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CD3 Antibody (17A2) [Fluorescein]

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Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse CD3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4841F) or ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
17A2
Clonality
Monoclonal
Host
Rat
Conjugate
Fluorescein

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CD3 Antibody (17A2) [Fluorescein] Summary

Immunogen
T cell hybridoma D1
Specificity
Reacts with mouse TCR-associated CD3 complex that occurs on thymocytes and mature T cells.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
CD3E
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Reviewed Applications
Read 1 Review rated 3
using
FAB4841F in the following application:

Publications
Read Publications using
FAB4841F in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD3 Antibody (17A2) [Fluorescein]

  • CD_antigen: CD3e
  • CD3 antigen, delta subunit
  • CD3
  • CD3d antigen
  • CD3d antigen, delta polypeptide (TiT3 complex)
  • CD3d molecule, delta (CD3-TCR complex)
  • CD3-DELTA
  • CD3e antigen
  • CD3e antigen, epsilon polypeptide (TiT3 complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3e
  • CD3-epsilon
  • CD3g antigen
  • CD3g antigen, gamma polypeptide (TiT3 complex)
  • CD3g molecule, epsilon (CD3-TCR complex)
  • CD3g molecule, gamma (CD3-TCR complex)
  • CD3G
  • CD3-GAMMA
  • FLJ17620
  • FLJ17664
  • FLJ18683
  • FLJ79544
  • FLJ94613
  • IMD18
  • MGC138597
  • T3DOKT3, delta chain
  • T3E
  • T-cell antigen receptor complex, epsilon subunit of T3
  • T-cell receptor T3 delta chain
  • T-cell surface antigen T3/Leu-4 epsilon chain
  • T-cell surface glycoprotein CD3 delta chain
  • T-cell surface glycoprotein CD3 epsilon chain
  • TCRE

Background

Monoclonal 17A2 was generated by immunizing rats with a T-cell hybridoma (1). This antibody has been shown to react with the epsilon chain of the CD3 complex (1). The CD3 protein complex is expressed on thymocytes and mature T-cells and, therefore, is a useful reagent to monitor T-cell frequencies in tissues.
  1. Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD3 Antibody (FAB4841F)(2)

Review for CD3 Antibody (FAB4841F) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using FAB4841F:
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Flow
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Flow Cytometry CD3 FAB4841F
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3
reviewed by:
Verified Customer
Flow Mouse 01/30/2019
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Summary

ApplicationFlow Cytometry
Sample TestedMurine Splenocytes (C57B6)
SpeciesMouse
LotLGL0614071

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD3 Antibody (FAB4841F) (0)

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Secondary Antibodies

 

Isotype Controls

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The CD4 Antibody: More than Just a Cellular Marker
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Verified Customer
01/30/2019
Application: Flow
Species: Mouse

Bioinformatics

Gene Symbol CD3E