Home » CCL17/TARC » CCL17/TARC Antibodies » CCL17/TARC Antibody [Unconjugated]
Novus Biologicals products are now on bio-techne.com

CCL17/TARC Antibody [Unconjugated]

 (12 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Recombinant Mouse CCL17/TARC (Catalog # 529‑TR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL17/TARC (0.02 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL17/TARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF529). The ND50 is typically0.2-0.6 µg/mL.
Recombinant Mouse CCL17/TARC (Catalog # 529‑TR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more
Cd248 disruption reduced infiltration and pro-fibrotic phenotype switching of kidney macrophages during renal fibrosis. (a) Representative images of F4/80 immunostaining for macrophages in control and D14 UUO kidneys. ...read more
Cd248 disruption reduced infiltration and pro-fibrotic phenotype switching of kidney macrophages during renal fibrosis. (a) Representative images of F4/80 immunostaining for macrophages in control and D14 UUO kidneys. ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF529
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF529-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

CCL17/TARC Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse CCL17/TARC
Ala24-Pro93
Accession # Q9WUZ6
Specificity
Detects mouse CCL17/TARC in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human TARC is observed. Neutralizes the biological activity of recombinant mouse TARC, and will also neutralize the biological activity of recombinant human TARC using a 20 fold greater Ig concentration.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL17
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Neutralization 0.2-0.6 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF529 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL17/TARC Antibody [Unconjugated]

  • ABCD-2
  • CC chemokine TARC
  • C-C motif chemokine 17
  • CCL17
  • chemokine (C-C motif) ligand 17
  • MGC138273
  • SCYA17
  • SCYA17MGC138271
  • small inducible cytokine subfamily A (Cys-Cys), member 17
  • Small-inducible cytokine A17
  • T cell-directed CC chemokine
  • TARC
  • TARCA-152E5.3
  • Thymus and activation-regulated chemokine

Background

Human thymus and activation-regulated chemokine (TARC) also known as CCL17, is a novel CC chemokine identified using a signal sequence trap method. Mouse TARC was discovered as a dendritic cell (DC) specific gene by differentiation RNA display. Mouse TARC cDNA encodes a highly basic 93 amino acid (aa) residue precursor protein with a 23 aa residue putative signal peptide that is cleaved to generate the 70 aa residue mature secreted protein. Among CC chemokine family members, TARC has approximately 24 - 29% amino acid sequence identity with RANTES, MIP-1 alpha , MIP-1 beta , MCP-1, MCP-2, MCP-3 and I-309. The gene for human TARC has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Mouse TARC is constitutively expressed in thymic DC, and at a lower level in lymph node DC in the lung. Recombinant TARC has been shown to be chemotactic for T cell lines and antigen-primed T helper cells. In humans, TARC was identified to be a specific functional ligand for CCR-4 and CCR-8, receptors that are selectively expressed on T cells.

  1. Imai, T. et al. (1997) J. Biol. Chem. 272:15036.
  2. Imai, T. et al. (1996) J. Biol. Chem. 271:21514.
  3. Nomiyama, H. et al. (1997) Genomics 40:211.
  4. Lieberam, I. et al. (1999) Eur. J. Immunol. 29:2684.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

CCL22/MDC [HRP]
DMD00
N/A CCL22/MDC [HRP]
N/A CCL22/MDC [HRP]
(31 Publications)
Species: Hu
Applications: ELISA
CCR4 Antibody
(205410) [Unconjugate
MAB1567
Flow Cytometry CCR4 Antibody (205410) [Unconjugated]
(27 Publications)
Species: Hu
Applications: CyTOF-reported, Flow
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
Nocturnin Antibody
NBP2-37856
Immunohistochemistry-Paraffin Nocturnin Antibody
Immunocytochemistry/ Immunofluorescence Nocturnin Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
CNOT6 Antibody
NBP1-86564
Immunohistochemistry-Paraffin CNOT6 Antibody
Immunocytochemistry/ Immunofluorescence CNOT6 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
ADAM11 Antibody -
Azide and BSA Free
NBP2-92621
Immunocytochemistry/ Immunofluorescence ADAM11 Antibody - Azide and BSA Free
Western Blot ADAM11 Antibody - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence ADAM11 Antibody - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(803 Publications)
Species: Hu
Applications: BA
CXCL10/IP-10/CRG-2
[HRP]
DIP100
N/A CXCL10/IP-10/CRG-2 [HRP]
N/A CXCL10/IP-10/CRG-2 [HRP]
N/A CXCL10/IP-10/CRG-2 [HRP]
(151 Publications)
Species: Hu
Applications: ELISA
IL-13 [Biotin]
DY413
N/A IL-13 [Biotin]
(145 Publications)
Species: Mu
Applications: ELISA
CCL11/Eotaxin [HRP]
MME00
N/A CCL11/Eotaxin [HRP]
N/A CCL11/Eotaxin [HRP]
(57 Publications)
Species: Mu
Applications: ELISA
CCL2/JE/MCP-1 [HRP]
DCP00
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
(250 Publications)
Species: Hu
Applications: ELISA
CCL5/RANTES [HRP]
DRN00B
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
(99 Publications)
Species: Hu
Applications: ELISA
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(445 Publications)
Species: Hu
Applications: BA
IL-5 [HRP]
M5000
N/A IL-5 [HRP]
N/A IL-5 [HRP]
(91 Publications)
Species: Mu
Applications: ELISA
CD4 Antibody - BSA
Free
NBP1-19371
Immunohistochemistry-Frozen CD4 Antibody - BSA Free
Immunohistochemistry-Paraffin CD4 Antibody - BSA Free
Flow Cytometry CD4 Antibody - BSA Free
(9 Reviews)
(50 Publications)
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(289 Publications)
Species: Mu
Applications: ELISA
CXCL9/MIG Antibody
[Unconjugated]
AF-492-NA
Neutralization CXCL9/MIG Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence CXCL9/MIG Antibody [Unconjugated]
Western Blot CXCL9/MIG Antibody [Unconjugated]
(31 Publications)
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), Neut
CXCR3 Antibody
(49801) [Unconjugated
MAB160
Flow Cytometry CXCR3 Antibody (49801) [Unconjugated]
Flow Cytometry CXCR3 Antibody (49801) [Unconjugated]
Neutralization CXCR3 Antibody (49801) [Unconjugated]
(71 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut
CCL27/CTACK [HRP]
DCC270
N/A CCL27/CTACK [HRP]
N/A CCL27/CTACK [HRP]
(10 Publications)
Species: Hu
Applications: ELISA
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(636 Publications)
Species: Mu
Applications: ELISA

Publications for CCL17/TARC Antibody (AF529)(12)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 6 applications: Confocal Microscopy, Flow Cytometry, ICC, In Vivo, Neutralization, Western Blot.


Filter By Application
Confocal Microscopy
(1)
Flow Cytometry
(1)
ICC
(1)
In Vivo
(1)
Neutralization
(8)
Western Blot
(1)
All Applications
Filter By Species
Mouse
(11)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AF529 Applications Species
D Thumkeo, S Punyawatth, S Prasongtan, R Matsuura, K Arima, H Nie, R Yamamoto, N Aoyama, H Hamaguchi, S Sugahara, S Takeda, V Charoensaw, A Tanaka, S Sakaguchi, S Narumiya PGE2-EP2/EP4 signaling elicits immunosuppression by driving the mregDC-Treg axis in inflammatory tumor microenvironment Cell Reports, 2022-06-07;39(10):110914. 2022-06-07 [PMID: 35675777] (In Vivo, Mouse) In Vivo Mouse
Cherng-Shyang Chang, Wen-Hsuan Yu, Chang-Chao Su, Jhen-Wei Ruan, Chiao-Mei Lin, Chih-Ting Huang et al. Single-cell RNA sequencing uncovers the individual alteration of intestinal mucosal immunocytes in Dusp6 knockout mice iScience 2022-02-18 [PMID: 35128351]
KE Chen, M Ghosh, L Rivera, S Lin, A Kumar, S Swaminatha, MY Lorenson, AM Walker Prolactin enhances T regulatory cell promotion of breast cancer through the long form prolactin receptor Translational Oncology, 2021-08-07;14(11):101195. 2021-08-07 [PMID: 34375938] (Neutralization, Mouse) Neutralization Mouse
CH Pai, SR Lin, CH Liu, SY Pan, H Hsu, YT Chen, CT Yen, IS Yu, HL Wu, SL Lin, SW Lin Targeting fibroblast CD248 attenuates CCL17-expressing macrophages and tissue fibrosis Sci Rep, 2020-10-08;10(1):16772. 2020-10-08 [PMID: 33033277] (ICC, Neutralization, Mouse) ICC, Neutralization Mouse
M Valente, Y Dölen, E van Dinthe, L Vimeux, M Fallet, V Feuillet, CG Figdor Cross-talk between iNKT cells and CD8 T cells in the spleen requires the IL-4/CCL17 axis for the generation of short-lived effector cells Proc. Natl. Acad. Sci. U.S.A., 2019-12-03;0(0):. 2019-12-03 [PMID: 31796596] (Confocal Microscopy, Mouse) Confocal Microscopy Mouse
S Yi, J Zhai, R Niu, G Zhu, M Wang, J Liu, H Huang, Y Wang, X Jing, L Kang, W Song, Y Shi, H Tang Eosinophil recruitment is dynamically regulated by interplay among lung dendritic cell subsets after allergen challenge Nat Commun, 2018-09-24;9(1):3879. 2018-09-24 [PMID: 30250029] (Neutralization, Mouse) Neutralization Mouse
Araujo-Pires A, Vieira A, Francisconi C, Biguetti C, Glowacki A, Yoshizawa S, Campanelli A, Trombone A, Sfeir C, Little S, Garlet G IL-4/CCL22/CCR4 axis controls regulatory T-cell migration that suppresses inflammatory bone loss in murine experimental periodontitis. J Bone Miner Res, 2015-03-01;30(3):412-22. 2015-03-01 [PMID: 25264308] (Neutralization, Mouse) Neutralization Mouse
Loebbermann , Jens, Durant , Lydia, Thornton , Hannah, Johansson , Cecilia, Openshaw , Peter J Defective immunoregulation in RSV vaccine-augmented viral lung disease restored by selective chemoattraction of regulatory T cells. Proc Natl Acad Sci U S A, 2013-02-04;110(8):2987-92. 2013-02-04 [PMID: 23382205] (Neutralization, Mouse) Neutralization Mouse
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 2010-02-08;120(3):907-23. 2010-02-08 [PMID: 20197626] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Jacobsen EA, Ochkur SI, Pero RS, Taranova AG, Protheroe CA, Colbert DC, Lee NA, Lee JJ Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells. J. Exp. Med., 2008-03-03;205(3):699-710. 2008-03-03 [PMID: 18316417] (Neutralization, Mouse) Neutralization Mouse
Show All 12 Publications.

Reviews for CCL17/TARC Antibody (AF529) (0)

There are no reviews for CCL17/TARC Antibody (AF529). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CCL17/TARC Antibody (AF529) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional CCL17/TARC Products

Blogs on CCL17/TARC.

Check out the latest blog posts on CCL17/TARC.
Signal Transducer and Activator of Transcription STAT6: More than a Player in Allergic Inflammation
By Jamshed Arslan, Pharm. D., PhD. What is STAT6?The cellular pathway comprising tyrosine kinase Janus Kinase (JAK) and the transcription factor STAT connect extracellular signals from various cytokines, hormones an...  Read full blog post.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog&nbsp;#AF496,filled histogram) or Normal Goat IgG Isotype Control Antibody(Catalog&nbsp;# AB-108-C, open histogram), followed by Phycoerythrin-conjugatedAnti-Goat IgG Secondary Antibody (Catalog&nbsp;#F0107).
Goat IgG Isotype Control [Unconjugated]
AB-108-C
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CCL17/TARC Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol CCL17
Uniprot