CD3 Antibodies
CD3 (cluster of differentiation 3) is a multi-subunit transmembrane protein that is expressed on the surface of T-cells and forms a complex with the T-cell receptor (TCR). The CD3 molecule consists of four distinct protein chains (CD3-delta, CD3-epsilon, CD3-gamma, and CD3-zeta) which have theoretical molecular weights ranging between 18 – 23 kDa. We offer antibodies to detect CD3 that have been cited in 45+ publications with multiple 5-star reviews. Browse our extensive catalog for monoclonal antibodies and CD3-conjugated antibodies. Validated research applications for CD3 antibodies include CyTOF-ready, Flow Cytometry, ICC, Immunofluorescence, Knockdown-validated IHC, IHC-Frozen, IHC-Paraffin, IP, and Western Blot. Our CD3 antibodies are suitable in multiple model species including Human, Mouse, Rat, Bovine, Canine, and Porcine. Choose the anti-CD3 antibody best suited for your research needs by viewing the technical details, images, references, reviews, and related products. Each CD3 antibody is fully covered by our Guarantee+.
Alternate Names for CD3 Antibodies
anti-CD3 antibody, anti-Cd247 antibody, anti-CD3E antibody, anti-CD3 antigen, delta subunit antibody, anti-CD3-DELTA antibody, anti-CD3-epsilon antibody, anti-CD3-GAMMA antibody, anti-CD3d antigen antibody, anti-CD3d antigen, delta polypeptide (TiT3 complex) antibody, anti-CD3d molecule, delta (CD3-TCR complex) antibody, anti-CD3e antigen antibody, anti-CD3e antigen, epsilon polypeptide (TiT3 complex) antibody, anti-CD3e molecule, epsilon (CD3-TCR complex) antibody, anti-CD3g antigen antibody, anti-CD3g antigen, gamma polypeptide (TiT3 complex) antibody, anti-CD3g molecule, epsilon (CD3-TCR complex) antibody, anti-CD3g molecule, gamma (CD3-TCR complex) antibody, anti-FLJ17620 antibody, anti-FLJ17664 antibody, anti-FLJ18683 antibody, anti-FLJ79544 antibody, anti-FLJ94613 antibody, anti-MGC138597 antibody, anti-T-cell antigen receptor complex, epsilon subunit of T3 antibody, anti-T-cell receptor T3 delta chain antibody, anti-T-cell surface antigen T3/Leu-4 epsilon chain a
26 Results for "cd3" in Primary Antibodies
![Immunohistochemistry-Paraffin: CD3 Antibody (SP7) [NB600-1441] - FFPE human tonsil stained with CD3 antibody.](http://images.novusbio.com/fullsize/CD3-Antibody-SP7-Immunohistochemistry-Paraffin-NB600-1441-img0001.jpg "Immunohistochemistry-Paraffin: CD3 Antibody (SP7) [NB600-1441] - FFPE human tonsil stained with CD3 antibody.")
![Immunohistochemistry-Frozen: CD3 Antibody (SP7) [NB600-1441] - T-cells and microglia in mouse spinal cord with acute EAE. Mouse was perfused with 4% paraformaldehyde. Spinal cord was post-fixed overnight, followed by cryoprotection with 30% sucrose for 24h. Spinal cord sections were treated with antigen-retrieval buffer (pH 6.0) at 60C for 10 min, then cooled down to room temperature (this step should not be omitted). Anti-CD3 antibody was diluted at 1:200. Incubation: room temperature overnight. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD3-Antibody-SP7-Immunohistochemistry-Frozen-NB600-1441-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: CD3 Antibody (SP7) [NB600-1441] - Mouse lung tissue stained against C3aR normal conditions (left) and knockdown conditions (right). IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD3-Antibody-SP7-Immunohistochemistry-Paraffin-NB600-1441-img0003.jpg)
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis using Alexa Fluor (R) 488 conjugate of NBP2-24867. A cell surface stain was performed on hPBMCs with CD8 Alpha antibody (RPA-T8) NBP2-25195 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to PerCP. A co-stain was also performed using CD3 antibody NBP2-24867AF488.](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0009.jpg "Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis using Alexa Fluor (R) 488 conjugate of NBP2-24867. A cell surface stain was performed on hPBMCs with CD8 Alpha antibody (RPA-T8) NBP2-25195 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to PerCP. A co-stain was also performed using CD3 antibody NBP2-24867AF488.")
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis using the Alexa Fluor(R) 700 conjugate of NBP2-24867. Staining of human PBMC. Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0008.jpg)
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis using Azide Free version of NBP2-25186. CD3+ hPMBCs using the APC conjugate of this antibody. A cell surface stain was performed on CD3+ hPBMCs with V alpha 24 J alpha 18 TCR antibody (6B11) NBP2-00267PE (top image) and a matched isotype control](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0005.jpg)
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis of CD3 expression in hPBMC's gated to lymphocyte population at 1 x 10^6 cells/mL. Cells were stained with NBP2-24867PE (orange) conjugate at a 1:500 dilution. Results are shown with mIgG (PE conjugate) isotype control (blue). Image using the PE format of this antibody.](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0013.jpg)
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis of PerCP conjugate of NBP2-25186. A surface stain was performed on Jurkat cells with CD3 (OKT3) antibody NBP2-24867PCP (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0004.jpg)
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis using the Alexa Fluor(R) 488 conjugate of NBP2-24867. Detection of CD3 expression in hPBMc's gated to lymphocyte population at 1 x 10^6 cells/mL. Cells were stained with NBP2-24867AF488 (orange) conjugate at a 1:500 dilution. Results are shown with mIgG2 (AF488 conjugate) isotype control (blue). Image using the Low Endotoxin and Azide Free form of this antibody.](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0011.jpg)
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. Purified Rat anti Canine CD90 detected with Goat anti Rat IgG2a DyLight 649 and Mouse IgG1 FITC isotype control. Figure B. Purified Rat anti Canine CD90 detected with Goat anti Rat IgG2a PE and Mouse anti Canine CD3 FITC.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0009.jpg "Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. Purified Rat anti Canine CD90 detected with Goat anti Rat IgG2a DyLight 649 and Mouse IgG1 FITC isotype control. Figure B. Purified Rat anti Canine CD90 detected with Goat anti Rat IgG2a PE and Mouse anti Canine CD3 FITC.")
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A: APC conjugated rat anti-Canine CD4 and FITC conjugated mouse IgG1 isotype control. Figure B: APC conjugated rat anti-Canine CD4 and FITC conjugated mouse anti-Canine CD3.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0001.jpg)
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A: RPE conjugated Rat anti-Dog CD4 and Mouse IgG1 isotype control labeled with Goat anti-Mouse FITC. Figure B: RPE conjugated Rat anti-Dog CD4 and Mouse anti-Dog CD3 labeled with Goat anti Mouse DyLight FITC.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0002.jpg)
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. Alexa Fluor 700 conjugated Rat anti Canine CD8 and FITC conjugated Mouse IgG1 isotype control. Figure B. Alexa Fluor 700 conjugated Rat anti Canine CD8 and FITC conjugated Mouse anti Canine CD3.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0003.jpg)
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat IgG2a isotype control. Figure B. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat anti Canine CD4.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0004.jpg)
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. RPE conjugated Rat anti Canine CD4 and FITC conjugated Mouse IgG1 isotype control. Figure B. RPE conjugated Rat anti Canine CD4 and FITC conjugated Mouse anti Canine CD3.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0005.jpg)
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. RPE conjugated Rat anti Canine CD8 and FITC conjugated Mouse IgG1 isotype control. Figure B. RPE conjugated Rat anti Canine CD8 and FITC conjugated Mouse anti Canine CD3.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0006.jpg)
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat IgG1 isotype control. Figure B. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat anti Canine CD8.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0007.jpg)
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. FITC conjugated Mouse anti Canine CD3 and purified Rat IgG2a isotype control detected with Goat anti Rat IgG2a DyLight 649. Figure B. FITC conjugated Mouse anti Canine CD3 and purified Mouse anti Canine CD90 detected with Goat anti Rat IgG2a DyLight 649.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CD3 Antibody (13) [NBP2-43674] - Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD3 protein stained by CD3 antibody [0013] diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/CD3-Antibody-13-Immunocytochemistry-Immunofluorescence-NBP2-43674-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD3 Antibody (13) [NBP2-43674] - Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD3 protein stained by CD3 antibody [0013] diluted at 1:200. Blue: Hoechst 33342 staining.")
![Flow Cytometry: CD3 Antibody (13) [NBP2-43674] - An intracellular stain was performed on Jurkat cells with CD3 Antibody (13) NBP2-43674F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/CD3-Antibody-13-Flow-Cytometry-NBP2-43674-img0004.jpg)
![Flow Cytometry: CD3 Antibody (13) [NBP2-43674] - Analysis of B6 / spleen. Blue: Isotype control (anti-mouse IgG-FITC) dilution: 1:300. Red: CD3 antibody dilution: 1:50.](http://images.novusbio.com/fullsize/CD3-Antibody-13-Flow-Cytometry-NBP2-43674-img0001.jpg)
![Flow Cytometry: CD3 Antibody (13) [NBP2-43674] - Analysis of Jurkat cell Black: Isotype control dilution: 1:50. Green: CD3 antibody dilution: 1:50.](http://images.novusbio.com/fullsize/CD3-Antibody-13-Flow-Cytometry-NBP2-43674-img0002.jpg)
![Flow Cytometry: CD3 Antibody (KT3) [NB100-64871] - Analysis using the PE conjugate of NB100-64871. Staining of mouse spleen cells.](http://images.novusbio.com/fullsize/CD3-Antibody-KT3-Flow-Cytometry-NB100-64871-img0006.jpg "Flow Cytometry: CD3 Antibody (KT3) [NB100-64871] - Analysis using the PE conjugate of NB100-64871. Staining of mouse spleen cells.")
![Flow Cytometry: CD3 Antibody (KT3) [NB100-64871] - Staining of mouse spleen cells using CD3 APC antibody.](http://images.novusbio.com/fullsize/CD3-Antibody-KT3-Flow-Cytometry-NB100-64871-img0001.jpg)
![Flow Cytometry: CD3 Antibody (KT3) [NB100-64871] - Staining of mouse spleen cells using CD3 BIOTIN antibody.](http://images.novusbio.com/fullsize/CD3-Antibody-KT3-Flow-Cytometry-NB100-64871-img0002.jpg)
![Flow Cytometry: CD3 Antibody (KT3) [NB100-64871] - Analysis using the FITC conjugate of NB100-64871. Staining of mouse peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/CD3-Antibody-KT3-Flow-Cytometry-NB100-64871-img0005.jpg)
![Immunohistochemistry-Paraffin: CD3 Antibody (RM0027-3B19) [NBP2-12159] - Mouse kidney section.](http://images.novusbio.com/fullsize/CD3-Antibody-RM0027-3B19-Immunohistochemistry-Paraffin-NBP2-12159-img0002.jpg "Immunohistochemistry-Paraffin: CD3 Antibody (RM0027-3B19) [NBP2-12159] - Mouse kidney section.")
![Immunohistochemistry-Paraffin: CD3 Antibody (RM0027-3B19) [NBP2-12159] - Mouse spleen section.](http://images.novusbio.com/fullsize/CD3-Antibody-RM0027-3B19-Immunohistochemistry-Paraffin-NBP2-12159-img0001.jpg)
![Flow Cytometry: CD3 Antibody (G4.18) [NBP1-43458] - Analysis using the Biotin conjugate of NBP1-43458. Staining of rat splenocytes with anti-Rat CD45R (B220) FITC and staining buffer (autofluorescence) (left) or 0.125 ug of anti-Rat CD3 Biotin (right) followed by Streptavidin PE.](http://images.novusbio.com/fullsize/CD3-Antibody-G4-18-Flow-Cytometry-NBP1-43458-img0003.jpg "Flow Cytometry: CD3 Antibody (G4.18) [NBP1-43458] - Analysis using the Biotin conjugate of NBP1-43458. Staining of rat splenocytes with anti-Rat CD45R (B220) FITC and staining buffer (autofluorescence) (left) or 0.125 ug of anti-Rat CD3 Biotin (right) followed by Streptavidin PE.")
![Flow Cytometry: CD3 Antibody (G4.18) [NBP1-43458] - Staining of rat splenocytes with 0.25 ug of Mouse IgG3 Isotype control (open histogram) or 0.25 ug of Anti-Rat CD3 (filled histogram) followed by anti-Mouse IgG Biotin and Streptavidin PE.](http://images.novusbio.com/fullsize/CD3-Antibody-G4-18-Flow-Cytometry-NBP1-43458-img0001.jpg)
![Flow Cytometry: CD3 Antibody (SK7) [NBP3-09140] - Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (SK7) purified antibody (concentration in sample 4 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/CD3-Antibody-SK7-Flow-Cytometry-NBP3-09140-img0002.jpg "Flow Cytometry: CD3 Antibody (SK7) [NBP3-09140] - Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (SK7) purified antibody (concentration in sample 4 ug/ml) GAM APC.")
![Flow Cytometry: CD3 Antibody (SK7) [NBP3-09140] - Surface staining pattern of human peripheral whole blood stained using anti-human CD3 (SK7) purified antibody (concentration in sample 4 ug/ml) GAM APC](http://images.novusbio.com/fullsize/CD3-Antibody-SK7-Flow-Cytometry-NBP3-09140-img0001.jpg)