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CXCL2/GRO beta/MIP-2/CINC-3 Antibody

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Recombinant Rat CXCL2/CINC‑3 (Catalog # 525‑C3) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL2/CINC‑3 (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Rat CXCL2/CINC‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF525). The ND50 is typically 0.5-2.5 µg/mL.
Recombinant Rat CXCL2/CINC‑3 (Catalog # 525‑C3) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF525
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF525-SP
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CXCL2/GRO beta/MIP-2/CINC-3 Antibody Summary

Immunogen
E. coli-derived recombinant rat CXCL2/GRO beta /MIP‑2/CINC‑3 
Ser32-Asn100
Accession # P30348
Specificity
Detects rat CXCL2/GRO beta /MIP‑2/CINC‑3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross‑reactivity with recombinant rat (rr) CINC-2 alpha is observed, 15% cross-reactivity with rrCINC-2 beta , recombinant human (rh) GRO alpha , rhGRO beta , rhGRO gamma  is observed and 5% cross-reactivity with rrCINC-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CXCL2
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.20 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Neutralization 0.5-2.5 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publication using
AF525 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL2/GRO beta/MIP-2/CINC-3 Antibody

  • chemokine (C-X-C motif) ligand 2
  • CINC-2a
  • CINC3
  • CINC-3
  • C-X-C Motif Chemokine 2
  • CXCL2
  • GRO beta
  • GRO2 oncogene
  • GRO2
  • GROB
  • Gro-beta
  • Growth-regulated protein beta
  • Macrophage inflammatory protein 2-alpha
  • melanoma growth stimulatory activity beta
  • MGSA beta
  • MGSA-b
  • MGSA-beta
  • MIP2
  • MIP-2
  • MIP2A
  • MIP-2a
  • MIP2-alpha
  • SCYB2

Background

Cytokine-induced neutrophil chemoattractant 3 (CINC-3) has been found to be expressed by cytokine-stimulated rat alveolar macrophages and fibroblasts. Based on its protein and DNA sequences, CINC-3 (also known as rat MIP-2, GRO beta and CXCL2) is a member of the alpha (CXC) subfamily of chemokines.

CINC-3 cDNA encodes a 100 amino acid (aa) residue precursor protein with a 31 aa signal peptide that is cleaved to yield a 69 aa mature secreted protein. The protein sequence of rat CINC-3 shares approximately 88% identity with murine MIP-2. Characteristic of ELR containing CXC chemokines, CINC-3 is known to be a potent chemotactic factor for rat neutrophils in vitro and in vivo. On the basis of cross-desensitization results of the various CINC proteins, it has been postulated that rat neutrophils have at least two classes of CINC receptors: a class of CINC-3 specific receptors as well as a second common receptor shared by all CINCs.

  1. Nakagawa, H. et al. (1994) Biochem. J. 301:545.
  2. Huang, S. et al. (1992) Am. J. Pathol. 141:981.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCL2/GRO beta/MIP-2/CINC-3 Antibody (AF525)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: ICC.


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Bioinformatics

Gene Symbol CXCL2
Uniprot