DDB1 Antibody (JU32-35)

Damage specific DNA binding protein (DDB) functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, purified human DDB protein was injected into XPE cells. The injected DDB protein stimulated DNA repair to normal levels in those strains that lacked the DDB activity but did not stimulate repair in cells from XPE patients that contained the activity. These results provided direct evidence that defective DDB activity causes the repair defect in a subset of XPE patients and establishes a role for this activity in nucleotide excision repair in vivo. It remains for mutation analysis to demonstrate whether the defect in XPE patients is in the DDB1 gene or the DDB2 gene.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Immunoprecipitation.

Showing Publications 1 - 3 of 3.

Publications using NBP2-75465	Applications	Species
Marjolein van Sluis, Qing Yu, Melanie van der Woude, Camila Gonzalo-Hansen, Shannon C. Dealy, Roel C. Janssens, Hedda B. Somsen, Anisha R. Ramadhin, Dick H. W. Dekkers, Hannah Lena Wienecke, Joris J. P. G. Demmers, Anja Raams, Carlota Davó-Martínez, Diana A. Llerena Schiffmacher, Marvin van Toorn, David Häckes, Karen L. Thijssen, Di Zhou, Judith G. Lammers, Alex Pines, Wim Vermeulen, Joris Pothof, Jeroen A. A. Demmers, Debbie L. C. van den Berg, Hannes Lans, Jurgen A. Marteijn Transcription-coupled DNA–protein crosslink repair by CSB and CRL4 CSA -mediated degradation Nature Cell Biology 2024-04-10 [PMID: 38600236]
Pines A, Schiffmacher DL, Lee SH et al. DDA1, a novel factor in transcription-coupled repair, modulates CRL4CSA dynamics at DNA damage-stalled RNA polymerase II Research square 2023-10-12 [PMID: 37886519] (Immunoprecipitation)	Immunoprecipitation
Llerena Schiffmacher DA, Kliza KW, Theil AF et al. Live cell transcription-coupled nucleotide excision repair dynamics revisited DNA repair 2023-10-01 [PMID: 37716192] (Human)		Human

Array NBP2-75465

• DDB1 Antibodies
• DDB1 Proteins
• DDB1 Proteins and Enzyme