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ATP6V1D Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: ATP6V1D Antibody [NBP2-49254] - Analysis of human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli.
Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-49254] - Staining of human liver.
Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-49254] - Staining of human kidney shows strong luminal membranous positivity in proximal tubules.
Independent Antibodies: Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-49254] - Staining of human kidney, liver, lymph node and testis using Anti-ATP6V1D antibody NBP2-49254 (A) shows similar protein ...read more
Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-49254] - Staining of human lymph node.
Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-49254] - Staining of human kidney.
Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-49254] - Staining of human testis.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

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ATP6V1D Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: VNKAQVKIRAKKDNVAGVTLPVFEHYHEGTDSYELTGLARGGEQLAKLKRNYAKAVELLVELASLQTSFVTLDEAIKIT
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP6V1D
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ATP6V1D Recombinant Protein Antigen (NBP2-49254PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ATP6V1D Antibody

  • ATP6M
  • ATPase, H+ transporting lysosomal, member M
  • ATPase, H+ transporting, lysosomal (vacuolar proton pump)
  • ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D
  • H(+)-transporting two-sector ATPase, subunit M
  • vacuolar ATP synthase subunit D
  • vacuolar H-ATPase subunit D
  • vacuolar proton pump D subunit
  • vacuolar proton pump delta polypeptide
  • Vacuolar proton pump subunit D
  • vacuolar proton-ATPase subunit D
  • VATDV-ATPase D subunit
  • V-ATPase 28 kDa accessory protein
  • V-ATPase subunit D
  • VMA8
  • V-type proton ATPase subunit D

Background

ATP6V1D encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the V1 domain D subunit protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ATP6V1D