Recombinant Human CCL21/6Ckine Protein

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

CCL21, also known as 6Ckine, TCA-4, SLC, Exodus-2, and A21, is a 12 kDa homeostatic chemokine that plays an important role in adaptive immune responses and inflammation (1). Unlike other CC chemokines, human CCL21 has a 37 amino acid (aa) C-terminal extension which mediates its attachment to carbohydrate structures and extracellular matrix components (2, 3). Mature human CCL21 shares 71% and 66% aa sequence identity with mouse and rat CCL21, respectively (4‑6). Both human and mouse CCL21 signal through the chemokine receptor CCR7, while mouse CCL21 additionally can signal through CXCR3 (7). CCL21 is constitutively presented on initial lymphatic vessels, high endothelial venules (HEV), and lymph node dendritic cells (DC) (8‑10). Immobilized CCL21 promotes the docking of DC to lymphatic vessels and the retention of T cells by lymph node DC, resulting in T cell priming for activation (8, 9). DC interaction with the anchored chemokine can induce CCL21 cleavage and release of an 8 kDa fragment that lacks the C-terminal extension (10). During chronic inflammation or tissue damage, CCL21 is up‑regulated on local vascular endothelial cells, macrophages, T cells, and neurons (11‑14). In these settings, it promotes fibrosis, inflammatory cytokine production, and neuropathic pain (12‑14). The soluble chemokine is elevated in rheumatoid arthritis synovial fluid and in the serum of coronary artery disease patients (11, 13). CCL21 has been shown to exert either angiogenic or angiostatic effects (11, 15, 16). These effects, in combination with the ability of CCL21 to attract immune suppressor cells (Treg and MDSC) to a tumor site can have positive or negative effects on tumor progression (16, 17).

We have publications tested in 2 confirmed species: Human, Virus.

We have publications tested in 4 applications: Binding Assay, Bioassay, CAR-T (Bioassay), ELISA (Standard).

Showing Publications 1 - 10 of 33. Show All 33 Publications. Collapse Publications.

Publications using 366-6C	Applications	Species
Seetharaman, A;Christopher, V;Dhandapani, H;Jayakumar, H;Dhanushkodi, M;Bhaskaran, N;Rajaraman, S;Ranganathan, R;Sunder Singh, S;Vijayakumar, V;Rajamanickam, A;Suri, A;Jagadish, N;Rajkumar, T;Ramanathan, P; Optimization and Validation of a Harmonized Protocol for Generating Therapeutic-Grade Dendritic Cells in a Randomized Phase II Clinical Trial, Using Two Varied Antigenic Sources Vaccines 2024-01-23 [PMID: 38400096] (Bioassay, Human)	Bioassay	Human
Z Su, J Brooks, J Pelker, T Andreyeva, H Sobon, R Gifford, M Powers, J Wang, C Dower, M Hegen, D Messing, AD Sheehan, JJ Brennan Studies with neutralizing antibodies suggest CXCL8-mediated neutrophil activation is independent of C-C motif chemokine receptor-like 2 (CCRL2) ligand binding function PLoS ONE, 2023-01-20;18(1):e0280590. 2023-01-20 [PMID: 36662882] (Bioassay, Human)	Bioassay	Human
EP Brandum, AS Jørgensen, MB Calvo, K Spiess, FC Peterson, Z Yang, BF Volkman, CT Veldkamp, MM Rosenkilde, CK Goth, GM Hjortø Selective Boosting of CCR7-Acting Chemokines; Short Peptides Boost Chemokines with Short Basic Tails, Longer Peptides Boost Chemokines with Long Basic Tails International Journal of Molecular Sciences, 2022-01-26;23(3):. 2022-01-26 [PMID: 35163323] (Bioassay, Human)	Bioassay	Human
T Radaszkiew, M Nosková, K Gömöryová, O Vondálová, KA Radaszkiew, M Picková, R Víchová, T Gybe?, K Kaiser, L Demková, L Ku?erová, T Bárta, D Pot?šil, Z Zdráhal, K Sou?ek, V Bryja RNF43 inhibits WNT5A-driven signaling and suppresses melanoma invasion and resistance to the targeted therapy Elife, 2021-10-27;10(0):. 2021-10-27 [PMID: 34702444] (Bioassay, Human)	Bioassay	Human
K Kurowarabe, M Endo, D Kobayashi, H Hayasaka CXCL12-stimulated lymphocytes produce secondary stimulants that affect the surrounding cell chemotaxis Biochemistry and Biophysics Reports, 2021-09-07;28(0):101128. 2021-09-07 [PMID: 34527817] (Bioassay, Human)	Bioassay	Human
H Dhandapani, H Jayakumar, A Seetharama, SS Singh, S Ganeshraja, N Jagadish, A Suri, R Thangaraja, P Ramanathan Dendritic cells matured with recombinant human sperm associated antigen 9 (rhSPAG9) induce CD4+, CD8+ T cells and activate NK cells: a potential candidate molecule for immunotherapy in cervical cancer Cancer Cell International, 2021-09-07;21(1):473. 2021-09-07 [PMID: 34493268] (Bioassay, Human)	Bioassay	Human
ML Capitano, A Jaiswal, HE Broxmeyer, Y Pride, S Glover, FG Amlashi, A Kirby, G Srinivasan, EA Williamson, D Mais, R Hromas A humanized monoclonal antibody against the endothelial chemokine CCL21 for the diagnosis and treatment of inflammatory bowel disease PLoS ONE, 2021-07-01;16(7):e0252805. 2021-07-01 [PMID: 34197491] (Bioassay, Human)	Bioassay	Human
FY Mao, YP Lv, CJ Hao, YS Teng, YG Liu, P Cheng, SM Yang, W Chen, T Liu, QM Zou, R Xie, JY Xu, Y Zhuang Helicobacter pylori-induced Rev-erb&alpha fosters gastric bacteria colonization by impairing host innate and adaptive defense Cellular and Molecular Gastroenterology and Hepatology, 2021-03-03;0(0):. 2021-03-03 [PMID: 33676046] (Bioassay, Human)	Bioassay	Human
H Zhang, Y Zheng, Y Pan, C Lin, S Wang, Z Yan, L Lu, G Ge, J Li, YA Zeng, J Chen A mutation that blocks integrin alpha4beta7 activation prevents adaptive immune-mediated colitis without increasing susceptibility to innate colitis BMC Biol., 2020-06-10;18(1):64. 2020-06-10 [PMID: 32522281] (Bioassay, Human)	Bioassay	Human
L Li, MW Shirkey, T Zhang, Y Xiong, W Piao, V Saxena, C Paluskievi, YS Lee, N Toney, BM Cerel, Q Li, T Simon, KD Smith, KL Hippen, BR Blazar, R Abdi, JS Bromberg The lymph node stromal laminin alpha 5 shapes alloimmunity J. Clin. Invest., 2020-05-01;0(0):. 2020-05-01 [PMID: 32017712] (Bioassay, Human)	Bioassay	Human
J Cantero-Pé, J Grau-Expós, C Serra-Pein, DA Rosero, L Luque-Ball, A Astorga-Ga, J Castellví, T Sanhueza, G Tapia, B Lloveras, MA Fernández, JG Prado, JM Solé-Seden, A Tarrats, C Lecumberri, L Mañalich-B, C Centeno-Me, V Falcó, MJ Buzon, M Genescà Resident memory T cells are a cellular reservoir for HIV in the cervical mucosa Nat Commun, 2019-10-18;10(1):4739. 2019-10-18 [PMID: 31628331] (Bioassay, Human)	Bioassay	Human
Z Ye, L Zhong, S Zhu, Y Wang, J Zheng, S Wang, J Zhang, R Huang The P-selectin and PSGL-1 axis accelerates atherosclerosis via activation of dendritic cells by the TLR4 signaling pathway Cell Death Dis, 2019-07-01;10(7):507. 2019-07-01 [PMID: 31263109] (Bioassay, Human)	Bioassay	Human
S Caielli, DT Veiga, P Balasubram, S Athale, B Domic, E Murat, R Banchereau, Z Xu, M Chandra, CH Chung, L Walters, J Baisch, T Wright, M Punaro, L Nassi, K Stewart, J Fuller, D Ucar, H Ueno, J Zhou, J Banchereau, V Pascual A CD4+ T cell population expanded in lupus blood provides B cell help through interleukin-10 and succinate Nat. Med., 2018-11-26;0(0):. 2018-11-26 [PMID: 30478422] (Bioassay, Human)	Bioassay	Human
SIA Mohamed, I Jantan, MA Nafiah, MA Seyed, KM Chan Dendritic cells pulsed with generated tumor cell lysate from Phyllanthus amarus Schum. & Thonn. induces anti-tumor immune response BMC Complement Altern Med, 2018-08-06;18(1):232. 2018-08-06 [PMID: 30081891] (Bioassay, Human)	Bioassay	Human
D Kobayashi, M Endo, H Ochi, H Hojo, M Miyasaka, H Hayasaka Regulation of CCR7-dependent cell migration through�CCR7 homodimer formation Sci Rep, 2017-08-17;7(1):8536. 2017-08-17 [PMID: 28819198] (Bioassay, Human)	Bioassay	Human
JMJ Van den Be, ELJM Smits, M Versteven, H De Reu, ZN Berneman, VFI Van Tendel, E Lion Characterization of Interleukin-15-Transpresenting Dendritic Cells for Clinical Use J Immunol Res, 2017-07-13;2017(0):1975902. 2017-07-13 [PMID: 28785596] (Bioassay, Human)	Bioassay	Human
Alison Swaims-Koh Progesterone Levels Associate with a Novel Population of CCR5+CD38+ CD4 T Cells Resident in the Genital Mucosa with Lymphoid Trafficking Potential J Immunol, 2016-05-27;197(1):368-76. 2016-05-27 [PMID: 27233960] (Bioassay, Human)	Bioassay	Human
Yu , Qing, Lou , Xiang-mi, He , Yan Preferential recruitment of Th17 cells to cervical cancer via CCR6-CCL20 pathway. PLoS ONE, 2015-03-13;10(3):e0120855. 2015-03-13 [PMID: 25768730] (Bioassay, Human)	Bioassay	Human
Tutunea-Fatan , Elena, Majumder , Mousumi, Xin , Xiping, Lala , Peeyush The role of CCL21/CCR7 chemokine axis in breast cancer-induced lymphangiogenesis. Mol Cancer, 2015-02-10;14(1):35. 2015-02-10 [PMID: 25744065] (Bioassay, Human)	Bioassay	Human
Till K, Pettitt A, Slupsky J Expression of functional sphingosine-1 phosphate receptor-1 is reduced by B cell receptor signaling and increased by inhibition of PI3 kinase delta but not SYK or BTK in chronic lymphocytic leukemia cells. J Immunol, 2015-01-28;194(5):2439-46. 2015-01-28 [PMID: 25632006] (Bioassay, Human)	Bioassay	Human
Borgman K, van Zanten T, Manzo C, Cabezon R, Cambi A, Benitez-Ribas D, Garcia-Parajo M Priming by chemokines restricts lateral mobility of the adhesion receptor LFA-1 and restores adhesion to ICAM-1 nano-aggregates on human mature dendritic cells. PLoS ONE, 2014-06-19;9(6):e99589. 2014-06-19 [PMID: 24945611] (Bioassay, Human)	Bioassay	Human
Xu Y, Zhang M, Ramos C, Durett A, Liu E, Dakhova O, Liu H, Creighton C, Gee A, Heslop H, Rooney C, Savoldo B, Dotti G Closely related T-memory stem cells correlate with in vivo expansion of CAR.CD19-T cells and are preserved by IL-7 and IL-15. Blood, 2014-04-29;123(24):3750-9. 2014-04-29 [PMID: 24782509] (CAR-T (Bioassay), Human)	CAR-T (Bioassay)	Human
Irino , Tomoyuki, Takeuchi , Hiroya, Matsuda , Sachiko, Saikawa , Yoshiro, Kawakubo , Hirofumi, Wada , Norihito, Takahashi , Tsunehir, Nakamura , Rieko, Fukuda , Kazumasa, Omori , Tai, Kitagawa , Yuko CC-Chemokine receptor CCR7: a key molecule for lymph node metastasis in esophageal squamous cell carcinoma. BMC Cancer, 2014-04-26;14(0):291. 2014-04-26 [PMID: 24766770] (Bioassay, Human)	Bioassay	Human
Da Silva D, Movius C, Raff A, Brand H, Skeate J, Wong M, Kast W Suppression of Langerhans cell activation is conserved amongst human papillomavirus alpha and beta genotypes, but not a micro genotype. Virology, 2014-02-17;452(0):279-86. 2014-02-17 [PMID: 24606705] (Bioassay, Human)	Bioassay	Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)	Bioassay	Human
Berrih-Aknin S, Ruhlmann N, Bismuth J, Cizeron-Clairac G, Zelman E, Shachar I, Dartevelle P, de Rosbo NK, Le Panse R CCL21 overexpressed on lymphatic vessels drives thymic hyperplasia in myasthenia. Ann. Neurol., 2009-10-01;66(4):521-31. 2009-10-01 [PMID: 19847900] (Bioassay, ELISA (Standard), Human)	Bioassay, ELISA (Standard)	Human
Damas JK, Landro L, Fevang B, Heggelund L, Tjonnfjord GE, Floisand Y, Halvorsen B, Froland SS, Aukrust P Homeostatic chemokines CCL19 and CCL21 promote inflammation in human immunodeficiency virus-infected patients with ongoing viral replication. Clin. Exp. Immunol., 2009-09-01;157(3):400-7. 2009-09-01 [PMID: 19664149] (Bioassay, Human)	Bioassay	Human
Ehlin-Henriksson B, Liang W, Cagigi A, Mowafi F, Klein G, Nilsson A Changes in chemokines and chemokine receptor expression on tonsillar B cells upon Epstein-Barr virus infection. Immunology, 2009-08-01;127(4):549-57. 2009-08-01 [PMID: 19604305] (Bioassay, Human)	Bioassay	Human
Cagigi A, Mowafi F, Phuong Dang LV, Tenner-Racz K, Atlas A, Grutzmeier S, Racz P, Chiodi F, Nilsson A Altered expression of the receptor-ligand pair CXCR5/CXCL13 in B cells during chronic HIV-1 infection. Blood, 2008-09-09;112(12):4401-10. 2008-09-09 [PMID: 18780835] (Bioassay, Human)	Bioassay	Human
Hirahara K, Liu L, Clark RA, Yamanaka K, Fuhlbrigge RC, Kupper TS The majority of human peripheral blood CD4+CD25highFoxp3+ regulatory T cells bear functional skin-homing receptors. J. Immunol., 2006-10-01;177(7):4488-94. 2006-10-01 [PMID: 16982885] (Bioassay, Human)	Bioassay	Human
Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O Novel antiviral activity of chemokines. Virology, 2006-04-17;350(2):484-92. 2006-04-17 [PMID: 16603217] (Binding Assay, Bioassay, Virus)	Binding Assay, Bioassay	Virus
Curiel , Tyler J, Coukos , George, Zou , Linhua, Alvarez , Xavier, Cheng , Pui, Mottram , Peter, Evdemon-Hogan , Melina, Conejo-Garcia , Jose R, Zhang , Lin, Burow , Matthew, Zhu , Yun, Wei , Shuang, Kryczek , Ilona, Daniel , Ben, Gordon , Alan, Myers , Leann, Lackner , Andrew, Disis , Mary L, Knutson , Keith L, Chen , Lieping, Zou , Weiping Specific recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts reduced survival. Nat Med, 2004-08-22;10(9):942-9. 2004-08-22 [PMID: 15322536] (Bioassay, Human)	Bioassay	Human
Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells. Immunology, 2004-06-01;112(2):274-89. 2004-06-01 [PMID: 15147571] (Bioassay, Human)	Bioassay	Human
Show All 33 Publications. Collapse Publications.

Array 366-6C

• CCL21/6Ckine Antibodies
• CCL21/6Ckine Lysate
• CCL21/6Ckine Proteins
• CCL21/6Ckine ELISAs
• CCL21/6Ckine Proteins and Enzymes