western-Blot-NBP1-33061-img0014.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody [NBP1-33061] - Cal27 Xenograft , using DDB1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunohistochemistry-Paraffin-NBP1-33061-img0005.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody [NBP1-33061] - Mouse prostate. DDB1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunohistochemistry-Paraffin-NBP1-33061-img0011.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody [NBP1-33061] - Rat brain. DDB1 stained by DDB1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunohistochemistry-Paraffin-NBP1-33061-img0017.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: DDB1 Antibody [NBP1-33061] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDB1 antibody ).](http://images.novusbio.com/fullsize/DDB1-Antibody-Western-Blot-NBP1-33061-img0012.jpg)
![Western Blot: DDB1 Antibody [NBP2-27207] - Analysis of DDB1 using DDB1 antibody. Human liver tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) Hela and 4) Daudi lysate probed with 2 ug/ml of DDB1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/DDB1-Antibody-Western-Blot-NBP2-27207-img0001.jpg "Western Blot: DDB1 Antibody [NBP2-27207] - Analysis of DDB1 using DDB1 antibody. Human liver tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) Hela and 4) Daudi lysate probed with 2 ug/ml of DDB1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: DDB1 Antibody [NBP2-27207] - Intracellular analysis of DDB1 in human HeLa and mouse RAW cells using DDB1 antibody at 0.2 ug/10^6 cells. Red represents cells with DDB1 antibody, green represents isotype control.](http://images.novusbio.com/fullsize/DDB1-Antibody-Flow-Cytometry-NBP2-27207-img0002.jpg)
![Western Blot: DDB1 Antibody (9L8X5) [NBP3-16531] - Western blot analysis of extracts of various cell lines, using DDB1 Rabbit mAb (NBP3-16531) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/DDB1-Antibody-9L8X5-Western-Blot-NBP3-16531-img0004.jpg "Western Blot: DDB1 Antibody (9L8X5) [NBP3-16531] - Western blot analysis of extracts of various cell lines, using DDB1 Rabbit mAb (NBP3-16531) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: DDB1 Antibody (9L8X5) [NBP3-16531] - Immunohistochemistry of paraffin-embedded mouse brain using DDB1 Rabbit mAb (NBP3-16531) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DDB1-Antibody-9L8X5-Immunohistochemistry-Paraffin-NBP3-16531-img0003.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody (9L8X5) [NBP3-16531] - Immunohistochemistry of paraffin-embedded rat brain using DDB1 Rabbit mAb (NBP3-16531) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DDB1-Antibody-9L8X5-Immunohistochemistry-Paraffin-NBP3-16531-img0001.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody (9L8X5) [NBP3-16531] - Immunohistochemistry of paraffin-embedded human esophageal using DDB1 Rabbit mAb (NBP3-16531) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DDB1-Antibody-9L8X5-Immunohistochemistry-Paraffin-NBP3-16531-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DDB1 Antibody [NBP2-38538] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38538-img0004.jpg "Immunocytochemistry/Immunofluorescence: DDB1 Antibody [NBP2-38538] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: DDB1 Antibody [NBP2-38538] - Staining of human adrenal gland shows nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunohistochemistry-Paraffin-NBP2-38538-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: DDB1 Antibody [NBP2-98785] - Staining of DDB1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human DDB1 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98785-img0001.jpg "Immunocytochemistry/Immunofluorescence: DDB1 Antibody [NBP2-98785] - Staining of DDB1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human DDB1 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.")
![Immunohistochemistry-Paraffin: DDB1 Antibody [NBP2-98785] - Staining of human DDB1 in human breast with rabbit polyclonal antibody (1:1000).](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunohistochemistry-Paraffin-NBP2-98785-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DDB1 Antibody [NBP2-57877] - Staining of human cell line A549 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57877-img0001.jpg "Immunocytochemistry/Immunofluorescence: DDB1 Antibody [NBP2-57877] - Staining of human cell line A549 shows localization to nucleoplasm.")
![Western Blot: DDB1 Antibody (C.15200221) [NBP2-62791] - Whole cell extracts from human HeLa cells were analyzed using the antibody against DDB1 diluted 1:500 in PBS containing 10% milk. The position of the protein of interest (expected MW 127 kDa) is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/DDB1-Antibody-C-15200221-Western-Blot-NBP2-62791-img0001.jpg "Western Blot: DDB1 Antibody (C.15200221) [NBP2-62791] - Whole cell extracts from human HeLa cells were analyzed using the antibody against DDB1 diluted 1:500 in PBS containing 10% milk. The position of the protein of interest (expected MW 127 kDa) is indicated on the right; the marker (in kDa) is shown on the left.")