![Western Blot: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BubR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BubR1.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Western-Blot-NBP2-70314-img0001.jpg "Western Blot: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BubR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BubR1.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OTI6E5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full-length protein expressed in 293T cell transfected with human BUB1B expression vector |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BUB1B |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 119.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended. |
![Immunohistochemistry Smad3 [p Ser423, p Ser425] Antibody](https://images.novusbio.com/images/Smad3-[p-Ser423--p-Ser425]-Antibody-Immunohistochemistry-NBP1-77836-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence Smad3 [p Ser423, p Ser425] Antibody](https://images.novusbio.com/images/Smad3-[p-Ser423--p-Ser425]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77836-img0007.jpg)
![Western Blot Smad3 [p Ser423, p Ser425] Antibody](https://images.novusbio.com/images/nbp1-77836_rabbit-polyclonal-smad3-p-ser423-p-ser425-antibody-24520231052432.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for BubR1 Antibody (NBP2-70314)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | BUB1B |
![Immunocytochemistry/Immunofluorescence: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BubR1.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70314-img0003.jpg "Immunocytochemistry/Immunofluorescence: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BubR1.")
![Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded pancreas tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70314-img0009.jpg "Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded pancreas tissue using anti-BubR1 mouse monoclonal antibody.")
![Flow Cytometry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - HEK293T cells transfected with either pCMV6-ENTRY BubR1.(Red) or empty vector control plasmid (Blue) were immunostaining with anti-BubR1 mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70314-img0002.jpg "Flow Cytometry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - HEK293T cells transfected with either pCMV6-ENTRY BubR1.(Red) or empty vector control plasmid (Blue) were immunostaining with anti-BubR1 mouse monoclonal, and then analyzed by flow cytometry.")
![Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70314-img0004.jpg "Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BubR1 mouse monoclonal antibody.")
![Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70314-img0005.jpg "Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BubR1 mouse monoclonal antibody.")
![Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70314-img0006.jpg "Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-BubR1 mouse monoclonal antibody.")
![Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded colon tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70314-img0007.jpg "Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded colon tissue using anti-BubR1 mouse monoclonal antibody.")
![Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded endometrium tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70314-img0008.jpg "Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded endometrium tissue using anti-BubR1 mouse monoclonal antibody.")
![Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded prostate tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70314-img0010.jpg "Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded prostate tissue using anti-BubR1 mouse monoclonal antibody.")
![Immunocytochemistry Mxi1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Mxi1_AF4185_Immunocytochemistry__Immunofluorescence_20286.jpg)
![Immunohistochemistry Cyclin B1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyclin_B1_AF6000_Immunohistochemistry_11792.jpg)
![Western Blot Cyclin B1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyclin_B1_AF6000_Western_Blot_9526.jpg)
![Simple Western Cyclin B1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyclin_B1_AF6000_Simple_Western_20094.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")