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CD3 delta Antibody (2R1O10)

Images

 
Flow Cytometry: CD3 delta Antibody (2R1O10) [NBP3-16881] - 1X10^6 Raji cells (negative control,left) and Jurkat cells (right) were surface-stained with CD3D Rabbit mAb (2.5 μg/mL orange line) or ABflo® 488 IgG ...read more
Western Blot: CD3 delta Antibody (2R1O10) [NBP3-16881] -Western blot analysis of extracts of Jurkat cells, using CD3 delta Rabbit mAb (NBP3-16881) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at ...read more
Immunohistochemistry-Paraffin: CD3 delta Antibody (2R1O10) [NBP3-16881] -Confocal imaging of paraffin-embedded Human spleen tissue using CD3D Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
2R1O10
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

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CD3 delta Antibody (2R1O10) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 72-171 of human CD3 delta (P04234). RCNGTDIYKDKESTVQVHYRMCQSCVELDPATVAGIIVTDVIATLLLALGVFCFAGHETGRLSGAADTQALLRNDQVYQPLRDRDDAQYSHLGGNWARNK
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CD3D
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • Flow Cytometry 1:50 - 1:200
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200 - 1:800
  • Western Blot 1:500 - 1:1000
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for CD3 delta Antibody (2R1O10)

  • CD3 antigen, delta subunit
  • CD3 delta
  • CD3d antigen
  • CD3d antigen, delta polypeptide (TiT3 complex)
  • CD3d molecule, delta (CD3-TCR complex)
  • CD3D
  • CD3-DELTA
  • T3DOKT3, delta chain
  • T-cell receptor T3 delta chain
  • T-cell surface glycoprotein CD3 delta chain

Background

CD3 delta is encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CD3D