Name: CXCR5 Antibody (51505) [Unconjugated]
Brand: Novus Biologicals
SKU: MAB190
Rating: 5 (1 reviews)

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	Flow, IHC, B/N, CyTOF-ready, ICC/IF
Clone	51505
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 FAB190U-100UG

Alexa Fluor 405 FAB190V-100UG

Alexa Fluor 488 FAB190G-100UG

Alexa Fluor 532 FAB190X

Alexa Fluor 594 FAB190T-100UG

Alexa Fluor 647 FAB190R-100UG

Alexa Fluor 700 FAB190N-100UG

Alexa Fluor 750 FAB190S-100UG

Allophycocyanin FAB190A-025

Allophycocyanin FAB190A-100

Allophycocyanin/Cy7 FAB190APCCY7

CoraFluor 1 FAB190CL1

DyLight 350 FAB190D

DyLight 405 FAB190E

DyLight 488 FAB190K

DyLight 550 FAB190L

DyLight 594 FAB190M

DyLight 650 FAB190W

DyLight 680 FAB190Y

DyLight 755 FAB190Z

Fluorescein FAB190F-025

Fluorescein FAB190F-100

HRP FAB190H

Janelia Fluor 525 FAB190JF525

Janelia Fluor 549 FAB190I

Janelia Fluor 585 FAB190JF585

Janelia Fluor 635 FAB190JF635

Janelia Fluor 646 FAB190J

Janelia Fluor 669 FAB190JF669

PE/Atto594 FAB190PEATT594

PE/Cy5.5 FAB190PECY55

PE/Cy7 FAB190PECY7

PerCP (Peridinin-chlorophyll Protein Complex) FAB190C-100

PerCP (Peridinin-chlorophyll Protein Complex) FAB190C-025

Phycoerythrin FAB190P-100

Phycoerythrin FAB190P-025

mFluor Violet 450 SE FAB190MFV450

mFluor Violet 500 SE FAB190MFV500

mFluor Violet 610 SE FAB190MFV610

Catalog# & Formulation	Size	Price
Carrier Free MAB190-SP

Carrier Free FAB190G-100UG

Carrier Free FAB190R-100UG

Carrier Free FAB190S-100UG

Carrier Free FAB190T-100UG

Carrier Free FAB190U-100UG

Carrier Free FAB190V-100UG

Carrier Free FAB190A-100

Carrier Free FAB190C-025

Carrier Free FAB190A-025

Carrier Free FAB190P-100

Carrier Free FAB190P-025

Carrier Free FAB190F-100

Carrier Free FAB190F-025

Carrier Free FAB190C-100

Carrier Free FAB190N-100UG

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB190U-100UG
Alexa Fluor 405	FAB190V-100UG
Alexa Fluor 488	FAB190G-100UG
Alexa Fluor 532	FAB190X
Alexa Fluor 594	FAB190T-100UG
Alexa Fluor 647	FAB190R-100UG
Alexa Fluor 700	FAB190N-100UG
Alexa Fluor 750	FAB190S-100UG
Allophycocyanin	FAB190A-025
Allophycocyanin	FAB190A-100
Allophycocyanin/Cy7	FAB190APCCY7
CoraFluor 1	FAB190CL1
DyLight 350	FAB190D
DyLight 405	FAB190E
DyLight 488	FAB190K
DyLight 550	FAB190L
DyLight 594	FAB190M
DyLight 650	FAB190W
DyLight 680	FAB190Y
DyLight 755	FAB190Z
Fluorescein	FAB190F-025
Fluorescein	FAB190F-100
HRP	FAB190H
Janelia Fluor 525	FAB190JF525
Janelia Fluor 549	FAB190I
Janelia Fluor 585	FAB190JF585
Janelia Fluor 635	FAB190JF635
Janelia Fluor 646	FAB190J
Janelia Fluor 669	FAB190JF669
PE/Atto594	FAB190PEATT594
PE/Cy5.5	FAB190PECY55
PE/Cy7	FAB190PECY7
PerCP (Peridinin-chlorophyll Protein Complex)	FAB190C-100
PerCP (Peridinin-chlorophyll Protein Complex)	FAB190C-025
Phycoerythrin	FAB190P-100
Phycoerythrin	FAB190P-025
mFluor Violet 450 SE	FAB190MFV450
mFluor Violet 500 SE	FAB190MFV500
mFluor Violet 610 SE	FAB190MFV610

CXCR5 Antibody (51505) [Unconjugated]

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CXCR5 Antibody (51505) [Unconjugated] Summary

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Alternate Names for CXCR5 Antibody (51505) [Unconjugated]

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Publications for CXCR5 Antibody (MAB190)(39)

Review for CXCR5 Antibody (MAB190) (1) 51

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Additional Information	Clone 51505 was used by HLDA to establish CD designation
Immunogen	NS0 mouse myeloma cell line transfected with human CXCR5 Met1-Phe372 Accession # P32302
Specificity	Stains human CXCR5 transfectants but not the parental cell lines in flow cytometry. Does not cross-react with human CXCR2, CXCR3, or CXCR4 transfectants.
Source	N/A
Isotype	IgG2b
Clonality	Monoclonal
Host	Mouse
Gene	CXCR5
Purity Statement	Protein A or G purified from hybridoma culture supernatant
Endotoxin Note	<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.5 mg/mL in sterile PBS.

Publications using MAB190	Applications	Species
Krenács, L;Krenács, D;Borbényi, Z;Tóth, E;Nagy, A;Piukovics, K;Bagdi, E; Comparison of Follicular Helper T-Cell Markers with the Expression of the Follicular Homing Marker CXCR5 in Peripheral T-Cell Lymphomas-A Reappraisal of Follicular Helper T-Cell Lymphomas International journal of molecular sciences 2023-12-28 [PMID: 38203606] (Immunohistochemistry, Human)	Immunohistochemistry	Human
Hugues Allard-Chamard, Naoki Kaneko, Alice Bertocchi, Na Sun, Julie Boucau, Hsiao-Hsuan Kuo et al. Extrafollicular IgD−CD27−CXCR5−CD11c− DN3 B cells infiltrate inflamed tissues in autoimmune fibrosis and in severe COVID-19 Cell Reports 2023-06-01 [PMID: 37300833]
N Kaneko, H Chen, CA Perugino, T Maehara, R Munemura, S Yokomizo, J Sameshima, TJ Diefenbach, KR Premo, A Chinju, Y Miyahara, M Sakamoto, M Moriyama, JH Stone, S Nakamura, S Pillai Cytotoxic CD8+ T cells may be drivers of tissue destruction in Sjï¿½gren's syndrome Scientific Reports, 2022-09-14;12(1):15427. 2022-09-14 [PMID: 36104369] (IHC, Human)	IHC	Human
Naoki Kaneko, Julie Boucau, Hsiao-Hsuan Kuo, Cory Perugino, Vinay S. Mahajan, Jocelyn R. Farmer et al. Temporal changes in T cell subsets and expansion of cytotoxic CD4+ T cells in the lungs in severe COVID-19 Clinical Immunology 2022-04-01 [PMID: 35364330]
Naoki Kaneko, Hsiao-Hsuan Kuo, Julie Boucau, Jocelyn R. Farmer, Hugues Allard-Chamard, Vinay S. Mahajan et al. Loss of Bcl-6-Expressing T Follicular Helper Cells and Germinal Centers in COVID-19 Cell 2020-10-01 [PMID: 32877699]
A Julià, G Ávila, R Celis, R Sanmartí, J Ramírez, S Marsal, JD Cañete Lower peripheral helper T cell levels in the synovium are associated with a better response to anti-TNF therapy in rheumatoid arthritis Arthritis Res. Ther., 2020-08-25;22(1):196. 2020-08-25 [PMID: 32843099] (IHC, Human)	IHC	Human
Naoki Kaneko, Hsiao-Hsuan Kuo, Julie Boucau, Jocelyn R. Farmer, Hugues Allard-Chamard, Vinay S. Mahajan et al. The Loss of Bcl-6 Expressing T Follicular Helper Cells and the Absence of Germinal Centers in COVID-19 SSRN Electronic Journal 2023-06-28 [PMID: 32742244]
Antonino Cassotta, Philipp Paparoditis, Roger Geiger, Ramgopal R. Mettu, Samuel J. Landry, Alessia Donati et al. Deciphering and predicting CD4+ T cell immunodominance of influenza virus hemagglutinin Journal of Experimental Medicine 2020-10-05 [PMID: 32644114]
F Facciotti, P Larghi, R Bosotti, C Vasco, N Gagliani, C Cordiglier, S Mazzara, V Ranzani, E Rottoli, S Curti, A Penatti, B Karnani, Y Kobayashi, M Crosti, M Bombaci, JP van Hambur, G Rossetti, R Gualtierot, M Gerosa, S Gatti, S Torretta, L Pignataro, SW Tas, S Abrignani, M Pagani, F Grassi, PL Meroni, RA Flavell, J Geginat Evidence for a pathogenic role of extrafollicular, IL-10-producing CCR6+B helper T cells in systemic lupus erythematosus Proc. Natl. Acad. Sci. U.S.A., 2020-03-17;117(13):7305-7316. 2020-03-17 [PMID: 32184325] (Flow Cytometry, Human)	Flow Cytometry	Human
Takashi Maehara, Naoki Kaneko, Cory A. Perugino, Hamid Mattoo, Jesper Kers,, Hugues Allard-Chamard et al. Cytotoxic CD4+ T lymphocytes may induce endothelial cell apoptosis in systemic sclerosis Journal of Clinical Investigation 2020-04-06 [PMID: 31990684]
Guan-Da Syu, Shih-Chin Wang, Guangzhong Ma, Shuang Liu, Donna Pearce, Atish Prakash et al. Development and application of a high-content virion display human GPCR array Nature Communications 2019-04-30 [PMID: 31040288]
Takashi Maehara, Hamid Mattoo, Vinay S Mahajan, Samuel JH Murphy, Grace J Yuen, Noriko Ishiguro et al. The expansion in lymphoid organs of IL-4+ BATF+ T follicular helper cells is linked to IgG4 class switching in vivo Life Science Alliance 2018-07-10 [PMID: 29984361]
E Armas-Gonz, MJ Domínguez-, A Díaz-Martí, M Arce-Franc, J Castro-Her, G Danelon, V Hernández-, S Bustabad-R, A Cantabrana, M Uguccioni, F Díaz-Gonzá Role of CXCL13 and CCL20 in the recruitment of B cells to inflammatory foci in chronic arthritis Arthritis Res. Ther., 2018-06-07;20(1):114. 2018-06-07 [PMID: 29880013] (Neutralization, Human)	Neutralization	Human
LA Aqrawi, M Ivanchenko, A Björk, JI Ramírez Se, J Imgenberg-, M Kvarnström, P Haselmayer, JL Jensen, G Nordmark, K Chemin, K Skarstein, M Wahren-Her Diminished CXCR5 expression in peripheral blood of patients with Sjï¿½gren's syndrome may relate to both genotype and salivary gland homing Clin. Exp. Immunol., 2018-03-24;0(0):. 2018-03-24 [PMID: 29453859] (IHC-P, Human)	IHC-P	Human
DA Rao, MF Gurish, JL Marshall, K Slowikowsk, CY Fonseka, Y Liu, LT Donlin, LA Henderson, K Wei, F Mizoguchi, NC Teslovich, ME Weinblatt, EM Massarotti, JS Coblyn, SM Helfgott, YC Lee, DJ Todd, VP Bykerk, SM Goodman, AB Pernis, LB Ivashkiv, EW Karlson, PA Nigrovic, A Filer, CD Buckley, JA Lederer, S Raychaudhu, MB Brenner Pathologically expanded peripheral T helper cell subset drives B cells in rheumatoid arthritis Nature, 2017-02-01;542(7639):110-114. 2/1/2017 [PMID: 28150777]
Mitkin N, Hook C, Schwartz A, Biswas S, Kochetkov D, Muratova A, Afanasyeva M, Kravchenko J, Bhattacharyya A, Kuprash D p53-dependent expression of CXCR5 chemokine receptor in MCF-7 breast cancer cells. Sci Rep, 2015-03-19;5(0):9330. 2015-03-19 [PMID: 25786345] (Western Blot, Human)	Western Blot	Human
Chu , Ruiyin, Reczek , David, Brondyk , William Capture-stabilize approach for membrane protein SPR assays. Sci Rep, 2014-12-08;4(0):7360. 2014-12-08 [PMID: 25484112] (Biacore Binding Assay, Human)	Biacore Binding Assay	Human
Rasul E, Salamon D, Nagy N, Leveau B, Banati F, Szenthe K, Koroknai A, Minarovits J, Klein G, Klein E The MEC1 and MEC2 lines represent two CLL subclones in different stages of progression towards prolymphocytic leukemia. PLoS ONE, 2014-08-27;9(8):e106008. 2014-08-27 [PMID: 25162594] (Flow Cytometry, Human)	Flow Cytometry	Human
Humpert M, Pinto D, Jarrossay D, Thelen M CXCR7 influences the migration of B cells during maturation. Eur J Immunol, 2014-02-19;44(3):694-705. 2014-02-19 [PMID: 24259140] (Flow Cytometry, Human)	Flow Cytometry	Human
Mazerolles F, Picard C, Kracker S, Fischer A, Durandy A Blood CD4+CD45RO+CXCR5+ T cells are decreased but partially functional in signal transducer and activator of transcription 3 deficiency. J Allergy Clin Immunol, 2013-02-10;131(4):1146-56, 1156. 2013-02-10 [PMID: 23403044] (Flow Cytometry, Human)	Flow Cytometry	Human
Grant R Stenton, Lloyd F Mackenzie, Patrick Tam, Jennifer L Cross, Curtis Harwig, Jeffrey Raymond et al. Characterization of AQX-1125, a small-molecule SHIP1 activator: Part 1. Effects on inflammatory cell activation and chemotaxis in vitro and pharmacokinetic characterization in vivo British Journal of Pharmacology 2013-03-01 [PMID: 23121445]
Sarra M, Cupi M, Monteleone I, Franze E, Ronchetti G, Di Sabatino A, Gentileschi P, Franceschilli L, Sileri P, Sica G, Del Vecchio Blanco G, Cretella M, Paoluzi O, Corazza G, Pallone F, Monteleone G IL-15 positively regulates IL-21 production in celiac disease mucosa. Mucosal Immunol, 2012-07-11;6(2):244-55. 2012-07-11 [PMID: 22785229] (IHC-Fr, Human)	IHC-Fr	Human
Sun Jin Baek, Sung Keun Kang, Jeong Chan Ra In vitro migration capacity of human adipose tissue-derived mesenchymal stem cells reflects their expression of receptors for chemokines and growth factors Experimental and Molecular Medicine 2011-10-31 [PMID: 21847008]
Islam SA, Chang DS, Colvin RA Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells. Nat. Immunol., 2011-01-09;12(2):167-77. 2011-01-09 [PMID: 21217759] (Flow Cytometry, Human)	Flow Cytometry	Human
Weiss N, Deboux C, Chaverot N, Miller F, Baron-Van Evercooren A, Couraud P, Cazaubon S IL8 and CXCL13 are potent chemokines for the recruitment of human neural precursor cells across brain endothelial cells. J Neuroimmunol, 2010-04-18;223(1):131-4. 2010-04-18 [PMID: 20400187] (Flow Cytometry, Human)	Flow Cytometry	Human
Singh S, Singh R, Sharma PK, Singh UP, Rai SN, Chung LW, Cooper CR, Novakovic KR, Grizzle WE, Lillard JW Serum CXCL13 positively correlates with prostatic disease, prostate-specific antigen and mediates prostate cancer cell invasion, integrin clustering and cell adhesion. Cancer Lett., 2009-04-17;283(1):29-35. 2009-04-17 [PMID: 19375853] (Neutralization, Human)	Neutralization	Human
Panse J, Friedrichs K, Marx A, Hildebrandt Y, Luetkens T, Bartels K, Horn C, Stahl T, Cao Y, Milde-Langosch K, Niendorf A, Kroger N, Wenzel S, Leuwer R, Bokemeyer C, Hegewisch-Becker S, Atanackovic D Chemokine CXCL13 is overexpressed in the tumour tissue and in the peripheral blood of breast cancer patients. Br. J. Cancer, 2008-09-16;99(6):930-8. 2008-09-16 [PMID: 18781150] (IHC-P, Western Blot, Human)	IHC-P, Western Blot	Human
Cagigi A, Mowafi F, Phuong Dang LV, Tenner-Racz K, Atlas A, Grutzmeier S, Racz P, Chiodi F, Nilsson A Altered expression of the receptor-ligand pair CXCR5/CXCL13 in B cells during chronic HIV-1 infection. Blood, 2008-09-09;112(12):4401-10. 2008-09-09 [PMID: 18780835] (IHC-P, Human)	IHC-P	Human
Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells. J. Immunol., 2007-09-15;179(6):3724-33. 2007-09-15 [PMID: 17785809] (Flow Cytometry, Human)	Flow Cytometry	Human
Burkle A, Niedermeier M, Schmitt-Graff A, Wierda WG, Keating MJ, Burger JA Overexpression of the CXCR5 chemokine receptor, and its ligand, CXCL13 in B-cell chronic lymphocytic leukemia. Blood, 2007-07-25;110(9):3316-25. 2007-07-25 [PMID: 17652619] (Neutralization, Human)	Neutralization	Human
Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities. Stem Cells, 2007-03-29;25(7):1737-45. 2007-03-29 [PMID: 17395768] (Flow Cytometry, Human)	Flow Cytometry	Human
Lisignoli G, Cristino S, Piacentini A, Cavallo C, Caplan AI, Facchini A Hyaluronan-based polymer scaffold modulates the expression of inflammatory and degradative factors in mesenchymal stem cells: Involvement of Cd44 and Cd54. J. Cell. Physiol., 2006-05-01;207(2):364-73. 2006-05-01 [PMID: 16331675] (ICC, Human)	ICC	Human
Haas CS, Martinez RJ, Attia N, Haines GK, Campbell PL, Koch AE Chemokine receptor expression in rat adjuvant-induced arthritis. Arthritis Rheum., 2005-12-01;52(12):3718-30. 2005-12-01 [PMID: 16320322] (IHC, Rat)	IHC	Rat
Lim HW, Hillsamer P, Kim CH Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses. J. Clin. Invest., 2004-12-01;114(11):1640-9. 2004-12-01 [PMID: 15578096] (ICC, Human)	ICC	Human
Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. J. Immunol., 2004-12-01;173(11):6592-602. 2004-12-01 [PMID: 15557149] (Flow Cytometry, Human)	Flow Cytometry	Human
Sinha RK, Mage RG Developing neonatal rabbit appendix, a primary lymphoid organ, is seeded by immature blood-borne B cells: evidence for roles for CD62L/PNAd, CCR7/CCL21, alpha4beta1 and LFA-1. Dev. Comp. Immunol., 2004-06-01;28(7):829-41. 2004-06-01 [PMID: 15043950] (Flow Cytometry, Rabbit)	Flow Cytometry	Rabbit
Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, RÃƒÂ©vÃƒÂ©sz T Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL. Blood, 2003-12-04;103(7):2806-8. 2003-12-04 [PMID: 14656893] (Flow Cytometry, Human)	Flow Cytometry	Human
Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H Expression of functional CCR and CXCR chemokine receptors in podocytes. J. Immunol., 2002-06-15;168(12):6244-52. 2002-06-15 [PMID: 12055238] (IHC-Fr, Human)	IHC-Fr	Human
Campbell J, Qin S, Unutmaz D, Soler D, Murphy K, Hodge M, Wu L, Butcher E Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire. J Immunol, 2001-06-01;166(11):6477-82. 2001-06-01 [PMID: 11359797] (Flow Cytometry, Human)	Flow Cytometry	Human
Show All 39 Publications. Collapse Publications.

	Verified Customer 02/12/2015
Application:	WB
Species:	Mouse

Gene Symbol	CXCR5
Uniprot	P32302()