![Western Blot: Topo III beta Antibody (4F11) [H00008940-M01] - TOP3B monoclonal antibody (M01), clone 4F11 Analysis of TOP3B expression in K-562.](http://images.novusbio.com/fullsize/Topo-III-beta-Antibody-4F11-Western-Blot-H00008940-M01-img0007.jpg "Western Blot: Topo III beta Antibody (4F11) [H00008940-M01] - TOP3B monoclonal antibody (M01), clone 4F11 Analysis of TOP3B expression in K-562.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, KD |
| Clone | 4F11 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | TOP3B (AAH02432, 1 a.a. ~ 862 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MKTVLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGTFAGQPVRFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYIVLWLDCDKEGENICFEVLDAVLPVMNKAHGGEKTVFRARFSSITDTDICNAMACLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNTDKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHPYWANTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATVSHDCKYLQSTISFRIGPELFTCSGKTVLSPGFTEVMPWQSVPLEESLPTCQRGDAFPVGEVKMLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHPPFRDMKKGMGCNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAHRVRVSADTCSVCEAALLDVDFNKAKSPLPGDETQHMGCVFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRGRARRPPGKPNPRRPKDKMSALAAYFV |
| Specificity | TOP3B - topoisomerase (DNA) III beta |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TOP3B |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
![Simple Western EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Simple_Western_21454.jpg)
![Western Blot EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Western_Blot_21511.jpg)
![Immunocytochemistry EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Immunocytochemistry__Immunofluorescence_17288.jpg)
![Myoglobin Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Myoglobin Antibody [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
Research Areas for Topo III beta Antibody (H00008940-M01)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: Topo III beta Antibody (4F11) [H00008940-M01] - Analysis of TOP3B over-expressed 293 cell line, cotransfected with TOP3B Validated Chimera RNAi ( Cat # H00008940-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TOP3B monoclonal antibody (M01), clone 4F11 (Cat # H00008940-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Topo-III-beta-Antibody-4F11-Western-Blot-H00008940-M01-img0005.jpg "Western Blot: Topo III beta Antibody (4F11) [H00008940-M01] - Analysis of TOP3B over-expressed 293 cell line, cotransfected with TOP3B Validated Chimera RNAi ( Cat # H00008940-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TOP3B monoclonal antibody (M01), clone 4F11 (Cat # H00008940-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Western Blot: Topo III beta Antibody (4F11) [H00008940-M01] - Analysis of TOP3B expression in transfected 293T cell line by TOP3B monoclonal antibody (M01), clone 4F11.Lane 1: TOP3B transfected lysate(96.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Topo-III-beta-Antibody-4F11-Western-Blot-H00008940-M01-img0008.jpg "Western Blot: Topo III beta Antibody (4F11) [H00008940-M01] - Analysis of TOP3B expression in transfected 293T cell line by TOP3B monoclonal antibody (M01), clone 4F11.Lane 1: TOP3B transfected lysate(96.7 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: Topo III beta Antibody (4F11) [H00008940-M01] - Detection limit for recombinant GST tagged TOP3B is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Topo-III-beta-Antibody-4F11-Sandwich-ELISA-H00008940-M01-img0006.jpg "Sandwich ELISA: Topo III beta Antibody (4F11) [H00008940-M01] - Detection limit for recombinant GST tagged TOP3B is approximately 1ng/ml as a capture antibody.")
![N/A CXCL1/GRO alpha/KC/CINC-1 [Biotin]](https://images.novusbio.com/images2/DATA_CXCL1_DY453_ELISA_2039.jpg)
![Western Blot Ikaros/IKZF1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Ikaros_AF4984_Western_Blot_5594.jpg)
![Chromatin Immunoprecipitation (ChIP) Ikaros/IKZF1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Ikaros_AF4984_Chromatin_Immunoprecipitation_11241.jpg)
![Simple Western Ikaros/IKZF1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Ikaros_AF4984_Simple_Western_16946.jpg)
![Western Blot SPARC Antibody [Unconjugated]](https://images.novusbio.com/images2/SPARC_AF942_Western_Blot_20730.jpg)
![Immunohistochemistry SPARC Antibody [Unconjugated]](https://images.novusbio.com/images2/SPARC_AF942_Immunohistochemistry_10419.jpg)
![Immunocytochemistry SPARC Antibody [Unconjugated]](https://images.novusbio.com/images2/SPARC_AF942_Immunocytochemistry__Immunofluorescence_23321.jpg)
![Immunohistochemistry Klotho Antibody [Unconjugated]](https://images.novusbio.com/images2/Klotho_AF1819_Immunohistochemistry_6720.jpg)
![Western Blot Klotho Antibody [Unconjugated]](https://images.novusbio.com/images2/Klotho_AF1819_Western_Blot_22658.jpg)
![ELISA Klotho Antibody [Unconjugated]](https://images.novusbio.com/images2/Klotho_AF1819_ELISA_22841.jpg)
