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CENPE Antibody (S06-1G4)

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Western Blot: CENPE Antibody (S06-1G4) [NBP3-19602] - Western blot detection of CENPE in Jurkat lysates using CENPE antibody. Predicted band size: 316kDa. Observed band size: 316kDa.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
S06-1G4
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.3 mg/ml

Order Details

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CENPE Antibody (S06-1G4) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide of human CENPE (Uniprot # Q02224)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CENPE
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1/1000-1/5000
Theoretical MW
316 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
0.3 mg/ml
Purity
Affinity purified

Alternate Names for CENPE Antibody (S06-1G4)

  • CENPE
  • CENP-E
  • Centromere autoantigen E (312kD)
  • centromere protein E (312kD)
  • Centromere protein E
  • centromere protein E, 312kDa
  • centromere-associated protein E
  • KIF10
  • kinesin family member 10
  • Kinesin-7
  • Kinesin-related protein CENPE

Background

Centrosome-associated protein E is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENPE is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Research Areas for CENPE Antibody (NBP3-19602)

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Blogs on CENPE.

CENPF Antibodies as Potential Cancer Markers
Centromere protein F (CENPF), also named mitosin, is a large human protein of 3113 amino acid residues. Its expression and localization are cell cycle-dependent. The protein levels are low in G1 phase but elevated from S to early M phase. CENPF is a n...  Read full blog post.

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Bioinformatics

Gene Symbol CENPE