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RBX1 Antibody (SR1116)

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Western Blot: RBX1 Antibody (SR1116) [NBP3-21835] - Western blot analysis using NBP3-21835 in MCF7 cell lysate.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP
Clone
SR1116
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

RBX1 Antibody (SR1116) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human RBX1 (Uniprot #: P62877)
Specificity
Detects endogenous levels of total RBX1
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
RBX1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
Theoretical MW
12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for RBX1 Antibody (SR1116)

  • BA554C12.1
  • EC 6.3.2.-
  • FLJ60363
  • MGC13357
  • Protein ZYP
  • RBX1
  • Regulator of cullins 1
  • Ring Box 1
  • RING box protein 1
  • RING finger protein 75
  • ring-box 1
  • ring-box 1, E3 ubiquitin protein ligase
  • RING-box protein 1
  • RNF75
  • RNF75MGC1481
  • ROC1E3 ubiquitin-protein ligase RBX1
  • ZYP Protein

Background

ROC1 is a component of the SCF (SKP1-CUL1-F-box protein) and the CBC(VHL) (CUL2-elonging BC-VHL) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. ROC1 appears to recruit the E2 ubiquitination enzyme through the RING-type zinc finger in a manner similar to CDC34, and brings it into close proximity to the substrate. The RING-type zinc finger domain is essential for ubiquitin ligase activity. ROC1 probably also stimulates CDC34 autoubiquitination and promotes the neddylation of CUL1 and probably CUL2. ROC1 has a cytoplasmic and nuclear localization and is widely expressed in most tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RBX1 Antibody (NBP3-21835) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RBX1