![Western Blot: Cullin 4a Antibody [NBP1-78040] - Western Blot of Cullin 4a antibody. Lane 1: Opal Pre-Stained Ladder. Lane 2: human Testis. Lane 3: human skeletal muscle. Load: 35ug lysate per lane. Primary Antibody: Anti-Cullin 4a at 1:500 overnight at 2-8C. Secondary Antibody: Goat anti-rabbit IgG HRP at 1:70,000 at RT for 30 mins. Expect: ~87.6kDa.](http://images.novusbio.com/fullsize/Cullin-4a-Antibody-Western-Blot-NBP1-78040-img0004.jpg "Western Blot: Cullin 4a Antibody [NBP1-78040] - Western Blot of Cullin 4a antibody. Lane 1: Opal Pre-Stained Ladder. Lane 2: human Testis. Lane 3: human skeletal muscle. Load: 35ug lysate per lane. Primary Antibody: Anti-Cullin 4a at 1:500 overnight at 2-8C. Secondary Antibody: Goat anti-rabbit IgG HRP at 1:70,000 at RT for 30 mins. Expect: ~87.6kDa.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | This Cullin 4a Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near amino acids 1-25 of Human Cullin 4a coupled to KLH. (Uniprot: Q13619) |
| Isotype | Serum |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CUL4A |
| Purity | Delipidation and Defibrination |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This product has been tested by western blot, immunoprecipitation, and immunohistochemistry. The antibody immunoprecipitates protein from cell lysates (using HeLa, U2OS and others). This antibody also co-immunoprecipitates at least 8 subunits of COP9, DDB1 and CAND1. An 87.6 kDa band corresponding to human Cul4A is detected. Most cell lines expressing Cul4A can be used as a positive control. Researchers should determine optimal titers for other applications. |
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| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | Antiserum |
| Preservative | 0.01% Sodium Azide |
| Purity | Delipidation and Defibrination |
| Publication using NBP1-78040 | Applications | Species |
|---|---|---|
| Higa LA, Mihaylov IS, Banks DP et al. Radiation-mediated proteolysis of CDT1 by CUL4-ROC1 and CSN complexes constitutes a new checkpoint. Nat Cell Biol 2003-11-01 [PMID: 14578910] |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry: Cullin 4a Antibody [NBP1-78040] - Immunohistochemistry. Cullin 4a antibody was diluted 1:500 to detect Cullin 4a in human testes tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.](http://images.novusbio.com/fullsize/Cullin 4a Antibody-Immunohistochemistry-NBP1-78040-img0005.jpg "Immunohistochemistry: Cullin 4a Antibody [NBP1-78040] - Immunohistochemistry. Cullin 4a antibody was diluted 1:500 to detect Cullin 4a in human testes tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.")
![N/A: Cullin 4a Antibody [NBP1-78040]](http://images.novusbio.com/fullsize/Cullin-4a-Antibody-N-A-NBP1-78040-img0002.jpg "N/A: Cullin 4a Antibody [NBP1-78040]")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")