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XPE/DDB2 Antibody

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human XPE/DDB2 Antigen Affinity-purified Polyclonal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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XPE/DDB2 Antibody Summary

Immunogen
E. coli-derived recombinant human XPE/DDB2
Met1-Lys427
Accession # Q92466
Specificity
Detects human XPE/DDB2 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DDB2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF3297 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for XPE/DDB2 Antibody

  • damage-specific DNA binding protein 2 (48kD)
  • damage-specific DNA binding protein 2, 48kDa
  • Damage-specific DNA-binding protein 2
  • DDB p48 subunit
  • DDB2
  • DDBB
  • DNA damage-binding protein 2
  • FLJ34321
  • UV-damaged DNA-binding protein 2
  • UV-DDB 2
  • UV-DDB2
  • xeroderma pigmentosum group E protein
  • XP5
  • XPE/DDB2

Background

Xeroderma Pigmentosum complementation group E (XPE) patients are defective in a heterodimer of damage-specific DNA-binding activity (DDB1/DDB2). DDB2 is a 48 kDa protein upregulated in cells exposed to ultraviolet light. XP patients exhibit hypersensitivity to ultraviolet light and have a heightened incidence of skin cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for XPE/DDB2 Antibody (AF3297)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC, Western Blot.


Filter By Application
ICC
(1)
Western Blot
(3)
All Applications
Filter By Species
Human
(4)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF3297 Applications Species
Masayuki Kusakabe, Kaoru Sugasawa Fluorescence-microscopy-based assay assessing regulatory mechanisms of global genome nucleotide excision repair in cultured cells STAR Protocols 2023-09-01 [PMID: 37352107]
C Blessing, K Apelt, D van den He, C Gonzalez-L, MB Rother, M van der Wo, R González-P, A Yifrach, A Parnas, RG Shah, TT Kuo, DEC Boer, J Cai, A Kragten, HS Kim, OD Schärer, ACO Vertegaal, GM Shah, S Adar, H Lans, H van Attiku, AG Ladurner, MS Luijsterbu XPC-PARP complexes engage the chromatin remodeler ALC1 to catalyze global genome DNA damage repair Nature Communications, 2022-08-13;13(1):4762. 2022-08-13 [PMID: 35963869] (Western Blot, Human) Western Blot Human
Masayuki Kusakabe, Erina Kakumu, Fumika Kurihara, Kazuki Tsuchida, Takumi Maeda, Haruto Tada et al. Histone deacetylation regulates nucleotide excision repair through an interaction with the XPC protein iScience 2022-04-15 [PMID: 35330687]
W Sakai, M Yuasa-Suna, M Kusakabe, A Kishimoto, T Matsui, Y Kaneko, JI Akagi, N Huyghe, M Ikura, T Ikura, F Hanaoka, M Yokoi, K Sugasawa Functional impacts of the ubiquitin-proteasome system on DNA damage recognition in global genome nucleotide excision repair Sci Rep, 2020-11-12;10(1):19704. 2020-11-12 [PMID: 33184426] (ICC, Human) ICC Human
TC Lai, MC Hu Regulation of liver receptor homologue-1 by DDB2 E3 ligase activity is critical for hepatic glucose metabolism Sci Rep, 2019-03-28;9(1):5304. 2019-03-28 [PMID: 30923324] (Western Blot, Human) Western Blot Human
Q Zhu, S Wei, N Sharma, G Wani, J He, AA Wani Human CRL4DDB2 ubiquitin ligase preferentially regulates post-repair chromatin restoration of H3K56Ac through recruitment of histone chaperon CAF-1 Oncotarget, 2017-10-17;8(61):104525-104542. 2017-10-17 [PMID: 29262658] (Western Blot, Human) Western Blot Human
Ning Zou, Guozhen Xie, Tiantian Cui, Amit Kumar Srivastava, Meihua Qu, Linlin Yang et al. DDB2 increases radioresistance of NSCLC cells by enhancing DNA damage responses Tumor Biology 2016-10-01 [PMID: 27553023]

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Bioinformatics

Gene Symbol DDB2
Uniprot