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BubR1 Antibody (OTI6E5) [DyLight 405]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI6E5
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 405

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70314AF350
Alexa Fluor 405
NBP2-70314AF405
Alexa Fluor 488
NBP2-70314AF488
Alexa Fluor 532
NBP2-70314AF532
Alexa Fluor 594
NBP2-70314AF594
Alexa Fluor 647
NBP2-70314AF647
Alexa Fluor 700
NBP2-70314AF700
Alexa Fluor 750
NBP2-70314AF750
Allophycocyanin
NBP2-70314APC
Biotin
NBP2-70314B
DyLight 350
NBP2-70314UV
DyLight 488
NBP2-70314G
DyLight 550
NBP2-70314R
DyLight 594
NBP2-70314DL594
DyLight 650
NBP2-70314C
DyLight 680
NBP2-70314FR
DyLight 755
NBP2-70314IR
FITC
NBP2-70314F
HRP
NBP2-70314H
Janelia Fluor 549
NBP2-70314JF549
Janelia Fluor 646
NBP2-70314JF646
PE
NBP2-70314PE
PerCP
NBP2-70314PCP
Unconjugated
NBP1-47680
mFluor Violet 450 SE
NBP2-70314MFV450
mFluor Violet 500 SE
NBP2-70314MFV500
mFluor Violet 610 SE
NBP2-70314MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-47680
Azide and BSA Free
NBP2-70314
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BubR1 Antibody (OTI6E5) [DyLight 405] Summary

Immunogen
Full-length protein expressed in 293T cell transfected with human BubR1 expression vector.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BUB1B
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for BubR1 Antibody (OTI6E5) [DyLight 405]

  • Bub1A
  • BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta
  • budding uninhibited by benzimidazoles 1 homolog beta (yeast)
  • EC 2.7.11.1
  • hBUBR1
  • MAD3/BUB1-related protein kinase
  • MAD3LBUB1beta
  • Mitotic checkpoint kinase MAD3L
  • Protein SSK1
  • SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Background

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (BUB1B), also known as BubR1, is a kinase involved in spindle checkpoint function. BUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. BubR-1 localizes to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation.

BubR1 antibodies are useful tools for cancer research and cell division studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BubR1 Antibody (NBP2-70314V) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Research Areas for BubR1 Antibody (NBP2-70314V)

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Blogs on BubR1

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Contact Information

Product PDFs

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  PDF Datasheet
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Calculators

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Bioinformatics

Gene Symbol BUB1B