CD4 Antibody (RPA-T4) - Azide and BSA Free

CD4 (cluster of differentiation 4), also known as L3T4 or T4, is a 55 kDa single chain type I transmembrane glycoprotein belonging to the immunoglobin (Ig) superfamily. CD4 is predominantly expressed on most thymocytes, a subset of mature T lymphocytes, and weakly on monocytes, tissue macrophages, dendritic cells, and granulocytes. It is also expressed on neurons and glial cells in the brain (1). CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost giving rise to single positive (SP) CD4+ or CD8+ mature T cells. CD4+ SP cells (T helper cells) further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity (2). The extracellular region of CD4 consists of 372 amino acids (aa) with four immunoglobin-like domains (D1-D4). The structures of D1 and D3 resemble variable (IgV) domains while D2 and D4 resemble constant (IgC) domains (3).

Given its critical role in T cell development, CD4 also has diverse immunology-related functions. CD4 acts as a coreceptor with the T-cell receptor (TCR) during T cell activation and thymic differentiation by binding directly to major histocompatibility complex (MHC) class II antigens and associating with the protein tyrosine kinase, Lck (4). This interaction contributes to the formation of the immunological synapse (5). Defects in antigen presentation cause dysfunction of CD4+ T cells and the almost complete loss of MHC II expression on B cells in peripheral blood, as observed in severe combined immunodeficiency (SCID) (6). CD4 also functions as a receptor for the human immunodeficiency virus (HIV) by binding to gp120, the envelope glycoprotein of HIV-1. It has been shown that the V-like domains are critical for binding to gp120 (7). In immune mediated and infectious diseases of the central nervous system, CD4 functions as an indirect mediator of neuronal damage (8).

References

1. Omri, B., Crisanti, P., Alliot, F., Marty, M., Rutin, J., Levallois, C., . . . Pessac, B. (1994). CD4 expression in neurons of the central nervous system. International Immunology, 6(3), 377-385. doi:10.1093/intimm/6.3.377

2. Wan, Y. Y., & Flavell, R. A. (2009). How diverse-CD4 effector T cells and their functions. Journal of Molecular Cell Biology, 1(1), 20-36. doi:10.1093/jmcb/mjp001

3. Wu, H., Myszka, D. G., Tendian, S. W., Brouillette, C. G., Sweet, R. W., Chaiken, I. M., & Hendrickson, W. A. (1996). Kinetic and structural analysis of mutant CD4 receptors that are defective in HIV gp120 binding. Proceedings of the National Academy of Sciences, 93(26), 15030-15035. doi:10.1073/pnas.93.26.15030

4. Doyle, C., & Strominger, J. L. (1987). Interaction between CD4 and class II MHC molecules mediates cell adhesion. Nature, 330, 256-259. doi:10.1038/330256a0

5. Vignali, D. A. (2010). CD4 on the road to coreceptor status. The Journal of Immunology, 184(11), 5933-5934. doi:10.4049/jimmunol.1090037

6. Tasher, D., & Dalal, I. (2012). The genetic basis of severe combined immunodeficiency and its variants. The Application of Clinical Genetics, 5, 67-80. doi:10.2147/tacg.s18693

7. Arthos, J., Deen, K. C., Chaikin, M. A., Fornwald, J. A., Sathe, G., Sattentau, Q. J., . . . Sweet, R. W. (1989). Identification of the residues in human CD4 critical for the binding of HIV. Cell, 57(3), 469-481. doi:10.1016/0092-8674(89)90922-7

8. Buttini, M., Westland, C. E., Masliah, E., Yafeh, A. M., Wyss-Coray, T., Mucke, L. (1998). Novel role of human cd4 molecule identified in neurodegeneration. Nature Medicine, 4(4), 441-446. doi:10.1038/nm0498-441

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: FLOW, ICC/IF, IHC-Fr, In vitro.

Showing Publications 1 - 10 of 13. Show All 13 Publications. Collapse Publications.

Publications using NBP2-25199	Applications	Species
Zhang Q, Zong L, Zhang H Et al. B7-H4 Expression in Precancerous Lesions of the Uterine Cervix Biomed Res Int 2021-10-15 [PMID: 34651047] (ICC/IF, Human) Details: Citation using the Azide and BSA Free version of this antibody.	ICC/IF	Human
Hellwinkel JE, Redzic JS, Harland TA et al Glioma-derived extracellular vesicles selectively suppress immune responses. Neuro Oncol. 2016-04-01 [PMID: 26385614] (FLOW, Human) Details: Citation using the Azide Free version of this antibody.	FLOW	Human
Kaneko H, Saito K, Hashimoto H et al. Preferential elimination of CD28+ T cells in systemic lupus erythematosus (SLE) and the relation with activation-induced apoptosis Clin Exp Immunol. 1996-11-01 [PMID: 8918566]
Schols D, De Clercq E. Human immunodeficiency virus type 1 gp120 induces anergy in human peripheral blood lymphocytes by inducing interleukin-10 production J Virol. 1996-08-01 [PMID: 8764000] (In vitro, Human)	In vitro	Human
Res P, Martinez-Caceres E, Cristina Jaleco A et al. CD34+CD38dim cells in the human thymus can differentiate into T, natural killer, and dendritic cells but are distinct from pluripotent stem cells Blood. 1996-06-15 [PMID: 8652833] (FLOW, Human)	FLOW	Human
Ruggiero G, Martinez Caceres E, Voordouw A et al. CD40 expressed on thymic epithelial cells provides costimulation for proliferation but not for apoptosis of human thymocytes J Immunol. 1996-05-15 [PMID: 8621909]
Chabanne L, Marchal T, Kaplanski C et al. Screening of 78 monoclonal antibodies directed against human leukocyte antigens for cross-reactivity with surface markers on canine lymphocytes Tissue Antigens. 1994-03-01 [PMID: 8091419]
Deng MC, Bell S, Huie P et al. Cardiac allograft vascular disease Relationship to microvascular cell surface markers and inflammatory cell phenotypes on endomyocardial biopsy Circulation. 1995-03-15 [PMID: 7882470] (IHC-Fr, Human)	IHC-Fr	Human
Barcena A, Muench MO, Galy AH et al. Phenotypic and functional analysis of T-cell precursors in the human fetal liver and thymus: CD7 expression in the early stages of T- and myeloid-cell development Blood. 1993-12-01 [PMID: 7694684] (FLOW, Human)	FLOW	Human
Galy A, Verma S, Barcena A, Spits H. Precursors of CD3+CD4+CD8+ cells in the human thymus are defined by expression of CD34 Delineation of early events in human thymic development J Exp Med. 1993-08-01 [PMID: 7688021] (FLOW, In vitro, Human)	FLOW, In vitro	Human
Aversa G, Waugh JA, Hall BM. A monoclonal antibody (A6) recognizing a unique epitope restricted to CD45RO and RB isoforms of the leukocyte common antigen family identifies functional T cell subsets Cell Immunol. 1994-10-15 [PMID: 7522974] (In vitro, Human)	In vitro	Human
Staal FJ, Res PC, Weijer K, Spits H. Development of retrovirally marked human T progenitor cells into mature thymocytes Int Immunol. 1995-08-01 [PMID: 7495737]
Wilson MR, Crowley S, Odgers GA, Shaw L. Immunofluorescent labeling using covalently linked anti-phycoerythrin antibodies and phycoerythrin polymers Cytometry. 1991-01-06 [PMID: 2065561]
Show All 13 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Sachin Puri	ICC	Human	02/28/2014	View Summary Application Immunocytochemistry Sample Tested Tonsils, Species Human

Array NBP2-25199

• CD4 Antibodies
• CD4 Antibody Pair
• CD4 ELISA Kits
• CD4 Lysate
• CD4 Proteins
• CD4 Proteins and Enzymes