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BubR1 Antibody (OTI6E5) [DyLight 550]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI6E5
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 550

Order Details

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BubR1 Antibody (OTI6E5) [DyLight 550] Summary

Immunogen
Full-length protein expressed in 293T cell transfected with human BubR1 expression vector.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BUB1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for BubR1 Antibody (OTI6E5) [DyLight 550]

  • Bub1A
  • BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta
  • budding uninhibited by benzimidazoles 1 homolog beta (yeast)
  • EC 2.7.11.1
  • hBUBR1
  • MAD3/BUB1-related protein kinase
  • MAD3LBUB1beta
  • Mitotic checkpoint kinase MAD3L
  • Protein SSK1
  • SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Background

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (BUB1B), also known as BubR1, is a kinase involved in spindle checkpoint function. BUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. BubR-1 localizes to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation.

BubR1 antibodies are useful tools for cancer research and cell division studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol BUB1B