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CD3 zeta Antibody (BL-336-1B2)

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Simple Western: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Detection of human CD247/CD3Z by Simple Western. Samples: Whole cell lysate (2.0 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit ...read more
Flow Cytometry: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Jurkat cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody: 1E6 cells were incubated with 0.5 ul of rabbit ...read more
Immunohistochemistry: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Section of human tonsil. Antibody: Rabbit anti-CD247/CD3Z recombinantmonoclonal antibody [BL-336-1B2] used at 1:250. Secondary: HRP-conjugated goat ...read more
Immunohistochemistry-Paraffin: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal [BL-336-1B2].Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunoprecipitation: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Detection of human CD247/CD3Z by WB of immunoprecipitates from Jurkat lysate.Antibodies: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody ...read more
Immunohistochemistry-Paraffin: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Rabbit antiCD247/CD3Z recombinant monoclonal antibody[BL-336-1B2]. (Secondary: HRPconjugated goat anti-rabbit IgG. Substrate:DAB.
Western Blot: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Whole cell lysate (10 ug) from Jurkat, U2OS, SR, HEK293T, and MOLT-4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant ...read more

Product Details

Summary
Reactivity Hu, Mu, Po, ShSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IP
Clone
BL-336-1B2
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CD3 zeta Antibody (BL-336-1B2) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Immunogen sequence between amino acids 150-164 (C-Term)
Predicted Species
Mouse (100%), Porcine (100%), Sheep (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CD247
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 ul per 1 - 10^6 cells
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 20 ul/mg lysate
  • Simple Western
  • Western Blot 1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

Storage
Store at 2 - 8 C / 1 year from date of receipt
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA
Preservative
0.09% Sodium Azide
Purity
Antigen Affinity-purified

Notes

This Product sold under License from Bethyl Laboratories, Inc.

Alternate Names for CD3 zeta Antibody (BL-336-1B2)

  • CD247 antigen
  • CD247 antigen, zeta subunit
  • CD247 molecule
  • CD247
  • CD3 zeta
  • CD3H
  • CD3Q
  • CD3z antigen, zeta polypeptide (TiT3 complex)
  • CD3Z
  • CD3ZT-cell receptor T3 zeta chain
  • IMD25
  • T3Z
  • T-cell antigen receptor complex, zeta subunit of CD3
  • T-cell surface glycoprotein CD3 zeta chain
  • TCR Zeta Chain
  • TCRZCD3-ZETA

Background

T-cell surface glycoprotein CD3 zeta chain is encoded by the gene CD247, also known as CD3Z or CD3 zeta. CD247/CD3 zeta, together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CD247