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TOMM34 Antibody (OTI2A9)

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Western Blot: TOMM34 Antibody (2A9) [NBP2-00892] Analysis of extracts (35ug) from 9 different cell lines by using anti-TOMM34 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: TOMM34 Antibody (2A9) [NBP2-00892] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TOMM34.
Immunohistochemistry-Paraffin: TOMM34 Antibody (2A9) [NBP2-00892] - Staining of paraffin-embedded Human prostate tissue using anti-TOMM34 mouse monoclonal antibody.
Flow Cytometry: TOMM34 Antibody (2A9) [NBP2-00892] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TOMM34 antibody, and then ...read more
Western Blot: TOMM34 Antibody (2A9) [NBP2-00892] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOMM34 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: TOMM34 Antibody (2A9) [NBP2-00892] - Immunofluorescent staining of HeLa cells using anti-TOMM34 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TOMM34 Antibody (2A9) [NBP2-00892] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TOMM34 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TOMM34 Antibody (2A9) [NBP2-00892] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TOMM34 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TOMM34 Antibody (2A9) [NBP2-00892] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TOMM34 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TOMM34 Antibody (2A9) [NBP2-00892] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TOMM34 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TOMM34 Antibody (2A9) [NBP2-00892] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TOMM34 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TOMM34 Antibody (2A9) [NBP2-00892] - Staining of paraffin-embedded Human colon tissue using anti-TOMM34 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI2A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.96 mg/ml

Order Details

TOMM34 Antibody (OTI2A9) Summary

Immunogen
Full length human recombinant protein of human TOMM34(NP_006800) produced in HEK293 cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TOMM34
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
34.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-00892 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.96 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TOMM34 Antibody (OTI2A9)

  • hTom34
  • HTOM34P
  • mitochondrial import receptor subunit TOM34
  • outer mitochondrial membrane translocase (34kD)
  • TOM34
  • Translocase of outer membrane 34 kDa subunit
  • translocase of outer mitochondrial membrane 34
  • URCC3

Background

The protein encoded by this gene is involved in the import of precursor proteins into mitochondria. The encoded protein has a chaperone-like activity, binding the mature portion of unfolded proteins and aiding their import into mitochondria. This protein, which is found in the cytoplasm and sometimes associated with the outer mitochondrial membrane, has a weak ATPase activity and contains 6 TPR repeats. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TOMM34 Antibody (NBP2-00892)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Filter By Species
Human
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TOMM34 Antibody (NBP2-00892) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TOMM34
Entrez