Bub1 Antibody Summary
Immunogen |
Recombinant protein encompassing a sequence within the N-terminus region of human Bub1. The exact sequence is proprietary. |
Localization |
Nucleus, Kinetochore |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
BUB1 |
Purity |
Antigen Affinity-purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/ Immunofluorescence 1:100-1:1000
- Western Blot 1:1000-1:10000
|
Theoretical MW |
122 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Packaging, Storage & Formulations
Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
Buffer |
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol |
Preservative |
0.01% Thimerosal |
Purity |
Antigen Affinity-purified |
Alternate Names for Bub1 Antibody
Background
Chromosome segregation and mitotic checkpoints are regulated by various proteins among them are BUB1 and BUBR1/BUB1B (Budding Uninhibited by Benzimidazoles 1). During mitosis both BUB1 and BUBR1 proteins are localized to kinetochores and inhibit the anaphase stage until all chromosomes are correctly attached to the spindle. These proteins were found due to their homology to Bub1 protein from yeast. Human BUBR1 contains CD1 and CD2 domains that are found in human, mouse and yeast BUB1.3-5 The human and mouse BUBR1 proteins are 29% identical in these regions. The kinetochore localization and Bub3 binding is found in the CD1 region while the kinase domain is found in the CD2 domain. While both human and mouse BUB1 contain a nuclear localization site between CD1 and CD2 regions, the yeast protein does not contain this site. BUB1 is expressed in many tissues with the highest expression in testis and thymus, lower expression in colon and small intestine and very low expression in spleen and ovary. BUB1 expression is in correlation with the expression of MAD2, another human spindle assembly checkpoint protein.3-5 BUBR1 is mainly expressed in testis, thymus and bone marrow while in differentiated tissues such as brain, heart and muscle the expression is low.5
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
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