DDB1 Antibodies
We offer DDB1 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Sandwich ELISA, Western Blot. Each DDB1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our DDB1 Antibodies can be used in a variety of model species: Bovine, Canine, Equine, Feline, Human, Mouse, Porcine, Primate, Rat. Use the list below to choose the DDB1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our DDB1 polyclonal antibodies and browse our DDB1 monoclonal antibody catalog.
Alternate Names for DDB1 Antibodies
anti-DDB1 antibody, anti-damage-specific DNA binding protein 1 (127kD) antibody, anti-damage-specific DNA binding protein 1, 127kDa antibody, anti-Damage-specific DNA-binding protein 1 antibody, anti-DDB p127 subunit antibody, anti-DDBA antibody, anti-DNA damage-binding protein 1 antibody, anti-DNA damage-binding protein a antibody, anti-HBV X-associated protein 1 antibody, anti-UV-damaged DNA-binding factor antibody, anti-UV-damaged DNA-binding protein 1 antibody, anti-UV-DDB 1 antibody, anti-UV-DDB1 antibody, anti-XAP-1 antibody, anti-XAP1 antibody, anti-Xeroderma pigmentosum group E-complementing protein antibody, anti-XPCE antibody, anti-XPE antibody, anti-XPE-BF antibody, anti-XPE-binding factor antibody
11 Results for "ddb1" in Primary Antibodies
![Western Blot: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of DDB1 on PC-12 cell lysates with Rabbit anti-DDB1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 127 kDa Observed band size: 127 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Western-Blot-NBP2-75465-img0009.jpg "Western Blot: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of DDB1 on PC-12 cell lysates with Rabbit anti-DDB1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 127 kDa Observed band size: 127 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: DDB1 Antibody (JU32-35) [NBP2-75465] - Staining DDB1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunocytochemistry-Immunofluorescence-NBP2-75465-img0003.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of paraffin-embedded rat esophagus tissue using anti-DDB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunohistochemistry-Paraffin-NBP2-75465-img0007.jpg)
![Western Blot: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of DDB1 on different lysates using anti-DDB1 antibody at 1/500 dilution.Positive control:Lane 1: HepG2 Lane 2: NIH-3T3Lane 3: MCF-7 Lane 4: Rat kidney tissue](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Western-Blot-NBP2-75465-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: DDB1 Antibody (JU32-35) [NBP2-75465] - Staining DDB1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunocytochemistry-Immunofluorescence-NBP2-75465-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: DDB1 Antibody (JU32-35) [NBP2-75465] - Staining DDB1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunocytochemistry-Immunofluorescence-NBP2-75465-img0002.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of paraffin-embedded human liver cancer tissue using anti-DDB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunohistochemistry-Paraffin-NBP2-75465-img0004.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of paraffin-embedded human kidney tissue using anti-DDB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunohistochemistry-Paraffin-NBP2-75465-img0005.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of paraffin-embedded mouse brain tissue using anti-DDB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunohistochemistry-Paraffin-NBP2-75465-img0006.jpg)
![Western Blot: DDB1 Antibody [NBP1-33061] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DDB1-Antibody-Western-Blot-NBP1-33061-img0015.jpg "Western Blot: DDB1 Antibody [NBP1-33061] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: DDB1 Antibody [NBP1-33061] - Analysis of paraformaldehyde-fixed HeLa.](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33061-img0003.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody [NBP1-33061] - Mouse brain. DDB1 stained by DDB1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunohistochemistry-Paraffin-NBP1-33061-img0018.jpg)
![Western Blot: DDB1 Antibody [NBP1-33061] - Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of DDB1 protein by 2. 5 ug of DDB1 antibody 7. 5% SDS-PAGE gel.](http://images.novusbio.com/fullsize/DDB1-Antibody-Western-Blot-NBP1-33061-img0006.jpg)
![Western Blot: DDB1 Antibody [NBP1-33061] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/DDB1-Antibody-Western-Blot-NBP1-33061-img0007.jpg)
![Western Blot: DDB1 Antibody [NBP1-33061] - Sample (30 ug of whole cell lysate) NIH-3T37C 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/DDB1-Antibody-Western-Blot-NBP1-33061-img0008.jpg)
![Western Blot: DDB1 Antibody [NBP1-33061] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/DDB1-Antibody-Western-Blot-NBP1-33061-img0013.jpg)
![Western Blot: DDB1 Antibody [NBP1-33061] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/DDB1-Antibody-Western-Blot-NBP1-33061-img0014.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody [NBP1-33061] - Cal27 Xenograft , using DDB1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunohistochemistry-Paraffin-NBP1-33061-img0005.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody [NBP1-33061] - Mouse prostate. DDB1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunohistochemistry-Paraffin-NBP1-33061-img0011.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody [NBP1-33061] - Rat brain. DDB1 stained by DDB1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunohistochemistry-Paraffin-NBP1-33061-img0017.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: DDB1 Antibody [NBP1-33061] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDB1 antibody ).](http://images.novusbio.com/fullsize/DDB1-Antibody-Western-Blot-NBP1-33061-img0012.jpg)
![Western Blot: DDB1 Antibody [NBP2-27207] - Analysis of DDB1 using DDB1 antibody. Human liver tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) Hela and 4) Daudi lysate probed with 2 ug/ml of DDB1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/DDB1-Antibody-Western-Blot-NBP2-27207-img0001.jpg "Western Blot: DDB1 Antibody [NBP2-27207] - Analysis of DDB1 using DDB1 antibody. Human liver tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) Hela and 4) Daudi lysate probed with 2 ug/ml of DDB1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: DDB1 Antibody [NBP2-27207] - Intracellular analysis of DDB1 in human HeLa and mouse RAW cells using DDB1 antibody at 0.2 ug/10^6 cells. Red represents cells with DDB1 antibody, green represents isotype control.](http://images.novusbio.com/fullsize/DDB1-Antibody-Flow-Cytometry-NBP2-27207-img0002.jpg)
![Western Blot: DDB1 Antibody (9L8X5) [NBP3-16531] - Western blot analysis of extracts of various cell lines, using DDB1 Rabbit mAb (NBP3-16531) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/DDB1-Antibody-9L8X5-Western-Blot-NBP3-16531-img0004.jpg "Western Blot: DDB1 Antibody (9L8X5) [NBP3-16531] - Western blot analysis of extracts of various cell lines, using DDB1 Rabbit mAb (NBP3-16531) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: DDB1 Antibody (9L8X5) [NBP3-16531] - Immunohistochemistry of paraffin-embedded mouse brain using DDB1 Rabbit mAb (NBP3-16531) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DDB1-Antibody-9L8X5-Immunohistochemistry-Paraffin-NBP3-16531-img0003.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody (9L8X5) [NBP3-16531] - Immunohistochemistry of paraffin-embedded rat brain using DDB1 Rabbit mAb (NBP3-16531) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DDB1-Antibody-9L8X5-Immunohistochemistry-Paraffin-NBP3-16531-img0001.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody (9L8X5) [NBP3-16531] - Immunohistochemistry of paraffin-embedded human esophageal using DDB1 Rabbit mAb (NBP3-16531) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DDB1-Antibody-9L8X5-Immunohistochemistry-Paraffin-NBP3-16531-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DDB1 Antibody [NBP2-38538] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38538-img0004.jpg "Immunocytochemistry/Immunofluorescence: DDB1 Antibody [NBP2-38538] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: DDB1 Antibody [NBP2-38538] - Staining of human adrenal gland shows nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunohistochemistry-Paraffin-NBP2-38538-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: DDB1 Antibody [NBP2-98785] - Staining of DDB1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human DDB1 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98785-img0001.jpg "Immunocytochemistry/Immunofluorescence: DDB1 Antibody [NBP2-98785] - Staining of DDB1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human DDB1 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.")
![Immunohistochemistry-Paraffin: DDB1 Antibody [NBP2-98785] - Staining of human DDB1 in human breast with rabbit polyclonal antibody (1:1000).](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunohistochemistry-Paraffin-NBP2-98785-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DDB1 Antibody [NBP2-57877] - Staining of human cell line A549 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57877-img0001.jpg "Immunocytochemistry/Immunofluorescence: DDB1 Antibody [NBP2-57877] - Staining of human cell line A549 shows localization to nucleoplasm.")
![Western Blot: DDB1 Antibody (C.15200221) [NBP2-62791] - Whole cell extracts from human HeLa cells were analyzed using the antibody against DDB1 diluted 1:500 in PBS containing 10% milk. The position of the protein of interest (expected MW 127 kDa) is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/DDB1-Antibody-C-15200221-Western-Blot-NBP2-62791-img0001.jpg "Western Blot: DDB1 Antibody (C.15200221) [NBP2-62791] - Whole cell extracts from human HeLa cells were analyzed using the antibody against DDB1 diluted 1:500 in PBS containing 10% milk. The position of the protein of interest (expected MW 127 kDa) is indicated on the right; the marker (in kDa) is shown on the left.")