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ATP6V0D2 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600] - Analysis in human kidney and pancreas tissues. Corresponding ATP6V0D2 RNA-seq data are presented for the same tissues.
Western Blot: ATP6V0D2 Antibody [NBP2-31600] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Kidney tissue
Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600] - Staining of human tonsil shows no positivity in germinal center and non-germinal center cells as expected.
Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600] - Staining of human kidney shows high expression.
Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600] - Human kidney tissue. Heat mediated antigen retrieval was performed by heating in citrate buffer (pH 6) at 95C for 20 minutes. Image from verified customer ...read more
Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600] - Staining of human small intestine shows no positivity in glandular cells as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

ATP6V0D2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: EDRETLYPTFGKLYPEGLRLLAQAEDFDQMKNVADHYGVYKPLFEAVGGSG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP6V0D2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
ATP6V0D2 Recombinant Protein Antigen (NBP2-31600PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP2-31600 in the following applications:

Publications
Read Publication using
NBP2-31600 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ATP6V0D2 Antibody

  • ATP6D2
  • ATP6V0
  • ATPase, H+ transporting, lysosomal 38kD, V0 subunit d isoform 2
  • ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d isoform 2
  • ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d2
  • FLJ38708
  • Vacuolar proton pump subunit d 2
  • V-ATPase subunit d 2
  • VMA6
  • V-type proton ATPase subunit d 2

Background

ATP6V0D2 - ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d isoform 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATP6V0D2 Antibody (NBP2-31600)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
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Filter By Species
Human
(1)
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Review for ATP6V0D2 Antibody (NBP2-31600) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-31600:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin ATP6V0D2 NBP2-31600
Enlarge
5
reviewed by:
Santhosh Sivajothi
IHC-P Human 05/22/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman kidney tissue
SpeciesHuman
LotR73682

Comments

CommentsHeat mediated antigen retrieval was performed by heating in citrate buffer (pH6) at 95C for 20 minutes

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ATP6V0D2 Antibody (NBP2-31600) (0)

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mFluor Violet Conjugated Antibodies

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Santhosh Sivajothi
05/22/2018
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol ATP6V0D2
Uniprot