![Western Blot: CXCL13/BLC/BCA-1 Antibody [NBP2-16041] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with BCA1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CXCL13-BLC-BCA-1-Antibody-Western-Blot-NBP2-16041-img0017.jpg "Western Blot: CXCL13/BLC/BCA-1 Antibody [NBP2-16041] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with BCA1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 0.74 mg/ml |
| Immunogen | Synthetic peptide made to a C-terminal region of human CXCL13 (between amino acids 50-109) [UniProt O43927] |
| Predicted Species | Rhesus Macaque (93%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CXCL13 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Theoretical MW | 13 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
|
| Publications |
|
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.74 mg/ml |
| Purity | Immunogen affinity purified |
![Immunocytochemistry CCR7 Antibody (150503) [Unconjugated]](https://images.novusbio.com/images2/CCR7_MAB197_Immunocytochemistry_6580.jpg)
![Immunohistochemistry CCR7 Antibody (150503) [Unconjugated]](https://images.novusbio.com/images2/CCR7_MAB197_Immunohistochemistry_22800.jpg)
![Neutralization CCR7 Antibody (150503) [Unconjugated]](https://images.novusbio.com/images2/CCR7_MAB197_Block_Neutralize_9048.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for CXCL13/BLC/BCA-1 Antibody (NBP2-16041)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: CXCL13/BLC/BCA-1 Antibody [NBP2-16041] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-CXCL13 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CXCL13-BLC-BCA-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16041-img0019.jpg "Immunocytochemistry/Immunofluorescence: CXCL13/BLC/BCA-1 Antibody [NBP2-16041] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-CXCL13 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: CXCL13/BLC/BCA-1 Antibody - BSA Free [NBP2-16041] - Human breast cancer tissue stained for CXCL13/BLC/BCA-1 (green) and counterstained with DAPI (blue). Alexa Fluor 488 version of antibody used (NBP2-16041AF488). Image from verified customer review.](http://images.novusbio.com/fullsize/CXCL13-BLC-BCA-1-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP2-16041-img0020.jpg "Immunohistochemistry-Paraffin: CXCL13/BLC/BCA-1 Antibody - BSA Free [NBP2-16041] - Human breast cancer tissue stained for CXCL13/BLC/BCA-1 (green) and counterstained with DAPI (blue). Alexa Fluor 488 version of antibody used (NBP2-16041AF488). Image from verified customer review.")
![Western Blot: CXCL13/BLC/BCA-1 Antibody [NBP2-16041] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with BCA1 antibody [N3C3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CXCL13-BLC-BCA-1-Antibody-Western-Blot-NBP2-16041-img0015.jpg "Western Blot: CXCL13/BLC/BCA-1 Antibody [NBP2-16041] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with BCA1 antibody [N3C3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: CXCL13/BLC/BCA-1 Antibody [NBP2-16041] - Analysis of a FFPE tissue section of human esophageal carcinoma using 1:300 dilution of CXCL13 antibody (NBP2-16041). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.](http://images.novusbio.com/fullsize/CXCL13-BLC-BCA-1-Antibody-Immunohistochemistry-Paraffin-NBP2-16041-img0018.jpg "Immunohistochemistry-Paraffin: CXCL13/BLC/BCA-1 Antibody [NBP2-16041] - Analysis of a FFPE tissue section of human esophageal carcinoma using 1:300 dilution of CXCL13 antibody (NBP2-16041). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.")
![Flow Cytometry CXCR5 Antibody (51505) [Unconjugated]](https://images.novusbio.com/images2/CXCR5_MAB190_Flow_Cytometry_22006.jpg)
![Immunohistochemistry CXCR5 Antibody (51505) [Unconjugated]](https://images.novusbio.com/images2/CXCR5_MAB190_Flow_Cytometry_18756.jpg)
![Neutralization CXCR5 Antibody (51505) [Unconjugated]](https://images.novusbio.com/images2/CXCR5_MAB190_Block_Neutralize_9046.jpg)
![SDS-PAGE CXCL12/SDF-1 alpha [Unconjugated]](https://images.novusbio.com/images2/CXCL12_SDF-1_alpha_350-NS_308.jpg)
![Bioactivity CXCL12/SDF-1 alpha [Unconjugated]](https://images.novusbio.com/images2/CXCL12SDF1_alpha_350NS_1178.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![SDS-PAGE Lymphotoxin-alpha/TNF-beta [Unconjugated]](https://images.novusbio.com/images2/Lymphotoxin-alpha_211-TBB_CF_695.jpg)
![Bioactivity Lymphotoxin-alpha/TNF-beta [Unconjugated]](https://images.novusbio.com/images2/Lymphotoxin-alpha_211-TBB_CF_697.jpg)
![N/A CXCL10/IP-10/CRG-2 [HRP]](https://images.novusbio.com/images2/DATA_CXCL10_DIP100_ELISA_723.jpg)
![N/A CXCL10/IP-10/CRG-2 [HRP]](https://images.novusbio.com/images2/DATA_CXCL10_DIP100_ELISA_724.jpg)
![N/A CXCL10/IP-10/CRG-2 [HRP]](https://images.novusbio.com/images2/CXCL10_DIP100_ELISA_121.jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![Immunohistochemistry CXCR4 Antibody (44716) [Unconjugated]](https://images.novusbio.com/images2/CXCR4_MAB172_Immunohistochemistry_6955.jpg)
![Neutralization CXCR4 Antibody (44716) [Unconjugated]](https://images.novusbio.com/images2/CXCR4_MAB172_Block_Neutralize_9041.jpg)
![Immunohistochemistry CXCR4 Antibody (44716) [Unconjugated]](https://images.novusbio.com/images2/CXCR4_MAB172_Immunohistochemistry_19917.jpg)
![N/A IL-10 [Biotin]](https://images.novusbio.com/images2/DATA_IL10_DY417_ELISA_2014.jpg)
![N/A CCL5/RANTES [HRP]](https://images.novusbio.com/images2/CCL5_DRN00_ELISA_393.jpg)
![N/A CCL5/RANTES [HRP]](https://images.novusbio.com/images2/DATA_CCL5_DRN00_ELISA_810.jpg)
![N/A CCL5/RANTES [HRP]](https://images.novusbio.com/images2/DATA_CCL5_DRN00_ELISA_811.jpg)
![N/A CCL2/JE/MCP-1 [HRP]](https://images.novusbio.com/images2/CCL2_DCP00_ELISA_67.jpg)
![N/A CCL2/JE/MCP-1 [HRP]](https://images.novusbio.com/images2/DATA_CCL2_DCP00_ELISA_633.jpg)
![N/A CCL2/JE/MCP-1 [HRP]](https://images.novusbio.com/images2/DATA_CCL2_DCP00_ELISA_634.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![Bioactivity GM-CSF [Unconjugated]](https://images.novusbio.com/images/protein/7954-gmcf_recombinant-human-gm-csf-cho-expressed-protein-cf-bioactivity-272020121514.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")