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CD3 Antibody (17A2) [Biotin]

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Mouse splenocytes were stained with (A) Rat Anti-Mouse Biotinylated CD3 Monoclonal Antibody (Catalog # BAM4841) or (B) Rat IgG2B Biotinylated isotype control antibody (Catalog # IC013B) followed by Streptavidin-Phycoerythrin (Catalog # F0040) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A). View our protocol for Staining Membrane-associated Proteins.
Mouse splenocytes were stained with (A) Rat Anti-Mouse Biotinylated CD3 Monoclonal Antibody (Catalog # BAM4841) or (B) Rat IgG2B Biotinylated isotype control antibody (Catalog # IC013B) followed by ...read more
CD3 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD3 Biotinylated Monoclonal Antibody (Catalog # BAM4841) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, IP, ICC/IF
Clone
17A2
Clonality
Monoclonal
Host
Rat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB4841U-100UG
Alexa Fluor 405
FAB4841V-025
Alexa Fluor 488
FAB4841G-025
Alexa Fluor 488
FAB4841G-100
Alexa Fluor 532
FAB4841X
Alexa Fluor 594
FAB4841T-100
Alexa Fluor 594
FAB4841T-025
Alexa Fluor 647
FAB4841R-100UG
Alexa Fluor 700
FAB4841N-100
Alexa Fluor 750
FAB4841S-025
Allophycocyanin
FAB4841A-100
Allophycocyanin
FAB4841A-025
Allophycocyanin/Cy7
FAB4841APCCY7
CoraFluor 1
FAB4841CL1
DyLight 350
FAB4841D
DyLight 405
FAB4841E
DyLight 488
FAB4841K
DyLight 550
FAB4841L
DyLight 594
FAB4841M
DyLight 650
FAB4841W
DyLight 680
FAB4841Y
DyLight 755
FAB4841Z
Fluorescein
FAB4841F-025
Fluorescein
FAB4841F-100
HRP
FAB4841H
Janelia Fluor 525
FAB4841JF525
Janelia Fluor 549
FAB4841I
Janelia Fluor 585
FAB4841JF585
Janelia Fluor 635
FAB4841JF635
Janelia Fluor 646
FAB4841J
Janelia Fluor 669
FAB4841JF669
PE/Atto594
FAB4841PEATT594
PE/Cy5.5
FAB4841PECY55
PE/Cy7
FAB4841PECY7
PerCP (Peridinin-chlorophyll Protein Complex)
FAB4841C-025
PerCP (Peridinin-chlorophyll Protein Complex)
FAB4841C-100
Phycoerythrin
FAB4841P-025
Phycoerythrin
FAB4841P-100
Unconjugated
MAB4841-100
mFluor Violet 450 SE
FAB4841MFV450
mFluor Violet 500 SE
FAB4841MFV500
mFluor Violet 610 SE
FAB4841MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB4841-100
Carrier Free
FAB4841G-025
Carrier Free
MAB4841-500
Carrier Free
MAB4841-100
Carrier Free
FAB4841V-025
Carrier Free
FAB4841S-025
Carrier Free
MAB4841-SP
Carrier Free
FAB4841T-100
Carrier Free
FAB4841T-025
Carrier Free
FAB4841P-025
Carrier Free
FAB4841F-025
Carrier Free
FAB4841R-100UG
Carrier Free
FAB4841C-025
Carrier Free
FAB4841A-025
Carrier Free
FAB4841P-100
Carrier Free
FAB4841N-100
Carrier Free
FAB4841G-100
Carrier Free
FAB4841F-100
Carrier Free
FAB4841C-100
Carrier Free
FAB4841A-100
Carrier Free
FAB4841U-100UG
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CD3 Antibody (17A2) [Biotin] Summary

Immunogen
T cell hybridoma D1
Specificity
Detects mouse CD3. Recognizes mouse T cell receptor-associated CD3 complex that is expressed on thymocytes and mature T cells.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
CD3E
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation
Readout System

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD3 Antibody (17A2) [Biotin]

  • CD_antigen: CD3e
  • CD3 antigen, delta subunit
  • CD3
  • CD3d antigen
  • CD3d antigen, delta polypeptide (TiT3 complex)
  • CD3d molecule, delta (CD3-TCR complex)
  • CD3-DELTA
  • CD3e antigen
  • CD3e antigen, epsilon polypeptide (TiT3 complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3e
  • CD3-epsilon
  • CD3g antigen
  • CD3g antigen, gamma polypeptide (TiT3 complex)
  • CD3g molecule, epsilon (CD3-TCR complex)
  • CD3g molecule, gamma (CD3-TCR complex)
  • CD3G
  • CD3-GAMMA
  • FLJ17620
  • FLJ17664
  • FLJ18683
  • FLJ79544
  • FLJ94613
  • IMD18
  • MGC138597
  • T3DOKT3, delta chain
  • T3E
  • T-cell antigen receptor complex, epsilon subunit of T3
  • T-cell receptor T3 delta chain
  • T-cell surface antigen T3/Leu-4 epsilon chain
  • T-cell surface glycoprotein CD3 delta chain
  • T-cell surface glycoprotein CD3 epsilon chain
  • TCRE

Background

The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.
  1. Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CD3E