CCL5/RANTES Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8 kDa beta -chemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes (1-3). Human and mouse RANTES exhibit cross-species activity on human and mouse cells (4). Mature mouse CCL5 shares 100% aa sequence identity with rat CCL5 and 75-88% with canine, cotton rat, feline, and human CCL5 (5). CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5 (6, 7). Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28 (8). In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1 (9). The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M-tropic HIV-1 infection of monocytes (10). Oligomerization of CCL5 on glycosaminoglycans is required for CCR1-mediated leukocyte adhesion and activation as well as CCL5’s interaction with the chemokine CXCL4/PF4 (11-13). The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes (14-16). CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of pro-inflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature (17).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, In vivo assay, Neutralization, Western Blot.

Showing Publications 1 - 10 of 42. Show All 42 Publications. Collapse Publications.

Publications using AF478	Applications	Species
Lian, N;Li, F;Zhou, C;Yin, Y;Kang, Y;Luo, K;Lui, S;Li, T;Lu, P; Protein phosphatase 2Cm-regulated branched-chain amino acid catabolic defect in dorsal root ganglion neurons drives pain sensitization Acta neuropathologica communications 2024-09-10 [PMID: 39256776] (In vivo assay, Mouse)	In vivo assay	Mouse
Dean, I;Lee, CYC;Tuong, ZK;Li, Z;Tibbitt, CA;Willis, C;Gaspal, F;Kennedy, BC;Matei-Rascu, V;Fiancette, R;Nordenvall, C;Lindforss, U;Baker, SM;Stockmann, C;Sexl, V;Hammond, SA;Dovedi, SJ;Mjösberg, J;Hepworth, MR;Carlesso, G;Clatworthy, MR;Withers, DR; Rapid functional impairment of natural killer cells following tumor entry limits anti-tumor immunity Nature communications 2024-01-24 [PMID: 38267402] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
Liu H, Zhang L, Liu Z et al. Galectin-3 as TREM2 upstream factor contributes to lung ischemia-reperfusion injury by regulating macrophage polarization iScience 2023-09-15 [PMID: 37636061]
Andre AB, Rees KP, O'Connor S et al. Single cell analysis reveals satellite cell heterogeneity for proinflammatory chemokine expression Frontiers in cell and developmental biology 2023-03-27 [PMID: 37051469] Details: 1:500 IHC-P dilution
A Vorontsova, TJ Cooper, J Haj-Shomal, M Benguigui, S Levin, B Manobla, R Menachem, M Timaner, Z Raviv, Y Shaked Chemotherapy-induced tumor immunogenicity is mediated in part by megakaryocyte-erythroid progenitors Oncogene, 2023-01-16;0(0):. 2023-01-16 [PMID: 36646904] (Neutralization, Mouse)	Neutralization	Mouse
S Kochumon, A Hasan, F Al-Rashed, S Sindhu, R Thomas, T Jacob, A Al-Sayyar, H Arefanian, A Al Madhoun, E Al-Ozairi, F Alzaid, HA Koistinen, F Al-Mulla, J Tuomilehto, R Ahmad Increased Adipose Tissue Expression of IL-23 Associates with Inflammatory Markers in People with High LDL Cholesterol Cells, 2022-09-29;11(19):. 2022-09-29 [PMID: 36231033] (IHC, Human)	IHC	Human
Hyun-Jung Kim, Woo-Jin Kim, Hye-Rim Shin, Hee-In Yoon, Jae-I Moon, Eunji Lee et al. ROS-induced PADI2 downregulation accelerates cellular senescence via the stimulation of SASP production and NF kappa B activation Cellular and Molecular Life Sciences 2022-03-01 [PMID: 35218410]
S Tang, B Su, T Tao, W Yan, R Zhang, X Qin, J Feng RGMa regulates CCL5 expression via the BMP receptor in experimental autoimmune encephalomyelitis mice and endothelial cells Molecular Medicine Reports, 2022-01-14;25(3):. 2022-01-14 [PMID: 35029290] (IHC, Mouse)	IHC	Mouse
J Wang, Y Zhang, Y Xiao, X Yuan, P Li, X Wang, Y Duan, VL Seewaldt, D Yu Boosting immune surveillance by low-dose PI3K inhibitor facilitates early intervention of breast cancer American journal of cancer research, 2021-05-15;11(5):2005-2024. 2021-05-15 [PMID: 34094666] (Neutralization, Mouse)	Neutralization	Mouse
AN Seyfried, A McCabe, JNP Smith, LM Calvi, KC MacNamara CCR5 maintains macrophages in the bone marrow and drives hematopoietic failure in a mouse model of severe aplastic anemia Leukemia, 2021-03-20;0(0):. 2021-03-20 [PMID: 33744909] (Neutralization, Mouse)	Neutralization	Mouse
Jorge Luis Galeano Niño, Sophie V Pageon, Szun S Tay, Feyza Colakoglu, Daryan Kempe, Jack Hywood et al. Cytotoxic T cells swarm by homotypic chemokine signalling eLife 2020-10-13 [PMID: 33046212]
K Yagura, H Ohtaki, T Tsumuraya, A Sato, K Miyamoto, N Kawada, K Suzuki, M Nakamura, K Kanzaki, K Dohi, M Izumizaki, Y Hiraizumi, K Honda The enhancement of CCL2 and CCL5 by human bone marrow-derived mesenchymal stem/stromal cells might contribute to inflammatory suppression and axonal extension after spinal cord injury PLoS ONE, 2020-03-10;15(3):e0230080. 2020-03-10 [PMID: 32155215] (IHC, Mouse)	IHC	Mouse
M William, LP Leroux, V Chaparro, TE Graber, T Alain, M Jaramillo Translational repression of Ccl5 and Cxcl10 by 4E-BP1 and 4E-BP2 restrains the ability of mouse macrophages to induce migration of activated T�cells Eur. J. Immunol., 2019-05-06;0(0):. 2019-05-06 [PMID: 31032899] (Neutralization, Mouse)	Neutralization	Mouse
Mouton AJ, Ma Y, Rivera Gonzalez OJ et al. Fibroblast polarization over the myocardial infarction time continuum shifts roles from inflammation to angiogenesis. Basic Res. Cardiol. 1/11/2019 [PMID: 30635789]
L Chen, J Gu, Y Qian, M Li, Y Qian, M Xu, J Li, Y Wen, L Xia, J Li, Q Xia, X Kong, H Wu Deletion of C-C Motif Chemokine Ligand 5 Worsens Invariant Natural Killer T-Cell Mediated Hepatitis via Compensatory Up-regulation of CXCR2 Related Chemokine Activity Cell Mol Gastroenterol Hepatol, 2019-01-07;0(0):. 2019-01-07 [PMID: 30630119] (ICC, Mouse)	ICC	Mouse
JP Böttcher, E Bonavita, P Chakravart, H Blees, M Cabeza-Cab, S Sammicheli, NC Rogers, E Sahai, S Zelenay, C Reis E Sou NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control Cell, 2018-02-08;172(5):1022-1037.e14. 2018-02-08 [PMID: 29429633] (Neutralization, Mouse)	Neutralization	Mouse
P Wang, S Zhou, Y Ge, M Lu, Z Liu, R Gong Valproate hampers podocyte acquisition of immune phenotypes via intercepting the GSK3? facilitated NFkB activation Oncotarget, 2017-08-03;8(51):88332-88344. 2017-08-03 [PMID: 29179438] (Mouse)		Mouse
N Suffee, C Le Visage, H Hlawaty, R Aid-Launai, V Vanneaux, J Larghero, O Haddad, O Oudar, N Charnaux, A Sutton Pro-angiogenic effect of RANTES-loaded polysaccharide-based microparticles for a mouse ischemia therapy Sci Rep, 2017-10-16;7(1):13294. 2017-10-16 [PMID: 29038476] (IHC-Fr, Mouse)	IHC-Fr	Mouse
C Wang, S Yeo, MA Haas, JL Guan Autophagy gene FIP200 in neural progenitors non-cell autonomously controls differentiation by regulating microglia J. Cell Biol., 2017-06-20;216(8):2581-2596. 2017-06-20 [PMID: 28634261] (Neutralization, Mouse)	Neutralization	Mouse
VE Thaney, AM O'Neill, MM Hoefer, R Maung, AB Sanchez, M Kaul IFN? Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury Sci Rep, 2017-04-20;7(0):46514. 2017-04-20 [PMID: 28425451] (Neutralization, Mouse, Rat)	Neutralization	Mouse, Rat
YT Chu, SW Wan, YC Chang, CK Lee, BA Wu-Hsieh, R Anderson, YS Lin Antibodies against nonstructural protein 1 protect mice from dengue virus-induced mast cell activation Lab. Invest, 2017-02-27;0(0):. 2017-02-27 [PMID: 28240747] (IHC-P, Mouse)	IHC-P	Mouse
Neutralization of regulated on activation, normal T cell expressed and secreted and eotaxin prevents the loss of dopaminergic neurons in a mouse model of Parkinson's disease J Biol Chem, 2016-05-12;0(0):. 2016-05-12 [PMID: 27226559] (Neutralization, Mouse)	Neutralization	Mouse
Cheng Yang, Dong-Mei Zhang, Zhen-Bo Song, Ya-Qin Hou, Yong-Li Bao, Lu-Guo Sun et al. Protumoral TSP50 Regulates Macrophage Activities and Polarization via Production of TNF-alpha and IL-1 beta , and Activation of the NF-kappa B Signaling Pathway PLOS ONE 2015-12-18 [PMID: 26684869]
Herz J, Johnson K, McGavern D Therapeutic antiviral T cells noncytopathically clear persistently infected microglia after conversion into antigen-presenting cells. J Exp Med, 2015-06-29;212(8):1153-69. 2015-06-29 [PMID: 26122661] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
Karkeni E, Marcotorchino J, Tourniaire F, Astier J, Peiretti F, Darmon P, Landrier J Vitamin D limits chemokine expression in adipocytes and macrophage migration in vitro and in male mice. Endocrinology, 2015-03-02;156(5):1782-93. 2015-03-02 [PMID: 25730105] (Neutralization, Mouse)	Neutralization	Mouse
Cekanaviciute E, Dietrich H, Axtell R, Williams A, Egusquiza R, Wai K, Koshy A, Buckwalter M Astrocytic TGF-beta signaling limits inflammation and reduces neuronal damage during central nervous system Toxoplasma infection. J Immunol, 2014-05-23;193(1):139-49. 2014-05-23 [PMID: 24860191] (IHC, Mouse)	IHC	Mouse
Bo Liu, Zoheb Hassan, Stefan Amisten, Aileen J. King, James E. Bowe, Guo Cai Huang et al. The novel chemokine receptor, G-protein-coupled receptor 75, is expressed by islets and is coupled to stimulation of insulin secretion and improved glucose homeostasis Diabetologia 2013-11-01 [PMID: 23979485]
Himiko Tokami, Tetsuro Ago, Hiroshi Sugimori, Junya Kuroda, Hideto Awano, Kazuo Suzuki et al. RANTES has a potential to play a neuroprotective role in an autocrine/paracrine manner after ischemic stroke Brain Research 2013-06-23 [PMID: 23602964]
Mishra , Pramod K, Teale , Judy M Changes in gene expression of pial vessels of the blood brain barrier during murine neurocysticercosis. PLoS Negl Trop Dis, 2013-03-14;7(3):e2099. 2013-03-14 [PMID: 23516647] (IHC, Mouse)	IHC	Mouse
Kerstin Knoop, Nathalie Schwenk, Patrick Dolp, Michael J. Willhauck, Christoph Zischek, Christian Zach et al. Stromal Targeting of Sodium Iodide Symporter Using Mesenchymal Stem Cells Allows Enhanced Imaging and Therapy of Hepatocellular Carcinoma Human Gene Therapy 2013-03-01 [PMID: 23402366]
Iida , Yasunori, Xu , Baohui, Xuan , Haojun, Glover , Keith J, Tanaka , Hiroki, Hu , Xiaolei, Fujimura , Naoki, Wang , Wei, Schultz , Joshua R, Turner , Court R, Dalman , Ronald L Peptide inhibitor of CXCL4-CCL5 heterodimer formation, MKEY, inhibits experimental aortic aneurysm initiation and progression. Arterioscler Thromb Vasc Biol, 2013-01-03;33(4):718-26. 2013-01-03 [PMID: 23288157] (IHC, Mouse)	IHC	Mouse
Pramod Kumar Mishra, Judy M Teale Transcriptome analysis of the ependymal barrier during murine neurocysticercosis Journal of Neuroinflammation 2012-12-01 [PMID: 22731103]
Claire Arnaud, Pauline C. Beguin, Sylvie Lantuejoul, Jean-Louis Pepin, Christiane Guillermet, Graziano Pelli et al. The Inflammatory Preatherosclerotic Remodeling Induced by Intermittent Hypoxia Is Attenuated by RANTES/CCL5 Inhibition American Journal of Respiratory and Critical Care Medicine 2011-09-15 [PMID: 21680945]
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 2010-02-08;120(3):907-23. 2010-02-08 [PMID: 20197626] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
Laubli H, Spanaus KS, Borsig L Selectin-mediated activation of endothelial cells induces expression of CCL5 and promotes metastasis through recruitment of monocytes. Blood, 2009-09-24;114(20):4583-91. 2009-09-24 [PMID: 19779041] (IHC, Mouse)	IHC	Mouse
Wu Y, Li YY, Matsushima K, Baba T, Mukaida N CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process. J. Immunol., 2008-11-01;181(9):6384-93. 2008-11-01 [PMID: 18941229] (IHC-P, Mouse)	IHC-P	Mouse
Chou SY, Weng JY, Lai HL, Liao F, Sun SH, Tu PH, Dickson DW, Chern Y Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes. J. Neurosci., 2008-03-26;28(13):3277-90. 2008-03-26 [PMID: 18367595] (ICC, Neutralization, Mouse)	ICC, Neutralization	Mouse
Zhao Z, Burkly LC, Campbell S, Schwartz N, Molano A, Choudhury A, Eisenberg RA, Michaelson JS, Putterman C TWEAK/Fn14 interactions are instrumental in the pathogenesis of nephritis in the chronic graft-versus-host model of systemic lupus erythematosus. J. Immunol., 2007-12-01;179(11):7949-58. 2007-12-01 [PMID: 18025243] (IHC-P, Mouse)	IHC-P	Mouse
Bitko V, Musiyenko A, Barik S Viral infection of the lungs through the eye. J. Virol., 2006-10-18;81(2):783-90. 2006-10-18 [PMID: 17050596] (Western Blot, Mouse)	Western Blot	Mouse
Biswas SK, Gangi L, Paul S, Schioppa T, Saccani A, Sironi M, Bottazzi B, Doni A, Vincenzo B, Pasqualini F, Vago L, Nebuloni M, Mantovani A, Sica A A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation). Blood, 2005-11-03;107(5):2112-22. 2005-11-03 [PMID: 16269622] (IHC, Mouse)	IHC	Mouse
Rubina W. Saeed, Santosh Varma, Tina Peng-Nemeroff, Barbara Sherry, David Balakhaneh, Jared Huston et al. Cholinergic stimulation blocks endothelial cell activation and leukocyte recruitment during inflammation The Journal of Experimental Medicine 2005-04-04 [PMID: 15809354]
Cardona AE, Gonzalez PA, Teale JM CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response. Infect. Immun., 2003-05-01;71(5):2634-42. 2003-05-01 [PMID: 12704138] (ICC, Mouse)	ICC	Mouse
Show All 42 Publications. Collapse Publications.

Array AF478

• CCL5/RANTES Antibodies
• CCL5/RANTES Antibody Pair
• CCL5/RANTES ELISA Kit
• CCL5/RANTES Lysate
• CCL5/RANTES Proteins
• CCL5/RANTES ELISAs
• CCL5/RANTES Proteins and Enzymes